Antiproliferative effect of berberine on canine mammary gland cancer cell culture

Abstract

Abstract. Canine mammary gland tumors are the most frequent cause of cancer in female dogs. Numerous studies using cancer cell lines and clinical trials have indicated that various natural products and antioxidants reduce or possibly prevent the development of cancer. Berberine (BBR), the most important alkaloid in the Berberidaceae, which exerts a wide range of pharmacological and biochemical effects, has drawn much attention due to its particularly high antitumor activity in vitro and in animal studies. The aim of the present study was to investigate the antiproliferative effect of BBR against a canine mammary gland carcinoma cell line (CF41.Mg) in vitro. CF41.Mg cells were cultured in RPMI-1640 medium containing 10% heat inactived fetal bovine serum (FBS) and 100 mg/ml peniciline-streptomycin. Subsequently the cells were treated with different concentrations of BBR chloride (10, 25, 50, 100 and 200 µM) at a density of 12,000 cells/well in 96-well plates. Following treatment, the MTT assay was used to detect cell viability after 24-, 48-and 72-h incubations at 37˚C with 5% CO 2 . The results indicated that BBR inhibited proliferation of canine mammary gland carcinoma cells, as treatment with 100 µM BBR for 24 h resulted in a significant decrease in cell viability (P<0.005). As the present study demonstrated that BBR (10-200 µM) induced cancer cell death, it is proposed that BBR may serve as a candidate agent against canine mammary tumor cells via its antiproliferative activity.

Berberine ameliorates cellular senescence and extends the lifespan of mice via regulating p16 and cyclin protein expression

“…Above that, BBR (>5 μg/ml ≈ 14.865 μ m ) inhibited cell proliferation. This result was in agreement with previous studies showing that BBR at concentrations of 10 μ m or higher inhibited cell proliferation in a dose‐dependent manner in cancer cells (Sefidabi, Mortazavi, & Hosseini, ). However, there are no reports on the effects of low concentrations berberine on cell proliferation.…”

“…One possible concern about BBR would be if it increases the risk of cancer by promoting cell proliferation. Though we showed BBR promotes cell proliferation in WI38, it is reported previously BBR treatment promotes cell‐cycle arrest and death in human cancer cell lines, coupled to an increased expression of apoptotic factors (Sefidabi et al, ). BBR was reported to leading to apoptosis in around 20 types of cancers through various mechanisms.…”

Effects of resveratrol, curcumin, berberine and other nutraceuticals on aging, cancer development, cancer stem cells and microRNAs

“…These studied used BBR concentration ranging from 10-200 micromolar. Treatment with 10-200 micromolar BBR inhibited proliferation [ 236 ].…”

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“…Recent research has shown that berberine has diverse promising biological actions against metabolic disorders, microbial infections, as an anti-oxidant, hepatoprotective, anti-arrhythmic, anti-malarial, hypolipidemic, hypoglycemic, antiproliferative, and antineoplastic activities, etc. [3][4][5][6][7] QUICK RESPONSE CODE DOI: 10.13040/IJPSR.0975-8232. 10(8).…”