Berberine attenuates arthritis in adjuvant-induced arthritic rats associated with regulating polarization of macrophages through AMPK/NF-кB pathway

Zhou, Jing; Yu, Yun; Yang, Xue; Wang, Ying; Song, Yining; Wang, Qing; Chen, Zhuo; Zong, Shiye; Fan, Min; Meng, Xue; Xie, Changhao; Zhou, Feiya; Líu, Hao; Fang, Wei

doi:10.1016/j.ejphar.2019.02.036

Cited by 53 publications

(27 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…29 In sum, berberine was found to exert the anti-arthritic effect on AIA rats by modulating the polarization of macrophages through the AMPK/NF-кB pathway. 29 Therefore, it can be concluded that drugs targeting the regulation of the macrophage polarization may become a valuable prospective therapeutic approach for RA. factor RORγt in the spleen.…”

Section: Modulatory Effect Of Berberine On Inflammatory M1 Macrophagesmentioning

confidence: 93%

“…95,96 It has been also shown that peritoneal macrophages in AIA rats are mainly of the M1 polarization status, and berberine treatment can restore the balance of the peritoneal M1/M2 by reducing the levels of M1 cytokines (TNF-α, IL-1β and IL-6), increasing the levels of M2 cytokines (IL-10 and TGF-β1), increasing the expression of Arg1 (M2 marker) and decreasing the expression of iNOS (M1 marker). 29 Mechanisms underlying the modulatory effect of berberine on macrophages are based on the theory that the inflammatory response promoted by tissue injury is closely related to metabolic regulation. AMPK is activated when the ratio of ATP/ AMP is low in cells.…”

Section: Modulatory Effect Of Berberine On Inflammatory M1 Macrophagesmentioning

confidence: 99%

See 1 more Smart Citation

Immunomodulatory effects of berberine on the inflamed joint reveal new therapeutic targets for rheumatoid arthritis management

Shen

Jiao

et al. 2020

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

Section: Modulatory Effect Of Berberine On Inflammatory M1 Macrophagesmentioning

confidence: 93%

Section: Modulatory Effect Of Berberine On Inflammatory M1 Macrophagesmentioning

confidence: 99%

Immunomodulatory effects of berberine on the inflamed joint reveal new therapeutic targets for rheumatoid arthritis management

Shen

Jiao

et al. 2020

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…Thus, we infer miR-155 affects the levels of pro-inflammatory cytokines IL-1β and TNF-α by targeting SGK3 or LXRα. The production of these cytokines, such as IL-1β and TNF-α, are necessary for the M1 polarization of microglia [53]. Thus, miR-155 possibly affects M1 polarization of microglia through indirectly regulating the production of IL-1β and TNF-α.…”

Section: Discussionmentioning

confidence: 99%

Suppression of miR-155 attenuates neuropathic pain by inducing an M1 to M2 switch in microglia

Zhang¹,

Chen²,

Nai³

et al. 2020

View full text Add to dashboard Cite

Introduction: The polarization state of microglia affects the progress of neuropathic pain. MiR-155 modulates polarization of microglia, while its role in neuropathic pain has not been well studied. Material and methods: We separately used lipopolysaccharide (LPS) and interleukin 4 (IL-4) for constructing an M1/M2 polarization model in BV-2 cells. The levels of CD86, iNOS, CD206, Arg and miR-155 were measured by western blot or qRT-PCR, as needed. Subsequently, BV-2 cells were transfected with miR-155 mimics or inhibitor to explore the effects of miR-155 on polarization states. We also constructed a neuropathic pain model by applying spinal nerve ligation (SNL) in Wistar rats with miR-155 agomir or antagomir injection. The withdrawal threshold was measured by Von Frey fibre needle. The levels of interleukin 1β (IL-1β), tumour necrosis factor α (TNF-α) and the proportion of M1-polarized microglia in primary microglia from rats were measured by ELISA and flow cytometry. Results: LPS induced M1 polarization in BV-2 cells with increasing of CD86, iNOS and miR-155, while IL-4 induced M2 polarization in BV-2 cells with increasing of CD206, Arg and decreasing of miR-155. MiR-155 mimics upregulated CD86 and downregulated CD206, whereas miR-155 inhibitor downregulated CD86 and upregulated CD206. MiR-155 antagomir increased the withdrawal threshold, decreased the production of IL-1β, TNF-α and the proportion of M1-polarized microglia in primary microglia. Conclusions: Results demonstrate that suppression of miR-155 attenuates neuropathic pain by inducing an M1 to M2 switch in microglia. Our findings provide a new perspective to understand the function of miR-155 in microglia.

show abstract

“…Previous studies have demonstrated that BBr was able to reverse chronic inflammatory pain induced by Complete Freund's adjuvant, which alleviated comorbid depression (68,69). its anti-nociceptive and anti-depressive effects may be associated with the downregulated spinal levels of the inflammatory cytokines and mrna transcription of ccl2 (70).…”

Section: Genes Involved In the Tlr4/nf-κb And Mapk/ap-1 Pathwaymentioning

confidence: 95%

Anti‑inflammatory mechanism of berberine on lipopolysaccharide‑induced IEC‑18 models based on comparative transcriptomics

Zhang

Zhao

et al. 2020

Mol Med Rep

View full text Add to dashboard Cite

intestinal surface epithelial cells (iecs) have long been considered as an effective barrier for maintaining water and electrolyte balance, and are involved in the mechanism of nutrient absorption. When intestinal inflammation occurs, it is often accompanied by iec malfunction. Berberine (BBr) is an isoquinoline alkaloid found in numerous types of medicinal plants, which has been clinically used in china to treat symptoms of gastrointestinal pathogenic bacterial infection, especially bacteria-induced diarrhea and inflammation. In the present study, iec-18 rat intestinal epithelial cells were treated with lipopolysaccharide (lPS) to establish an in vitro model of epithelial cell inflammation, and the cells were subsequently treated with BBR in order to elucidate the anti-inflammatory mechanism. Transcriptome data were then searched to find the differentially expressed genes (deGs) compared between two of the treatment groups (namely, the lPS and lPS+BBr groups), and deGs were analyzed using Gene ontology, Kyoto encyclopedia of Genes and Genomes, Weighted Gene correlation network analysis and interactive Pathways explorer to identify the functions and pathways enriched with deGs. Finally, reverse transcription-quantitative Pcr was used to verify the transcriptome data. These experiments revealed that, comparing between the lPS and lPS+BBr groups, the functions and pathways enriched in deGs were 'dna replication', 'cell cycle', 'apoptosis', 'leukocyte migration' and the 'nF-κB and aP-1 pathways'. The results revealed that BBr is able to restrict dna replication, inhibit the cell cycle and promote apoptosis. it can also inhibit the classic inflammatory pathways, such as those mediated by NF-κB and aP-1, and the expression of various chemokines to prevent the migration of leukocytes. according to transcriptomic data, BBR can exert its anti-inflammatory effects by regulating a variety of cellular physiological activities, including cell cycle, apoptosis, inflammatory pathways and leukocyte migration.

show abstract

Berberine attenuates arthritis in adjuvant-induced arthritic rats associated with regulating polarization of macrophages through AMPK/NF-кB pathway

Cited by 53 publications

References 41 publications

Immunomodulatory effects of berberine on the inflamed joint reveal new therapeutic targets for rheumatoid arthritis management

Immunomodulatory effects of berberine on the inflamed joint reveal new therapeutic targets for rheumatoid arthritis management

Suppression of miR-155 attenuates neuropathic pain by inducing an M1 to M2 switch in microglia

Anti‑inflammatory mechanism of berberine on lipopolysaccharide‑induced IEC‑18 models based on comparative transcriptomics

Contact Info

Product

Resources

About