Anti‑inflammatory mechanism of berberine on lipopolysaccharide‑induced IEC‑18 models based on comparative transcriptomics

Abstract

intestinal surface epithelial cells (iecs) have long been considered as an effective barrier for maintaining water and electrolyte balance, and are involved in the mechanism of nutrient absorption. When intestinal inflammation occurs, it is often accompanied by iec malfunction. Berberine (BBr) is an isoquinoline alkaloid found in numerous types of medicinal plants, which has been clinically used in china to treat symptoms of gastrointestinal pathogenic bacterial infection, especially bacteria-induced diarrhea and inflammation. In the present study, iec-18 rat intestinal epithelial cells were treated with lipopolysaccharide (lPS) to establish an in vitro model of epithelial cell inflammation, and the cells were subsequently treated with BBR in order to elucidate the anti-inflammatory mechanism. Transcriptome data were then searched to find the differentially expressed genes (deGs) compared between two of the treatment groups (namely, the lPS and lPS+BBr groups), and deGs were analyzed using Gene ontology, Kyoto encyclopedia of Genes and Genomes, Weighted Gene correlation network analysis and interactive Pathways explorer to identify the functions and pathways enriched with deGs. Finally, reverse transcription-quantitative Pcr was used to verify the transcriptome data. These experiments revealed that, comparing between the lPS and lPS+BBr groups, the functions and pathways enriched in deGs were 'dna replication', 'cell cycle', 'apoptosis', 'leukocyte migration' and the 'nF-κB and aP-1 pathways'. The results revealed that BBr is able to restrict dna replication, inhibit the cell cycle and promote apoptosis. it can also inhibit the classic inflammatory pathways, such as those mediated by NF-κB and aP-1, and the expression of various chemokines to prevent the migration of leukocytes. according to transcriptomic data, BBR can exert its anti-inflammatory effects by regulating a variety of cellular physiological activities, including cell cycle, apoptosis, inflammatory pathways and leukocyte migration.

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