Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots

Ríos-Ramírez, Suzel del Carmen; Valle, José Raymundo Enríquez-del; Rodríguez-Ortiz, Gerardo; Ruíz-Luna, Judith

doi:10.7764/rcia.v44i3.1810

Cited by 8 publications

(9 citation statements)

References 15 publications

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“…La interacción de los reguladores de crecimiento no fue significativa (p = 0.286) con los dos genotipos de A. maximiliana. La combinación de reguladores de crecimiento para el subgénero Agave, con dosis baja de auxina (entre 0.025 y 0.5 mg L -1 de auxina) y alta de citocinina (entre 3 y 10 mg L -1 de citocinina) se ha reportado como exitosa para la estimulación en la producción de brotes apartir de yemas axilares en A. angustifolia (Ríos-Ramírez et al, 2017), A. guiengola (Chávez-Ortiz et al, 2021 y para A. fourcroydes (Robert et al, 1992;Zhang et al, 2013); en el caso de A. guiengola se encontró una producción máxima de brotes de 43 (Chávez-Ortiz et al, 2021).…”

Section: Resultados Y Discusiónunclassified

“…Hay varias investigaciones acerca del cultivo de tejidos de diferentes especies de Agave (Robert et al, 2006): A. americana (Chen et al, 2014;Naziri et al, 2018;Cruz-García et al, 2019), A. angustifolia (Monja-Mio et al, 2015Arzate-Fernández et al, 2016;Reyes-Díaz et al, 2017;Ríos-Ramírez et al, 2017;Sánchez et al, 2020;Monja-Mio et al, 2021), A. cantala Roxburgh y A. fourcroydes Lem. (Binh et al, 1990;Monja-Mio & Robert, 2013), A. inaequidens (Aureoles-Rodríguez al., 2008), A. marmorata (Aguilar-Jimenez & Rodriguez, 2018Arzate-Fernández et al, 2020), A. salmiana (Puente-Garza et al, 2015Arzate-Fernández et al, 2020) A. sisalana Perrine (Binh et al, 1990;Hazra et al, 2002;Carneiro et al, 2014;Nikam et al, 2019), A. tequilana (Santamaría et al, 1995;Valenzuela-Sánchez et al, 2006;Portillo et al, 2007;Santacruz-Ruvalcaba & Portillo, 2009;Portillo et al, 2012;Delgado-Aceves et al, 2021), A. victoria-reginae Moore (Rodríguez-Garay et al, 1996Martínez-Palacios et al, 2003;Ramírez-Malagón et al, 2008) sin existir protocolos de propagación in vitro para A. maximiliana reportados a la fecha.…”

Section: Introductionunclassified

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Micropropagación de Agave maximiliana Baker por proliferación de yemas axilares

Santacruz-Ruvalcaba¹,

Castañeda-Nava²,

Villanueva-Gónzalez³

et al. 2022

polibotanica

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Section: Resultados Y Discusiónunclassified

Section: Introductionunclassified

Micropropagación de Agave maximiliana Baker por proliferación de yemas axilares

Santacruz-Ruvalcaba¹,

Castañeda-Nava²,

Villanueva-Gónzalez³

et al. 2022

polibotanica

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“…The plants irrigated with SNS-50%, or with SNS-50% + benzylaminopurine developed 1.6 and 1.9 rhizome offshoots, significantly higher (Duncan, 0.05) than the 1.1 shoots emitted by plants irrigated with only water. The substrate does not provide the plant with sufficient essential nutrients for optimum growth, but it can (Miguel et al, 2014) and Agave angustifolia (Ríos-Ramírez et al, 2017) the data show that the cytokinins stimulated the development of multiple shoots.…”

Section: Resultsmentioning

confidence: 99%

Plant growth and rhizome shoots of Agave angustifolia in different substrates, with fertigation and benzylaminopurine

et al. 2020

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The species Agave angustifolia can be propagated asexually with offshoots from rhizomes, bulbils from inflorescence, and through micropropagation to supply the growing demand for quality plant material for new plantations. In this investigation, Agave angustifolia plants of different initial sizes were evaluated for growth and emission of rhizome shoots, after 14 months under different conditions of substrate, fertigation and application of cytokinins. Bulbils were collected from inflorescences of plants at two sites and classified into two size categories: small (≤ 7 cm) and large (> 7 cm). The bulbils were cultured for 14 months in pots with substrates that were mixtures of sand (A), soil (S), and bovine manure (Bm) (%): 1) A + S (0.3: 0.7); 2) A + S + Bm (0.3: 0.525: 0.175); 3) A + S + Bm (0.3: 0.35: 0.35); 4) A + S + Bm (0.3: 0.175: 0.525). The plants in each substrate were separated into groups for application of different types of irrigation: 1) water; 2) 50% Steiner’s nutritive solution, SNS-50%; 3) SNS-50% + 25 mg L-1 of benzylaminopurine. The results show that the emission of rhizome offshoots was affected by the substrate, type of irrigation and provenance of the plants. The plants in substrates 3 and 4 formed 2.2 rhizome offshoots, which was more than plants in the other substrates. The plants irrigated with water, SNS-50%, and SNS-50% + benzylaminopurine formed 1.1, 1.6 and 1.9 rhizome offshoots, respectively.

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“…The in vitro micropropagation in Agave has a wide range of advantages, as recently reviewed by [ 31 ]; however, for ecological purposes, the multiplication of identical plants decreases their genetic variability when reintroduced to the forest. On the other hand, not all agave species respond efficiently to plant growth regulators to enhance shoot multiplication, and it also depends on the type of explant used [ 32 , 33 , 34 , 35 ]. Delgado-Aceves et al [ 36 ] proposed that the protocols for the multiplication of endangered agave species require modifications of other species to increase the adaptation and propagation effectiveness.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Seed-Derived Clonal Lines of the Endangered Agave marmorata Roezl and Their Compatibility with Endophytes

Martínez-Rodríguez

Beltran-Garcia

Salas

et al. 2022

Biology

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A. marmorata is the raw material used for tepextate mescal production but is classified as an endangered species. In the present study, we obtain and multiply clonal lines of Agave marmorata Roezl by selecting seedlings derived from seeds. Ten seedlings from two lots of 400 germinated seeds were selected for axillary bud proliferation induced by BAP 5 mg/L in vitamin-free Murashige and Skoog’s medium. Differences in shoot numbers, heights and senescent tissue formation were observed. Notably, the AM32 line formed 84 shoots and presented low senescent tissue after 60 d of culture. We also selected the AM31 and AM33 clonal lines. Four-month shoots were extracted with 80% methanol in water to determine the total content of saponins, flavonoids, and phenolic acids and compare the three clonal lines. Some bioactive molecules were identified using HPLC techniques and MALDI-TOF mass spectrometry none showed significant differences in content. Additionally, plants derived from the clonal lines were inoculated with four endophytic bacteria. Among these, Achromobacter xylosoxidans supported plant growth of AM32. A notable effect of plant death was observed after inoculation with Enterobacter cloacae, an endophyte of A. tequilana. Additionally, Pseudomonas aeruginosa, an endophyte from A. marmorata, reduced biomass. Our results demonstrate the incompatibility of A. marmorata to E. cloacae and specialization between the host plant and its endophytes. The compatibility of the plant-endophyte could be exploited to boost the establishment and stability of mutualisms to benefit plant development, stress tolerance and pathogen resistance. The differences in multiplication capacity, stable tissue formation, and endophyte biotization responses may indicate genetic variability. Clonal selection and micropropagation from seed-derived plants could contribute to conserving the endangered A. marmorata plant for reforestation in their natural habitats, thus, assuring mass propagation for sustainable industrial production of mescal, bioactive compounds, and prebiotics.

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Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots

Cited by 8 publications

References 15 publications

Micropropagación de Agave maximiliana Baker por proliferación de yemas axilares

Micropropagación de Agave maximiliana Baker por proliferación de yemas axilares

Plant growth and rhizome shoots of Agave angustifolia in different substrates, with fertigation and benzylaminopurine

Micropropagation of Seed-Derived Clonal Lines of the Endangered Agave marmorata Roezl and Their Compatibility with Endophytes

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