Benzyladenine-induced changes in the translatable mRNA population in excised cucumber cotyledons

Teramoto, Haruhiko; Momotani, Eiki; Tsuji, Hideo

doi:10.1034/j.1399-3054.1993.870420.x

Cited by 6 publications

(11 citation statements)

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“…For the control, water was added instead of BA. The fresh weight of cucumber cotyledons begins to increase at about 4 h after BA application, and reaches a level 40% higher than the controls at 24 h (Teramoto et al 1993). In our previous report, the analysis of in-vitro translation products showed that levels of some translatable mRNAs changed from 6 to 12 h after BA application, during which cotyledon expansion proceeded, but some changed within 6 h. Particularly, one translatable mRNA decreased to nearly undetectable levels at 2 h, and two others disappeared at 6 h. In order to investigate the primary action of cytokinins, we tried to isolate cDNA clones for mRNAs whose levels were reduced after a short period of BA treatment.…”

Section: Isolation Of Cdna Clones For Cytokinin-repressed Mrnas By DImentioning

confidence: 91%

“…Total RNA was prepared according to Teramoto et al (1993). Cotyledons were homogenized with a Physcotton-type homogenizer (Nichion Irika, Funabashi, Japan) or a Potter-Elvehjem-type glass homogenizer in GT buffer (4 M guanidine thiocyanate; 20 mM Tris-HCl, pH 8.0; 2% sarcosyl; 2% 2-mercaptoethanol) with an equal volume of a mixture of phenol: chloroform : isoamyl alcohol (25:24:1, by vol.).…”

Section: Methodsmentioning

confidence: 99%

“…In a previous paper, we analyzed in-vitro translation products of mRNA from excised cucumber cotyledons using two-dimensional PAGE and showed that BA caused changes in levels of several mRNAs, three of them being markedly repressed (Teramoto et al 1993). In the present study, we isolated a eDNA clone (CR9) for a cytokinin-repressed gene by differential screening.…”

Section: Introductionmentioning

confidence: 96%

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Isolation of a cDNA clone for a cytokinin-repressed gene in excised cucumber cotyledons

Teramoto

Momotani

Takeba

et al. 1994

Planta

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Rapid changes in gene expression were studied during incubation of cucumber (Cucumis sativus L.) cotyledons with cytokinins in darkness. Complementary-DNA clones for mRNAs whose levels decreased within 4 h of treatment with N6-benzyladenine (BA) were isolated by differential hybridization. One of them (CR9) was sequenced. It is 588 bp long, and would encode a protein consisting of 137 amino-acid residues and having a molecular mass of 15 kDa. The sequence shows a high homology with a light-induced gene from rice. Northern blot analysis of the CR9 transcript showed the level of the mRNA (0.7 kb) to decrease tenfold within 4 h of BA treatment, i.e. well before BA-induced cotyledon expansion was observed. The repression became greater with increasing concentration of BA (10(-8)-10(-5) M). The expression of the CR9 gene was repressed specifically by cytokinins (BA, isopentenyladenine and t-zeatin), but not by adenine or 2,4-dichlorophenoxyacetic acid (auxin). The results are discussed in relation to the primary action of cytokinin.

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Section: Isolation Of Cdna Clones For Cytokinin-repressed Mrnas By DImentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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Isolation of a cDNA clone for a cytokinin-repressed gene in excised cucumber cotyledons

Teramoto

Momotani

Takeba

et al. 1994

Planta

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“…47 It has been established that cytokinin-induced cotyledon expansion was dependent on nucleic acid synthesis. 48 Thomas et al…”

Section: 40mentioning

confidence: 99%

Interaction of cytokinins with UV-B (280 -315nm) on the expansion growth of cucumber cotyledons

Kataria¹,

Kn²

2018

HIJ

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The negative effect on plant responses are expected due to increased amounts of ultraviolet-B (UV-B) radiation reaching on the Earth's surface by stratospheric ozone depletion. Cytokinins (CKs) are essential phytohormones in plant growth and development. Perusal of relevant literature reveals that CKs mitigate the oxidative damage caused by abiotic stresses like salt, drought, high temperature and heavy metal due to their antioxidant effects. However, very few reports are available on the interaction of CKs with UV-B stress on the expansion growth of the cucumber cotyledons. CKs induced the expansion growth of the excised cucumber cotyledons in the darkness. CKs induced expansion growth of cucumber cotyledons in darkness was inhibited by supplemental UV-B. UV-B radiation enhanced the level of oxyradicals like superoxide and hydroxide radicals in the excised cucumber cotyledons, which was evident by EPR spectroscopy. CKs like Zeatin, TDZ, BAP and FAP reduced the level of oxyradicals produced in the dark grown cucumber cotyledons, while promoting the expansion growth of the cotyledons. Production of oxyradical in UV-B exposed cotyledons showed that these oxyradicals might partially account for inhibition of expansion growth. Since considerable amount of oxyradicals were quenched by CKs at higher concentrations (10 and 20 µg/ml) it partially restores the inhibition of expansion growth caused by UV-B stress. It indicates that overproduction of oxyradicals by UV-B could not be entirely responsible for the inhibition of CKs induced expansion growth of cotyledons; which might be caused by some other physiological changes caused by UV-B irradiation in addition to the production of oxyradicals. UV-B is also known to inactivate protein inhibitor of peroxidase in cucumber cotyledons. This may reduce the antioxidants defence and enhance the damaging effects of oxyradicals for the growth of cotyledons.

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“…Experimental evidence indicates that there is a close association between the regulation of gene expression and cytokinin action (Chen and Leisner 1985;Flores and Tobin 1988;Cotton et al 1990;Crowell et al 1990;Lu et al 1990Lu et al , 1992Dorninov et al 1992;Sugiharto et al 1992;Chert at al. 1993;Teramoto et al 1993;Binns 1994; Abbreviations: BA = N6-benzyladenine; HPR = hydroxypyruvate reductase Correspondence to: C.M. Chert; FAX: 1 (414) 595 2056; E-mail: chen@cs.uwp.edu Crowell 1994;Ye and Varner 1994).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional regulation of hydroxypyruvate reductase gene expression by cytokinin in etiolated pumpkin cotyledons

Andersen

Jin

Chen

et al. 1996

Planta

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To understand the mechanisms by which the expression of a specific gene is modulated by cytokinin, the regulation of hydroxypyruvate reductase (HPR) transcript levels by N6-benzyladenine (BA) in etiolated pumpkin (Cucurbita pepo L. cv. Halloween) cotyledons was investigated. A pumpkin HPR cDNA was generated by reverse transcriptase-polymerase chain reaction and its nucleotide sequence was determined. An antisense HPR RNA was prepared for RNase protection analysis of HPR-mRNA expression patterns in the cotyledons of dark-grown pumpkin seedlings. Treatment of the cotyledons with BA was shown to modulate HPR mRNA levels in a dose- and time-dependent manner. Similarly, nuclear run-on studies showed that the rate of transcription was also enhanced by BA treatment of the cotyledons. These results suggest that the enhancement of HPR mRNA by cytokinin is, at least in part, at the level of transcription.

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Benzyladenine-induced changes in the translatable mRNA population in excised cucumber cotyledons

Cited by 6 publications

References 0 publications

Isolation of a cDNA clone for a cytokinin-repressed gene in excised cucumber cotyledons

Isolation of a cDNA clone for a cytokinin-repressed gene in excised cucumber cotyledons

Interaction of cytokinins with UV-B (280 -315nm) on the expansion growth of cucumber cotyledons

Transcriptional regulation of hydroxypyruvate reductase gene expression by cytokinin in etiolated pumpkin cotyledons

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