Benzoylurea resistance in western flower thrips &lt;i&gt;Frankliniella occidentalis&lt;/i&gt; (Thysanoptera: Thripidae): the presence of a point mutation in &lt;i&gt;chitin synthase 1&lt;/i&gt;

Suzuki, Youhei; Shiotsuki, Takahiro; Jouraku, Akiya; Miura, Ken; Minakuchi, Chieka

doi:10.1584/jpestics.d17-023

Cited by 20 publications

(22 citation statements)

References 23 publications

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“…Recent studies reported the same nonsynonymous mutation in the CHS gene of the CSI-resistant arthropods: the mite ( Tetranychus urticae ), the moth ( Plutella xylostella) , and the thrip ( Frankliniella occidentalis ) [10–12]. This mutation is a substitution changing isoleucine to methionine or phenylalanine, and is not found in the catalytic active site of CHS but in a C-terminal transmembrane region, which is highly conserved, and could play an essential function for the enzyme.…”

Section: Discussionmentioning

confidence: 99%

“…Since 1970, when the first benzoylurea was introduced on the market, many suggested that CHS could be the target of CSIs. Recently a nonsynonymous mutation in the CHS sequence has been reported in a few CSIs-resistance arthropods [10–12]. However, neither in vivo nor in vitro studies have shown inhibition of the catalytic activity of CHS by CSIs [13,14].…”

Section: Introductionmentioning

confidence: 99%

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Effects of chitin synthesis inhibitor treatment on Lepeophtheirus salmonis (Copepoda, Caligidae) larvae

et al. 2019

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The salmon louse (Lepeophtheirus salmonis) is an ectoparasite infecting Atlantic salmon (Salmo salar), which causes substantial problems to the salmon aquaculture and threatens wild salmon. Chitin synthesis inhibitors (CSIs) are used to control L. salmonis in aquaculture. CSIs act by interfering with chitin formation and molting. In the present study, we investigated the action of four CSIs: diflubenzuron (DFB), hexaflumuron (HX), lufenuron (LF), and teflubenzuron (TFB) on larval molt. As the mode of action of CSIs remains unknown, we selected key enzymes in chitin metabolism and investigated if CSI treatment influenced the transcriptional level of these genes. All four CSIs interfered with the nauplius II molt to copepodids in a dose-dependent manner. The EC50 values were 93.2 nM for diflubenzuron, 1.2 nM for hexaflumuron, 22.4 nM for lufenuron, and 11.7 nM for teflubenzuron. Of the investigated genes, only the transcriptional level of L. salmonis chitin synthase 1 decreased significantly in hexaflumuron and diflubenzuron-treated larvae. All the tested CSIs affected the molt of nauplius II L. salmonis larvae but at different concentrations. The larvae were most sensitive to hexaflumuron and less sensitive to diflubenzuron. None of the CSIs applied had a strong impact on the transcriptional level of chitin synthesis or chitinases genes in L. salmonis. Further research is necessary to get more knowledge of the nature of the inhibition of CSI and may require methods such as studies of protein structure and enzymological studies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of chitin synthesis inhibitor treatment on Lepeophtheirus salmonis (Copepoda, Caligidae) larvae

et al. 2019

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“…Resistance to mite growth inhibitors and chitin synthase inhibitors has been attributed to the I1017F mutation in chitin synthase (Demaeght et al., 2014; Douris et al., 2016; Grigoraki et al., 2017; Riga et al., 2017; Van Leeuwen et al., 2012). As the I1017F mutation is located in a highly conserved region of the CHS1 gene (Douris et al., 2016; Grigoraki et al., 2017; Suzuki, Shiotsuki, Jouraku, Miura, & Minakuchi, 2017; Yamamoto, Yoneda, Hatano, & Asada, 1995) , this substitution could impair the enzymatic function. In the current study, the impact of I1017F on spider mite fitness was quantified by analyzing single‐generation life‐history traits, fertility life table parameters of individual near‐isogenic lines as well as by an interline competition experiment.…”

Section: Discussionmentioning

confidence: 99%

Fitness costs of key point mutations that underlie acaricide target‐site resistance in the two‐spotted spider miteTetranychus urticae

Bajda

Riga

Wybouw

et al. 2018

Evolutionary Applications

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The frequency of insecticide/acaricide target‐site resistance is increasing in arthropod pest populations and is typically underpinned by single point mutations that affect the binding strength between the insecticide/acaricide and its target‐site. Theory predicts that although resistance mutations clearly have advantageous effects under the selection pressure of the insecticide/acaricide, they might convey negative pleiotropic effects on other aspects of fitness. If such fitness costs are in place, target‐site resistance is thus likely to disappear in the absence of insecticide/acaricide treatment, a process that would counteract the spread of resistance in agricultural crops. Hence, there is a great need to reliably quantify the various potential pleiotropic effects of target‐site resistance point mutations on arthropod fitness. Here, we used near‐isogenic lines of the spider mite pest Tetranychus urticae that carry well‐characterized acaricide target‐site resistance mutations to quantify potential fitness costs. Specifically, we analyzed P262T in the mitochondrial cytochrome b, the combined G314D and G326E substitutions in the glutamate‐gated chloride channels, L1024V in the voltage‐gated sodium channel, and I1017F in chitin synthase 1. Five fertility life table parameters and nine single‐generation life‐history traits were quantified and compared across a total of 15 mite lines. In addition, we monitored the temporal resistance level dynamics of populations with different starting frequency levels of the chitin synthase resistant allele to further support our findings. Three target‐site resistance mutations, I1017F and the co‐occurring G314D and G326E mutations, were shown to significantly and consistently alter certain fitness parameters in T. urticae. The other two mutations (P262T and L1024V) did not result in any consistent change in a fitness parameter analyzed in our study. Our findings are discussed in the context of the global spread of T. urticae pesticide resistance and integrated pest management.

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“…Indeed, field populations of Tetranychus urticae Koch (Tetranychidae: Trombidiformes) [ 55 ], and Plutella xylostella (L.) (Plutellidae: Lepidoptera) [ 56 ] have been found that are resistant to etoxazole, which has a similar mode of action as novaluron. These and Frankliniella occidentalis (Thysanoptera: Thripidae) have been demonstrated to have achieved resistance through point mutations in chitin synthase 1 [ 57 ]. To reduce the likelihood that stored product pests would begin to exhibit resistance when novaluron is applied to surfaces frequently, application rates may need to be greater 0.209 mg/m 2 .…”

Section: Discussionmentioning

confidence: 99%

Residual Efficacy of Novaluron Applied on Concrete, Metal, and Wood for the Control of Stored Product Coleopteran Pests

Yasir

Mankin

Hasan

et al. 2020

Insects

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The residual efficacy of novaluron on concrete, metal and wood was evaluated against last-instar larvae of Oryzaephilus surinamensis (L.), Triboliumcastaneum (Herbst), and Trogoderma granarium Everts. The larvae and food provided for survival were exposed to surfaces pretreated at rates of 0.053, to 0.209 mg/m2 and bioassays were conducted from 0- to 16-weeks post-treatment. Percentage emergence of adults was recorded after 30 days (d). On all surfaces at week 0, no O. surinamensis or T.castaneum adults emerged, and ≤3.3% emergence of T. granarium was found at 0.209 mg/m2. Novaluron significantly reduced the percentage emergence of adults of the three species compared to a distilled water control for the first 12 weeks on all the tested surfaces as the residual efficacy declined at a low rate during initial weeks and then at a high rate in the final weeks of the 16-week study. Reductions to emergence were most persistent on metal surfaces, with mean percentages of adult emergence of ≤18.3 in week 12, followed by concrete (≤32.5) and wood (≤45.0) for all species at novaluron application rates of 0.209 mg/m2. For >4-weeks protection, higher application rates would be needed to avoid buildup of pest populations and reductions in profitability. Such results can be helpful for the management of O. surinamensis, T.castaneum, and T. granarium as the costs of commonly used insecticides against postharvest insect pests and the resistance of these insects to the pesticides gradually increase in mills, warehouses, and food storage facilities.

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Benzoylurea resistance in western flower thrips <i>Frankliniella occidentalis</i> (Thysanoptera: Thripidae): the presence of a point mutation in <i>chitin synthase 1</i>

Cited by 20 publications

References 23 publications

Effects of chitin synthesis inhibitor treatment on Lepeophtheirus salmonis (Copepoda, Caligidae) larvae

Effects of chitin synthesis inhibitor treatment on Lepeophtheirus salmonis (Copepoda, Caligidae) larvae

Fitness costs of key point mutations that underlie acaricide target‐site resistance in the two‐spotted spider miteTetranychus urticae

Residual Efficacy of Novaluron Applied on Concrete, Metal, and Wood for the Control of Stored Product Coleopteran Pests

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