Benzo(a)pyrene promotes A549 cell migration and invasion through up-regulating Twist

Wang, Yadong; Zhai, Wenlong; Li, Ang; Xia, Xiangqun; Zhang, Congke

doi:10.1007/s00204-014-1269-8

Cited by 46 publications

(36 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A wound healing assay was performed according to the method described in our previous study (17). Briefly, monolayer cells were wounded by scratching the surface of each well in a 6-well plate as uniformly as possible with a 200 µl pipette tip.…”

Section: Methodsmentioning

confidence: 99%

“…The wells were then rinsed with phosphate-buffered saline (PBS) three times and were incubated at 37°C for 48 h. Images of the initial wound, and the movement of cells into the scratched area, were captured using a Leica DM IL LED inverted microscope equipped with a digital imaging system (Leica Microsystems GmbH, Wetzlar, Germany) at 0 and 48 h. The wound width of 5 random views was measured. The healing width was calculated as wound width at 0 h minus wound width at 48 h, and was normalized to A549-shcon cells (17). …”

Section: Methodsmentioning

confidence: 99%

“…Following a 24 h incubation at 37°C, cells on the upper-side of the membrane were removed using clean swabs, and cells on the underside were viewed and counted under a Leica DM IL LED inverted microscope (Leica Microsystems GmbH). The number of invaded cells was counted in 5 randomly selected fields (17). …”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

STIM1 silencing inhibits the migration and invasion of A549 cells

Wang

Pan

et al. 2017

Molecular Medicine Reports

Self Cite

View full text Add to dashboard Cite

The present study aimed to explore the effects of stromal interaction molecule 1 (STIM1) knockdown on the migration, invasion and metastasis of A549 cells in vitro and in vivo. Western blotting and immunohistochemistry were used to detect protein expression levels. Wound healing and Transwell invasion assays were used to assess the migratory and invasive abilities of A549 cells transfected with STIM1-specific short hairpin (sh)RNA (shSTIM1). In addition, a tail vein metastatic assay was performed. The results demonstrated that the frequency of STIM1 high-expression was significantly increased in metastatic lung cancer tissues (72.2%) compared with in non-metastatic lung cancer tissues (33.0%). STIM1 knockdown inhibited A549 cell migration and invasion in vitro and tumor metastasis in vivo. The protein expression levels of Snail1, Vimentin, matrix metalloproteinase (MMP) 2 and MMP9 were markedly decreased in A549-shSTIM1 compared with in A549 cells transfected with control shRNA (shcon). In addition, the protein expression levels of E-cadherin were markedly increased in A549-shSTIM1 cells compared with in A549-shcon cells. These results suggested that STIM1 knockdown may inhibit the migration and invasion of A549 cells in vitro, and metastasis in vivo.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

STIM1 silencing inhibits the migration and invasion of A549 cells

Wang

Pan

et al. 2017

Molecular Medicine Reports

Self Cite

View full text Add to dashboard Cite

show abstract

“…57 Recently, some studies provided mechanistic clues showing that BaP may contribute to lung cancer cell invasion and metastasis by upregulating proinflammatory chemokines (IL8, CCL-2, CCL-3) and the one of the master regulator of the epithelial-to-mesenchymal transition, Twist. 58,59 Moreover, BaP was able to induce cell migration in triple-negative breast cancer MDA-MB-231 cells through a lipoxygenase-and Src-dependent pathway, notably by increasing the secretion of metalloproteinase MMP-2 and MMP-9. 60 BaP treatment was also able to increase the metastatic potential of hepatocellular carcinoma cell lines in a mouse model, likely through an activation of both angiogenesis and NF-kB pathway.…”

Section: Hallmark 6: Activating Invasion and Metastasismentioning

confidence: 99%

Filling the gap between chemical carcinogenesis and the hallmarks of cancer: A temporal perspective

Demetriou

Esposti

Fedinick

et al. 2018

Eur J Clin Investigation

View full text Add to dashboard Cite

show abstract

“…BaP causes DNA adduct formation, which is the initiating event in carcinogenesis (7). BaP has also been demonstrated to promote A549 cell migration and invasion by upregulating Twist (8).…”

Section: Introductionmentioning

confidence: 99%

Laricitrin suppresses increased benzo(a)pyrene-induced lung tumor-associated monocyte-derived dendritic cell cancer progression

et al. 2016

View full text Add to dashboard Cite

Abstract. Benzo(a)pyrene (BaP) stimulates lung cancer cells, promoting monocyte-derived dendritic cells to secrete soluble factors, including heparin binding-epidermal growth factor and C-X-C motif chemokine 5. The secretions from monocyte-derived dendritic cells stimulate the progression of lung cancer cells, including the migration and invasion of cells. To the best of our knowledge, these secretions remain unknown, and require additional study. The present study identified that treatment with BaP-H1395-tumor-associated dendritic cell-conditioned medium had the most marked effect on cell migration and invasion. This result may be associated with the female gender, stage 2 adenocarcinoma or mutation of the proto-oncogene B-Raf (BRAF), according to the cell line background. Laricitrin, a dietary flavonoid derivative present in grapes and red wine, suppresses certain factors and decreases the progression of lung cancer cells that are promoted by BaP in the lung cancer tumor microenvironment. The results of the present study suggest that prolonged exposure to BaP exacerbates lung cancer, particularly in female lung cancer patients with the BRAF mutation, but that laricitrin may ameliorate this effect.

show abstract

Benzo(a)pyrene promotes A549 cell migration and invasion through up-regulating Twist

Cited by 46 publications

References 18 publications

STIM1 silencing inhibits the migration and invasion of A549 cells

STIM1 silencing inhibits the migration and invasion of A549 cells

Filling the gap between chemical carcinogenesis and the hallmarks of cancer: A temporal perspective

Laricitrin suppresses increased benzo(a)pyrene-induced lung tumor-associated monocyte-derived dendritic cell cancer progression

Contact Info

Product

Resources

About