Benzanthrone induced immunotoxicity via oxidative stress and inflammatory mediators in Balb/c mice

Tewari, Prachi; Roy, Ruchi; Mishra, Sakshi; Mandal, Papiya; Chaudhari, Bhushan P.; Chaturvedi, Rajnish Kumar; Dwivedi, Premendra D.; Das, Mukul

doi:10.1016/j.imbio.2014.10.011

Cited by 18 publications

(14 citation statements)

References 63 publications

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“…MPO activity is commonly used to estimate in the initiation and progression of acute and chronic inflammatory diseases. 37,38 Our present findings show that NAP exposure resulted in an enhancement of the MPO activity in the livers and lungs and DATS inhibited the expression of MPO. The content determination data are consistent with the results of the immunohistochemistry.…”

Section: Discussionsupporting

confidence: 58%

Protective effect of diallyl trisulfide against naphthalene-induced oxidative stress and inflammatory damage in mice

Zhang

Wang

et al. 2016

Int J Immunopathol Pharmacol

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The aim of this study was to investigate the possible protective effects of diallyl trisulfide (DATS) against naphthalene-induced oxidative and inflammatory damage in the livers and lungs of mice. Elevated serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels showed significant hepatic damage after the challenge with 100 mg/kg naphthalene. Hepatic malondialdehyde (MDA) contents and the activity of myeloperoxidase (MPO) increased significantly, accompanying a decrease in the hepatic activity of total superoxide dismutase (SOD) and glutathione (GSH) levels after the naphthalene damage. In addition, the serum levels of nitric oxide (NO), tumor necrosis factor α (TNF-α), and interleukin 8 (IL-8) increased significantly in the groups damaged with naphthalene. The main parameters related to oxidative stress and inflammatory responses in the lungs, including the NO, MPO, and GSH contents, were determined, and the histopathological and immunohistochemical changes in the lung and liver tissues were also observed. In the DATS-treated groups, all of the oxidative and inflammatory damage in the serum, liver, and lung tissues were significantly prevented.

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Section: Discussionsupporting

confidence: 58%

Protective effect of diallyl trisulfide against naphthalene-induced oxidative stress and inflammatory damage in mice

Zhang

Wang

et al. 2016

Int J Immunopathol Pharmacol

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“…For example, subchronic treatment with malathion and carbosulfan in rat resulted in moderate but significant changes in spleen IL‐4 and IFN‐γ production . Similar effects were observed in mRNA levels of cytokines in the spleen of mice exposed to benzanthrone . It could be possible that these chemicals, including 8:2 FTOH, may cause more significant changes in immune responses upon stimulation, as shown in Figure .…”

Section: Discussionmentioning

confidence: 65%

“…In addition, xenobiotics usually show dose‐dependent toxic effects in organisms. For example, the spleen of mice exposed to 15 mg/kg/d benzanthrone for 7 days exhibited noteworthy hyperplasia and megakaryocytes infiltration, whereas no noticeable histological change was observed in the spleen of mice exposed to 3.5 mg/kg/d benzanthrone . It is possible that the exposure of 8:2 FTOH at a higher dose (>100 mg/kg/d) could cause morphological changes in the spleen and thymus.…”

Section: Discussionmentioning

confidence: 99%

8:2 Fluorotelomer alcohol causes immunotoxicity and liver injury in adult male C57BL/6 mice

Wang

Kong

et al. 2018

Environmental Toxicology

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8:2 Fluorotelomer alcohol (8:2 FTOH) is widely used in houseware and industrial goods and is ubiquitous in the surrounding environment. 8:2 FTOH has been linked to hepatoxicity, nephrotoxicity, and reproductive toxicity, as well as endocrine-disrupting effects. However, as of yet, the research regarding immunotoxicity of 8:2 FTOH remains largely limited. In the present study, adult male C57BL/6 mice were administered with 10, 30, and 100 mg/kg/d 8:2 FTOH by gavage for 28 days to investigate its immunotoxicity in vivo. The results showed that exposure to 8:2 FTOH caused increases in liver weight and histological changes in the liver, including vacuolation, cell swelling, immune cell infiltration, karyopyknosis and nuclear swelling. No histological change in either the spleen or the thymus was observed after administration of 8:2 FTOH. In addition, exposure to 8:2 FTOH reduced the concentration of IL-1β in serum, and mRNA levels of IL-1β, IL-6, and TNF-α in both the thymus and spleen. CXCL-1 mRNA expression was downregulated in both the liver and thymus after 8:2 FTOH administration, while only IL-1β mRNA expression was upregulated in the liver. Moreover, the exposure of primary cultured splenocytes to 8:2 FTOH inhibited the ConA-stimulated proliferation of splenocytes at concentrations of 30 and 100 μM, and the LPS-stimulated proliferation of splenocytes at 100 μM. Furthermore, 8:2 FTOH inhibited the level of secreted IFN-γ in ConA-stimulated splenocytes. The results obtained in the study demonstrated that 8:2 FTOH posed potential immunotoxicity and liver injury in mice. Our findings will provide novel data for the health risk assessment of 8:2 FTOH. K E Y W O R D S 8:2 FTOH, immunotoxicity, liver injury, mice, oxidative stress 1 | INTRODUCTION Fluorotelomers, as fluorocarbon-based oligomers or telomers, have various applications in food packaging, home furnishings, fire-fighting foams, surfactants, textiles, adhesives, waxes, polishes, and leather. 1 It was reported that the market size of fluorotelomers was 26.5 kt in 2015, and its annual growth rate is estimated to be 12.7%. 2 Among fluorotelomers, fluorotelomer alcohol (FTOH) products dominate global consumption; these products reportedly made up approximately 32% of the overall volume of fluorotemomers in 2013. 3 8:2 FTOH is the most abundant FTOH homologue and is ubiquitous in the environment. 4 Chen et al. 5 detected FTOHs in several wastewater treatment plants in China, and the study also found that 8:2 FTOH was the primary FTOH in the influent, secondary effluent, and sludge, with the concentrations ranging from 2.10 to 11.0 ng/L, 3.05 to 12.4 ng/L, and 0.36 to 1.91 ng/g dry weight, respectively. Bach et al. 6 assessed 14 perfluorinated compounds (PFCs) in water samples in France and found that the concentrations of 8:2 FTOH were 117 ng/L and 213 ng/L in tap and surface water, respectively. Additionally, 8:2 FTOH was commonly detected in food contact materials (FCMs). For example, 8:2 FTOH was detected in most Chinese and American FCM samples at concentra...

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“…Because myeloperoxidase (MPO) activity can reflect the degree of neutrophil infiltration and thus may be used as an index for evaluating the severity of CHS, 36 we examined the MPO activity of ear homogenates obtained from CHS mice 72 hr post-injection. Compared with the control group, MPO activity was notably decreased in the MSC CXCR5 group and differed slightly in the MSC EGFP group (Figure 5C).…”

Section: Msc Cxcr5 Infusion Dramatically Ameliorates Chsmentioning

confidence: 99%

CXCR5-Overexpressing Mesenchymal Stromal Cells Exhibit Enhanced Homing and Can Decrease Contact Hypersensitivity

et al. 2017

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Mesenchymal stromal cells (MSCs) can modulate inflammation and contribute to tissue regeneration and, thus, have emerged as a promising option for cell-based therapy. However, the ability of MSCs to migrate to injured tissues still needs to be improved. In this study, we investigated whether genetically engineered MSCs could exhibit increased migratory properties and improved therapeutic efficacy. Using a mouse model of contact hypersensitivity (CHS), chemokine gene expression screening revealed that CXCL13 changed most significantly in injured tissue. Unfortunately, MSCs hardly express the corresponding receptor, CXCR5. Thus, CXCR5-overexpressing MSCs (MSC) were generated that retained their abilities of proliferation, differentiation, and immunomodulation. Furthermore, MSC showed significantly increased migrating ability toward CXCL13. Importantly, systemic infusion of MSC dramatically suppressed CHS in mice, as evidenced by decreased levels of inflammatory cell infiltration and pro-inflammatory cytokine production. Numerous MSC migrated into inflamed ears, localized with T cells, inhibited T cell proliferation, promoted T cell apoptosis, and suppressed the production of T cell-derived pro-inflammatory factors. Collectively, these findings demonstrate that CXCR5 overexpression increases the ability of MSCs to respond to migratory stimuli and highly intensifies their immunomodulatory effects in vivo. This strategy for enhancing targeted stem/progenitor cell homing may improve the efficacy of MSC-based therapies.

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Benzanthrone induced immunotoxicity via oxidative stress and inflammatory mediators in Balb/c mice

Cited by 18 publications

References 63 publications

Protective effect of diallyl trisulfide against naphthalene-induced oxidative stress and inflammatory damage in mice

Protective effect of diallyl trisulfide against naphthalene-induced oxidative stress and inflammatory damage in mice

8:2 Fluorotelomer alcohol causes immunotoxicity and liver injury in adult male C57BL/6 mice

CXCR5-Overexpressing Mesenchymal Stromal Cells Exhibit Enhanced Homing and Can Decrease Contact Hypersensitivity

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