Benzamidine-sensitive proteinase in activated cleavage of recombinant 74 kDa histidine decarboxylase into its 53 kDa form in mastocytoma cells

Ichikawa, Atsushi; Funakoshi, Eishi; Tanaka, Satoshi; Nemoto, Koji; Fukui, Tetsuya

doi:10.1007/s000110050264

Cited by 4 publications

(4 citation statements)

References 6 publications

(7 reference statements)

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“…Benzamidine-sensitive proteinase is activated at an alkaline pH (pH 8-9) (Ichikawa et al, 1998;Demartini et al, 2007). In the present study, the pH values of pruritogenic 1% and 10% sodium laurate were 9.8 and 10.1, respectively, whereas those of nonpruritogenic 0.1% sodium laurate and 10% N-lauroylsarcosine sodium salt were 7.6 and 7.7, respectively.…”

Section: Surfactant Itch and Keratinocyte Histaminementioning

confidence: 45%

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Topical Surfactant-Induced Pruritus: Involvement of Histamine Released from Epidermal Keratinocytes

Inami

Andoh

Sasaki

et al. 2012

J Pharmacol Exp Ther

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Surfactants, an important component of cleansers, often cause itch in humans. Topical application of sodium laurate and Nlauroylsarcosine sodium salt to the skin of mice immediately (for 1-1.5 hours) increased scratching, and the former increased scratching again between 2 and 3 hours after application. Thus, we examined the mechanisms of sodium laurate-induced delayed scratching. Sodium laurate (0.1%-10%) increased delayed scratching and skin surface pH in a concentrationdependent manner. N-lauroylsarcosine sodium salt had no effect on these parameters, and sodium hydroxide solution did not increase delayed scratching. Sodium laurate-induced delayed scratching was markedly inhibited by the H 1 histamine receptor antagonist terfenadine, but it was not affected by mast cell deficiency. Sodium laurate application had no effect on the number of total and degranulated mast cells, and did not induce plasma extravasation or the infiltration of inflammatory cells in the skin. Sodium laurate application increased the histamine content of the epidermis, but not that of the dermis, in normal and mast cell-deficient mice. Sodium laurate application increased the ratio of 53-kDa L-histidine decarboxylase (HDC, a key enzyme for histamine production) to 74-kDa HDC in the mouse epidermis and in a human keratinocyte culture. Sodium laurate increased histamine in the human keratinocyte culture, without affecting cell viability. The present results suggest that sodium laurate induced delayed scratching at an alkaline pH through the increased production of histamine in keratinocytes, which may be due to enhanced processing of 74-kDa to 53-kDa HDC.

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Section: Surfactant Itch and Keratinocyte Histaminementioning

confidence: 45%

“…Although details of the mechanisms of HDC processing remain unclear, benzamidine-sensitive proteinase has been reported to be involved in HDC processing (Ichikawa et al, 1998). Benzamidine-sensitive proteinase is activated at an alkaline pH (pH 8-9) (Ichikawa et al, 1998;Demartini et al, 2007).…”

Section: Surfactant Itch and Keratinocyte Histaminementioning

confidence: 99%

Topical Surfactant-Induced Pruritus: Involvement of Histamine Released from Epidermal Keratinocytes

Inami

Andoh

Sasaki

et al. 2012

J Pharmacol Exp Ther

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“…Although details of the mechanisms of HDC processing remain unclear, benzamidine-sensitive proteinase has been reported to be involved in HDC processing (49). Benzamidine-sensitive proteinase is activated at an alkaline pH (pH 8-9) (49,50). The pH values of the SDS and N-lauroylsarcosine sodium salt solutions were 6.5 and 7.7, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…These findings suggest the possibility that increased pH plays a role in the mechanism of the post-translation processing of HDC. Although details of the mechanisms of HDC processing remain unclear, benzamidine-sensitive proteinase has been reported to be involved in HDC processing (49). Benzamidine-sensitive proteinase is activated at an alkaline pH (pH 8-9) (49,50).…”

Section: Discussionmentioning

confidence: 99%

Surfactant-induced Chronic Pruritus: Role of L-Histidine Decarboxylase Expression and Histamine Production in Epidermis

Inami¹,

Sasaki²,

Andoh³

et al. 2014

Acta Derm Venerol

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Shampoo and cleansers containing anionic surfactants including sodium dodecyl sulphate (SDS) often cause pruritus in humans. Daily application of 1-10% SDS for 4 days induced hind-paw scratching (an itch-related behaviour) in a concentration-dependent manner, and 10% SDS also caused dermatitis, skin dryness, barrier disruption, and an increase in skin surface pH in mice. SDS-induced scratching was inhibited by the opioid receptor antagonist naloxone and the H histamine receptor antagonist terfenadine. Mast-cell deficiency did not inhibit SDS-induced scratching, although it almost completely depleted histamine in the dermis. Treatment with SDS increased the histamine content of the epidermis, but not that of the dermis. SDS treatment increased the gene expression and post-translation processing of L-histidine decarboxylase in the epidermis. The present results suggest that repeated application of SDS induces itch through increased production of epidermal histamine, which results from an increase in the gene expression and post-translation processing of L-histidine decarboxylase.

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界面活性剤によって誘発されるかゆみとケラチノサイト-ヒスタミン系の関与

et al. 2012

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The primary function of surfactants is to remove dirt, exfoliated corneum cells, and microorganisms from the skin. However, the use of toiletries such as soaps and shampoos containing surfactants may cause adverse eŠects such as cutaneous irritation, dryness, and itching. Recently, skin pathologies, including dry skin, rough skin, and sensitive skin, have increased because of changes in living conditions and lifestyle. Although many people with skin pathologies complain of itching during and/or after skin washing using detergents, the mechanisms of detergent-induced itch are yet to be elucidated. Therefore, in this study, we investigated the mechanisms underlying surfactant-induced itching. We found that topical application of an anionic surfactant sodium laurate at an alkaline pH, but not N-lauroylsarcosine sodium salt at neutral pH, to mouse skin induced scratching, an itch-related response. Additionally, we found that the sodium laurate-induced scratching was inhibited by H 1 histamine receptor antagonist, but not mast cell deˆciency. Sodium laurate application increased histamine content and the level of the active form (53 kDa) of L-histidine decarboxylase (HDC) in the mouse epidermis, but not the dermis. Furthermore, addition of sodium laurate to a human epidermal cell culture increased histamine release and HDC levels, without aŠecting cell viability. These results suggest that surfactants with alkaline properties are pruritogenic and that the pruritus is induced by the histamine released from epidermal keratinocytes. The increase in histamine release may be attributable to the activation of HDC in epidermal keratinocytes.

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Benzamidine-sensitive proteinase in activated cleavage of recombinant 74 kDa histidine decarboxylase into its 53 kDa form in mastocytoma cells

Cited by 4 publications

References 6 publications

Topical Surfactant-Induced Pruritus: Involvement of Histamine Released from Epidermal Keratinocytes

Topical Surfactant-Induced Pruritus: Involvement of Histamine Released from Epidermal Keratinocytes

Surfactant-induced Chronic Pruritus: Role of L-Histidine Decarboxylase Expression and Histamine Production in Epidermis

界面活性剤によって誘発されるかゆみとケラチノサイト-ヒスタミン系の関与

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