Benefits and Limitations of Protein Hydrolysates as Components of Serum-Free Media for Animal Cell Culture Applications

Lobo-Alfonso, J. O.; Price, Paul J.; Jayme, David W.

doi:10.1007/978-1-4020-6674-0_4

Cited by 10 publications

(13 citation statements)

References 45 publications

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“…Hydrolysates, as a source of free amino acids, are being used as supplements for basal medium, to sustain cell growth and to allow cell adaptation to serum-free culture media. Hydrolysates can enrich the nutritional profile of the media by increasing the stability of glutamine, and processes other beneficial bioactivities (Table 3 ), leading to enhanced cell viable density (Lobo-Alfonso et al 2010 ; Ng et al 2020 ). One critical drawback of using hydrolysates as media supplements is that they are undefined, leading to batch-to-batch quality issues.…”

Section: The Use Of Hydrolysate In Animal Cell Culturementioning

confidence: 99%

“…For example, specific yeast peptides from yeast hydrolysate and beta-glucan particularly (1,3)- β -glucan in the cell wall from yeast and plant-based hydrolysates are potentially immunogenic (Jiang et al 2011 ). In addition, hydrolysates derived from microbes may contain high levels of lipopolysaccharide (LPS), an endotoxin that is difficult to completely remove from the final product (Lobo-Alfonso et al 2010 ). Hydrolysates that are derived from Gram-negative bacteria like Escherichia coli would likely contain a high level of LPS contamination, which may require a more stringent post-processing procedure for LPS removal (Xu et al 2019 ).…”

Section: Contaminants and By-products Of Hydrolysatementioning

confidence: 99%

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Applications and analysis of hydrolysates in animal cell culture

Lim

et al. 2021

Bioresour. Bioprocess.

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Animal cells are used in the manufacturing of complex biotherapeutic products since the 1980s. From its initial uses in biological research to its current importance in the biopharmaceutical industry, many types of culture media were developed: from serum-based media to serum-free to protein-free chemically defined media. The cultivation of animal cells economically has become the ultimate goal in the field of biomanufacturing. Serum serves as a source of amino acids, lipids, proteins and most importantly growth factors and hormones, which are essential for many cell types. However, the use of serum is unfavorable due to its high price tag, increased lot-to-lot variations and potential risk of microbial contamination. Efforts are progressively being made to replace serum with recombinant proteins such as growth factors, cytokines and hormones, as well as supplementation with lipids, vitamins, trace elements and hydrolysates. While hydrolysates are more complex, they provide a diverse source of nutrients to animal cells, with potential beneficial effects beyond the nutritional value. In this review, we discuss the use of hydrolysates in animal cell culture and briefly cover the composition of hydrolysates, mode of action and potential contaminants with some perspectives on its potential role in animal cell culture media formulations in the future.

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Section: The Use Of Hydrolysate In Animal Cell Culturementioning

confidence: 99%

Section: Contaminants and By-products Of Hydrolysatementioning

confidence: 99%

Applications and analysis of hydrolysates in animal cell culture

Lim

et al. 2021

Bioresour. Bioprocess.

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“…Many CD media are optimized for one CHO cell type and may offer reduced growth support for others . A further problem with CD media may be the low tolerance of the cultures to small molecular toxic components such as inorganic ions that otherwise would be sequestered by the presence of proteins or peptides . However, the addition of hydrolysates to chemically defined media can be used to increase protein production .…”

Section: Introductionmentioning

confidence: 99%

“…However, the addition of hydrolysates to chemically defined media can be used to increase protein production . The CD media may contain recombinant proteins that are relatively expensive, and may increase production costs . Therefore, a goal in defined media production is to isolate or define new media supplements that are effective with many cell lines and allow high productivity of recombinant proteins, and are available at a reasonable cost.…”

Section: Introductionmentioning

confidence: 99%

“…2,27,28 A further problem with CD media may be the low tolerance of the cultures to small molecular toxic components such as inorganic ions that otherwise would be sequestered by the presence of proteins or peptides. 29 However, the addition of hydrolysates to chemically defined media can be used to increase protein production. 23,30,31 The CD media may contain recombinant proteins that are relatively expensive, and may increase production costs.…”

Section: Introductionmentioning

confidence: 99%

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The bioactivity and fractionation of peptide hydrolysates in cultures of CHO cells

Spearman

Lodewyks

Richmond

et al. 2014

Biotechnology Progress

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Peptide hydrolysate supplements in mammalian cell cultures provide enhanced growth and productivity. The objective of this study was to compare the bioactivity of ten different commercially available hydrolysates from plant, microbial, and animal sources. The peptide hydrolysates were tested as supplements to cultures of Chinese hamster ovary (CHO) cells that produce human beta interferon (β-IFN). A soy hydrolysate was shown to support high cell growth but not protein productivity compared to an animal component hydrolysate (Primatone RL). On the other hand, a yeast hydrolysate showed lower cell growth, but comparable productivity of the recombinant protein. Glycosylation analysis showed that the glycan profile of β-IFN produced in yeast hydrolysate supplemented cultures was equivalent to that from Primatone RL-supplemented cultures. Fractionation of the yeast hydrolysate and Primatone RL produced a similar protein-assayed pattern except for one extra peak at around 1 kDa in the Primatone RL profile. A fraction taken at a molecular weight range of 1.5-1.7 kDa showed the highest growth promoting activity in both samples. However, four other fractions in yeast hydrolysate and two in Primatone RL at lower molecular weights showed some growth promoting activity. In conclusion, the yeast hydrolysates provided a good alternative to the animal sourced Primatone RL for high productivity of β-IFN from CHO cells with equivalent glycosylation.

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Introduction to Animal Cell Culture

Soccol,

Dos Santos Costa

et al. 2024

Cultivated Meat

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Benefits and Limitations of Protein Hydrolysates as Components of Serum-Free Media for Animal Cell Culture Applications

Cited by 10 publications

References 45 publications

Applications and analysis of hydrolysates in animal cell culture

Applications and analysis of hydrolysates in animal cell culture

The bioactivity and fractionation of peptide hydrolysates in cultures of CHO cells

Introduction to Animal Cell Culture

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