Benefit of Prestorage Leukocyte Depletion of Single‐Donor Platelet Concentrates

Chalandon, Yves; Mermillod, Bernadette; Beris, P.; Doucet, Arlette; Chapuis, B; Roux‐Lombard, Pascale; Dayer, Jean‐Michel

doi:10.1046/j.1423-0410.1999.7610027.x

Cited by 22 publications

(23 citation statements)

References 38 publications

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“…WBC content is another important factor in plateletpheresis today. Earlier leukodepletion may offer advantages since it is associated with a lesser degree of cytokine and biologic response modifier (BRM) generation, leading to fewer FNHTR [7,10,11,26,27]. More than 85% of our collection products meet the AABB criteria for leukoreduction.…”

Section: Discussionmentioning

confidence: 99%

“…Filtration and more recently prestorage filtration have been used to diminish WBC contamination, the latter procedure is also aimed at reducing generation of cytokines such as interleukins-8, -1␤, and -6 (IL-8, IL-1␤, IL-6), and tumor necrosis factor-␣ (TNF-␣) in the transfusion product [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…There have been several reports investigating the quality of platelets prepared by plateletpheresis, followed by filtration and the possible advantages of prestorage filtration [1,7,9]. More recently a few reports and many abstracts have been published on the quality of platelets obtained with modern third generation cell separators providing leukodepleted products [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…We used CD62P fluorescein isothiocyanate (FITC)-conjugated (Immunotech-Marseille, France) mouse anti-human P-selectin as activation marker. Briefly, as described earlier [7,19], 2 l of PC (2 × 10 6 platelets) in 40 l Hepes buffer was incubated with 10 l CD62P and 10 l CD42b at room temperature in the dark for 30 min. The samples were fixed with 1 ml of 1% paraformaldehyde.…”

mentioning

confidence: 99%

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Quality evaluation of plateletpheresis using the new AMICUS (Baxter) cell separator: Evolution of CD 62 expression

et al. 1998

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Quality evaluation of plateletpheresis using the new AMICUS (Baxter) cell separator: Evolution of CD 62 expression

et al. 1998

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“…Moreover, WBC contamination during PC collection was higher than that with the other instruments. Both artificial membranes 28 and WBC 29 are known to activate platelets and to induce a storage lesion in PCs that could justify their lower in vivo efficacy.…”

Section: Leukemiamentioning

confidence: 99%

Factors influencing post-transfusional platelet increment in pediatric patients given hematopoietic stem cell transplantation

Salvaneschi

Klersy

et al. 2001

Leukemia

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Patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) always require platelet transfusions, but the increase in platelet count is often less than expected. Since factors responsible for poor response to platelet transfusions in this clinical setting are largely unknown, we performed a prospective study in 87 consecutive children transplanted in a single institution. The mean 16-h corrected count increment (CCI) of 598 platelet transfusions was 5.76 ± 8.32 × 10 9 /l. Both before and after HSCT, 13.8% of patients had antibodies against HLA and/or platelet-specific antigens. Univariate analysis identified 12 factors significantly associated with a lower post-transfusion CCI, but only four reached statistical significance in the multivariate analysis. These four factors were concomitant therapy with vancomycin, alloimmunization, use of an Autopheresis cell separator for preparation of platelet concentrates and cytomegalovirus infection. We, therefore, suggest that a better response to platelet transfusions could be obtained by choosing a suitable cell separator, by avoiding the use of vancomycin and by adopting measures that reduce alloimmunization and CMV infection. Moreover, screening patients for HLA and platelet-specific antibodies before HSCT would identify the majority of subjects who will develop alloimmune refractoriness after transplantation and would allow the search for a compatible donor in advance. Leukemia (2001) 15, 1885-1891.

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Altering interface detector positioning in combination with prestorage filtration to achieve a better quality of single donor platelet concentrates using the CS 3000 plus blood separator

Lydaki

Nikoloudi

Bolonaki

et al. 2002

J of Clinical Apheresis

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The interface detector (ID) is an optical density sensor that affects the quality of single donor platelet collection using the CS 3000 Plus blood separator. The purpose of this study was to evaluate the effect of altering ID position on platelet yields and the contamination of leukocytes (WBC) in platelet concentrates (PCs). Dual-needle apheresis procedures (n = 93) were performed using an A35 collection chamber. Plateletpheresis products were separated according to interface detector offset (IDO) positioning into four groups: A: IDO = 6 (n = 33), B: IDO = 10 (n = 28), C: IDO = 12 (n = 18), D: IDO = 18 (n = 14). For 32% of the collections, the closed system apheresis kit with integral Sepacell filter (Baxter) was used and 33% of them were leukodepleted using the LRP-6 (PALL) filter. Our results showed that: (1) Although the mean blood volume and the time of apheresis were significantly higher, the mean platelet (PLT) yields were significantly lower in PCs of group A as compared to all other groups (P < 0.0001). (2) The mean WBC content was significantly higher in PCs of group D as compared to all other groups (P < 0.0001). (3) With the LRP-6 filter, a significantly higher WBC reduction as well as PLT loss in PCs was observed as compared to Sepacell leukapheresis filter. A higher PLT loss was observed with both filters when leukoreduction was performed within the first 6 hours as compared to 24 hours after the procedure. Conclusively, an IDO setting of 10 or 12 results in better platelet yields in PCs without increasing the WBC contamination. An IDO positioning of 18 or higher must be avoided or should be always combined with PCs leukodepletion. Finally, the best timing for leukoreduction is 24 hours after the plateletpheresis.

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Benefit of Prestorage Leukocyte Depletion of Single‐Donor Platelet Concentrates

Cited by 22 publications

References 38 publications

Quality evaluation of plateletpheresis using the new AMICUS (Baxter) cell separator: Evolution of CD 62 expression

Quality evaluation of plateletpheresis using the new AMICUS (Baxter) cell separator: Evolution of CD 62 expression

Factors influencing post-transfusional platelet increment in pediatric patients given hematopoietic stem cell transplantation

Altering interface detector positioning in combination with prestorage filtration to achieve a better quality of single donor platelet concentrates using the CS 3000 plus blood separator

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