Beneficial properties of selenium incorporated guar gum nanoparticles against ischemia/reperfusion in cardiomyoblasts (H9c2)

Abstract

Nanotechnology for the treatment and diagnosis has been emerging recently as a potential area of research and development. In the present study, selenium incorporated guar gum nanoparticles have been prepared by nanoprecipitation and characterized by transmission electron microscopy and particle size analysis. The nanoparticles were screened for antioxidant potential (metal chelation, total reducing power and hydroxyl radical scavenging activity) and were evaluated against the cell line based cardiac ischemia/reperfusion model with special emphasis on oxidative stress and mitochondrial parameters. The cell based cardiac ischemia model was employed using H9c2 cell lines. Investigations revealed that there was a significant alteration (P ≤ 0.05) in the innate antioxidant status (glutathione↓, glutathione peroxidase↓, thioredoxin reductase↓, superoxide dismutase↓, catalase↓, lipid peroxidation↑, protein carbonyl↑, xanthine oxidase↑ and caspase 3 activity↑), mitochondrial functions (reactive oxygen species generation, membrane potential, and pore opening) and calcium homeostasis (calcium ATPase and intracellular calcium overload) during both ischemia and reperfusion. For comparative evaluation, selenium, guar gum and selenium incorporated guar gum nanoparticles were evaluated for their protective properties against ischemia/reperfusion. The study reveals that selenium incorporated guar gum nanoparticles were better at protecting the cells from ischemia/reperfusion compared to selenium and guar gum nanoparticles. The potent antioxidant capability shown by the sample in in vitro assays may be the biochemical basis of its better biological activity. Further, the nanodimensions of the particle may be the additional factor responsible for its better effect.

Phloretin ameliorates arsenic trioxide induced mitochondrial dysfunction in H9c2 cardiomyoblasts mediated via alterations in membrane permeability and ETC complexes

“…Reactive oxygen species play an important role in the development of cardiovascular disease, including hypertension, atherosclerosis, cardiac hypertrophy, ischemia-reperfusion injury and stroke (Paravicini and Touyz 2008;Prathapan et al, 2014;Soumya et al, 2014). In addition to increased free radical production in the failing heart, there is evidence that decreased antioxidant reserves contribute to increased oxidative stress.…”

Sodium selenite/selenium nanoparticles (SeNPs) protect cardiomyoblasts and zebrafish embryos against ethanol induced oxidative stress

“…For this, H9C2 cells were seeded in a 35mm plate at a density of 10,000 cells per well, subjected to 1% ethanol, and then treated with sodium selenite and SeNPs. Then the cells were incubated with DCFH-DA at 37°C for 20 min [9].…”

“…The working stain (100 µg mL -1 of AO and 100 µg mL -1 of EtBr in phosphate buffered saline) was added to cells and then examined under fluorescent microscope [9].…”

“…(1) Group I (Control): H9C2 were incubated in normal medium supplemented with 10% FBS and 5% CO2 during the entire experimental period [9].…”

Selenium – a fascinating antioxidant of protective properties

“…52 Another study performed on cardiomyoblast H9c2 cells revealed the occurrence of changes in the antioxidant level as well as in mitochondrial functions during ischemia and reperfusion which were prevented by selenium in the form of selenium incorporated guar gum nanoparticles. 53 Natural selenium forms have also been found to show a protective effect under in vitro conditions. Two seleniumenriched medicines of herbal origin (IMOD and Angipars) (Rose Pharmed Biotechnology Co., Iran) were studied in an experiment performed on human lymphocytes.…”