Beneficial effects of Stevioside on AGEs, blood glucose, lipid profile and renal status in streptozotocin-induced diabetic rats

Abstract

The advanced glycated end products (AGEs) are formed in the diabetic patients; it is a major cause of macrovascular and microvascular complications in diabetes. Clinically there is no treatment available for the AGEs. Stveoside (Stv), a sweetener has potent anti-diabetic and anti-oxidant activity. Hence, we investigated its use in prevention of AGEs formation using in vitro and in vivo models. Diabetes was induced by streptozotocin (STZ). These rats were kept without treatment till blood HbA1c was markedly increased. They were then divided into 5 groups and treated orally with vehicle or Metformin (MET) or Stv respectively for 28 days. Every 7th day, animals were tested for body weight and blood glucose (BG). On the last day of treatment, all the groups were evaluated for physiological and biochemical parameters, histopathology and AGEs; N-carboxymethyl-lysine (CML) estimation. Stv showed inhibition of AGEs in in vitro as well as in in vivo respectively. Positive effects were seen on the BG, lipid profile and urine parameters as well it showed reduced formation of CML. It also showed antihyperglycaemic, antihyperlipedemic and nephroprotective activities. The present study provides scientific rationale for the use of Stv as a sweetener with additional benefits in diabetes.

Potential medicinal effects and applications of stevia constituents

“…Recently, Aswar et al reported positive effects of stevioside 50 and 100 mg/kg on advanced glycated end products, blood glucose and lipid profile in streptozotocin-induced diabetic rats (Aswar et al 2019). It is interesting to note that a very recent report on the effect of steviol glycosides and steviol on streptozotocin diabetic mice verified that the potency of the effect obeyed the following sequence: steviol [ steviol glucosyl ester [ steviolbioside [ rubusoside [ stevioside [ rebaudioside A.…”

Effect of Spacing on Growth, Yield and Chemical Composition of Stevia Plants (Stevia rebaudiana Bert.)

“…Furthermore, studies on rats revealed that the consumption of stevia leaves can decrease glycaemia and gluconeogenesis [5]. In a similar study performed by Aswar et al (2019) [6], the consumption of stevioside had positive effects on blood glucose levels and lipid profiles as well as on advanced glycated end products. A study performed on humans, with a group consisting of lean and obese individuals, revealed that the consumption of stevia (compared with sucrose or aspartame) has shown positive effects, such as reduced postprandial glucose levels or postprandial Appl.…”