Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis

Abstract

In our previous study, human fibroblast growth factor 1 was successfully fused with oleosomes, energy-storing organelles of seeds, which are considered to be excellent “expression carriers” for substances with a convenient purification process. The present work aimed to explore the beneficial effects of oleosomes fused with human fibroblast growth factor 1 (OLAF) on wound healing. The data showed marked improvements in terms of the angiogenesis, vascular integrity, collagen and inflammation on the wound sites of rats with a full-thickness skin defect. Moreover, the positive role of OLAF in promoting angiogenesis and its possible pathways were clarified in vivo and in vitro. The results showed that the number, length and branches of the blood vessels of the chick embryo chorioallantoic membrane were markedly increased after OLAF treatment. Meanwhile, the in vitro results also revealed that 100 ng/mL OLAF exhibited a promoting effect on the proliferation, migration and tube formation of human umbilical vein endothelial cells. In addition, the potential of OLAF to improve wound angiogenesis was demonstrated to be associated with an up-regulated PI3K/Akt pathway by transcriptome sequencing analysis and the introduction of a PI3K/Akt pathway inhibitor (LY294002). These findings suggest that OLAF has many prospects in the development of drugs for wound healing.

Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells

“…9 C). Wound healing and angiogenesis involve a substantial number of signaling proteins, such as chemokines, cytokines, growth factors (GFs), and matrix metalloproteinases (MMPs) [ 52 , [57] , [58] , [59] ]. VEGF, TGFB1, and HGF are widely recognized for their ability to repair and regenerate the dermis and epidermis, regulate inflammation, and promote angiogenesis.…”

MXene/TPU Hybrid Fabrics Enable Smart Wound Management and Thermoresponsive Drug Delivery

“…The skin, being the largest organ of the human body, plays a crucial role in defending against external pathogens and toxins while maintaining optimal water and heat balance within the body. − Therefore, the timely repair of wounds is essential for maintaining the normal physiological levels of the human body. − The persistent inflammation triggered by bacterial infection hinders wound healing and leads to the development of chronic wounds, imposing a significant burden on global healthcare systems and economies. − Currently, the utilization of wound dressings remains the primary approach for managing wounds in clinical practice. , However, conventional wound dressings (such as gauze, , bandages, etc.) merely passively deliver medications and protect the wound.…”