Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells

Zhou, Zixuan; Xun, Jingnan; Wu, Chenghao; Ji, Chao; Ji, Shizhao; Shu, Futing; Wang, Yuxiang; Chen, Hao; Zheng, Yongjun; Xiao, Shichu

doi:10.1016/j.mtbio.2023.100686

Cited by 4 publications

(1 citation statement)

References 63 publications

(71 reference statements)

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“…Gene-activated scaffolds (GASs) transfected with genes were loaded with bone marrow-derived mesenchymal stem cells (BM-MSCs) to form GAS/BM-MSCs constructs; these constructs were shown to accelerate the wound healing process and induce in situ regeneration of full-layer skin with sweat glands [154]. The use of micronized amniotic membrane (mAM) as a microcarrier can improve the in vitro expansion efficiency of mesenchymal stem cells [155]. Studies have shown that the pre-treatment of MSCs with bioactive compounds can improve their survival rate and regenerative potential.…”

Section: Other Practicable Methods To Deliver Stem Cellsmentioning

confidence: 99%

The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration

Wu,

Sun,

et al. 2024

Biomedicines

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Mesenchymal stem cells (MSCs) have been recognized as a cell therapy with the potential to promote skin healing. MSCs, with their multipotent differentiation ability, can generate various cells related to wound healing, such as dermal fibroblasts (DFs), endothelial cells, and keratinocytes. In addition, MSCs promote neovascularization, cellular regeneration, and tissue healing through mechanisms including paracrine and autocrine signaling. Due to these characteristics, MSCs have been extensively studied in the context of burn healing and chronic wound repair. Furthermore, during the investigation of MSCs, their unique roles in skin aging and scarless healing have also been discovered. In this review, we summarize the mechanisms by which MSCs promote wound healing and discuss the recent findings from preclinical and clinical studies. We also explore strategies to enhance the therapeutic effects of MSCs. Moreover, we discuss the emerging trend of combining MSCs with tissue engineering techniques, leveraging the advantages of MSCs and tissue engineering materials, such as biodegradable scaffolds and hydrogels, to enhance the skin repair capacity of MSCs. Additionally, we highlight the potential of using paracrine and autocrine characteristics of MSCs to explore cell-free therapies as a future direction in stem cell-based treatments, further demonstrating the clinical and regenerative aesthetic applications of MSCs in skin repair and regeneration.

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Section: Other Practicable Methods To Deliver Stem Cellsmentioning

confidence: 99%

The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration

Wu,

Sun,

et al. 2024

Biomedicines

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Mesenchymal stem cells derived‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation

Erkoc‐Biradli,

Erenay,

Ozgun

et al. 2024

Biotechnology Progress

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Amniotic membrane extract (AME) and Wharton's jelly mesenchymal stem cells derived‐exosomes (WJ‐MSC‐Exos) are promising therapeutic solutions explored for their potential in tissue engineering and regenerative medicine, particularly in skin and corneal wound healing applications. AME is an extract form of human amniotic membrane and known to contain a plethora of cytokines and growth factors, making it a highly attractive option for topical applications. Similarly, WJ‐MSC‐Exos have garnered significant interest for their wound healing properties. Although WJ‐MSC‐Exos and AME have been used separately for wound healing research, their combined synergistic effects have not been studied extensively. In this study, we evaluated the effects of both AME and WJ‐MSC‐Exos, individually and together, on the proliferation of corneal keratocytes as well as their ability to promote in vitro cell migration, wound healing, and their impact on cellular morphology. Our findings indicated that the presence of both exosomes (3 × 105 Exo/mL) and AME (50 μg/mL) synergistically enhance the proliferation of corneal keratocytes. Combined use of these solutions (3 × 105 Exo/mL + 50 μg/mL) increased cell proliferation compared to only 50 μg/mL AME treatment on day 3 (**** p < 0.0001). This mixture treatment (3 × 105 Exo/mL + 50 μg/mL) increased wound closure rate compared to isolated WJ‐MSC‐Exo treatment (3 × 105 Exo/mL) (*p < 0.05). Overall, corneal keratocytes treated with AME and WJ‐MSC‐Exo (3 × 105 Exo/mL + 50 μg/mL) mixture resulted in enhanced proliferation and wound healing tendency. Utilization of combined use of AME and WJ‐MSC‐Exo can pave the way for a promising foundation for corneal repair research.

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Frontiers in the Etiology and Treatment of Preterm Premature Rupture of Membrane: From Molecular Mechanisms to Innovative Therapeutic Strategies

Xu,

Yang,

Wen

et al. 2023

Reprod. Sci.

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Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells

Cited by 4 publications

References 63 publications

The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration

The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration

Mesenchymal stem cells derived‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation

Frontiers in the Etiology and Treatment of Preterm Premature Rupture of Membrane: From Molecular Mechanisms to Innovative Therapeutic Strategies

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