Beneficial Effects of Exercise Pretreatment in a Sporadic Alzheimer’s Rat Model

Wu, Chuanjian; Yang, Luodan; Tucker, Donovan; Dong, Yan; Zhu, Ling; Duan, Rui; Liu, Timon Cheng Yi; Zhang, Quanguang

doi:10.1249/mss.0000000000001519

Cited by 75 publications

(105 citation statements)

References 40 publications

(73 reference statements)

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“…To obtain total protein fractions, the homogenization medium was prepared (50 mM 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES), 150 mM NaCl, 12‐b‐glycerophosphate, 3‐dithiothreitol, 2 mM sodium orthovanadate (Na 3 VO 4 ), 1 mM ethylene glycol‐bis(β‐aminoethyl ether)‐N,N,N′,N′‐tetraacetic acid (EGTA), 1 mM NaF, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X‐100, and inhibitors of proteases and enzymes (Thermo Scientific, Rockford, Illinois)) following centrifugation at 15 000 g for 30 minutes. The collection of crude mitochondrial fractions was carried out as previously described . Modified Lowry Protein Assay was performed to determine protein concentrations.…”

Section: Methodsmentioning

confidence: 99%

“…As previously described , proteins (30‐50 μg) were separated on 4% to 20% sodium dodecyl sulfate–polyacrylamide gel (SDS) and transferred onto polyvinylidene fluoride (PVDF) membranes. The PVDF membranes were then blocked with 3% Bovine serum albumin (BSA) in Tris‐Buffered Saline and Tween 20 (TBST) followed by incubation with anti‐cytochrome c (Proteintech, Illinois) antibodies at 4°C for 12 hours.…”

Section: Methodsmentioning

confidence: 99%

“…Caspase‐3 and caspase‐9 activities in cytosolic proteins determined as previously described by our laboratory . Ac‐DEVD‐AMC and Ac‐LEHD‐AMC (AnaSpec, Fremont, California) were used as substrates for detecting caspase‐3 and caspase‐9, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Novel object recognition (NOR) test was applied to measure the effects of PBM pretreatment on recognition memory. Rats have an intrinsic tendency to spend more time on exploring a novel object . To test the long‐term recognition memory, rats were placed in object recognition box with 2 identical objects for 5 minutes at P28.…”

Section: Methodsmentioning

confidence: 99%

“…As previously described , the Barnes Maze (BM) task was applied to test spatial learning and memory. In the current study, a 100‐cm high platform (diameter: 122 cm) with 18 holes around the perimeter was used.…”

Section: Methodsmentioning

confidence: 99%

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Photobiomodulation preconditioning prevents cognitive impairment in a neonatal rat model of hypoxia‐ischemia

Yang

Dong

et al. 2019

Journal of Biophotonics

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Neonatal hypoxia‐ischemia (HI) injury caused by oxygen deprivation is the most common cause of mortality and severe neurologic deficits in neonates. The present work evaluated the preventative effect of photobiomodulation (PBM) preconditioning, and its underlying mechanism of action on brain damage in an HI model in neonatal rats. According to the optimal time response of ATP levels in brain samples removed from normal rats, a PBM preconditioning (PBM‐P) regimen (808 nm CW laser, 1 cm2 spot, 100 mW/cm2, 12 J/cm2) was delivered to the scalp 6 hours before HI. PBM‐P significantly attenuated cognitive impairment, volume shrinkage in the brain, neuron loss, dendritic and synaptic injury after HI. Further mechanistic investigation found that PBM‐P could restore HI‐induced mitochondrial dynamics and inhibit mitochondrial fragmentation, followed by a robust suppression of cytochrome c release, and prevention of neuronal apoptosis by inhibition of caspase activation. Our work suggests that PBM‐P can attenuate HI‐induced brain injury by maintaining mitochondrial dynamics and inhibiting the mitochondrial apoptotic pathway.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Photobiomodulation preconditioning prevents cognitive impairment in a neonatal rat model of hypoxia‐ischemia

Yang

Dong

et al. 2019

Journal of Biophotonics

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Neuroprotective effects of vitamin D in an Alzheimer's disease rat model: Improvement of mitochondrial dysfunction via calcium/calmodulin‐dependent protein kinase kinase 2 activation of Sirtuin1 phosphorylation

Mohanad,

Mohamed,

Aboulhoda

et al. 2023

BioFactors

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Mitochondrial dysfunction is an early event in Alzheimer's disease (AD) pathogenesis. To assess the impact of vitamin D3 (Vit.D) on neurogenesis, we investigated its role in mitigating cognitive impairment and mitochondrial dysfunction through calcium/calmodulin‐dependent protein kinase kinase 2 (CAMKK2)‐mediated phosphorylation of Sirtuin1 (SIRT1) in an aluminum‐chloride‐D‐galactose (AlCl3‐D‐gal)‐induced AD rat model. Rats were distributed into four groups: control, AlCl3 + D‐gal (10 + 60 mg/kg, ip), Vit.D (500 IU/kg, po), and AlCl3 + D‐gal+Vit.D. Novel object recognition (NOR), Morris Water Maze, and passive avoidance (PA) tests were used to measure memory abilities. The hippocampal tissue was used to assess vitamin D3 receptor (VDR) and peroxisome‐proliferator‐activated‐receptor‐γ‐coactivator‐1α (PGC‐1α) expression by quantitative real‐time polymerase chain reaction (qRT‐PCR), CAMKK2, p‐SIRT1, phosphorylated‐AMP‐activated protein kinase (p‐AMPK), dynamin‐related‐protein‐1 (Drp1), and mitofusin‐1 (Mnf1) proteins by western blot and Ca2+ levels, endothelial nitic oxide synthase (eNOS), superoxide dismutase (SOD), amyloid beta (Aβ), and phospho tau (p‐Tau) via enzyme‐linked immunosorbent assay(ELISA) in addition to histological and ultrastructural examination of rat's brain tissue. Vit.D‐attenuated hippocampal injury reversed the cognitive decline and Aβ aggregation, and elevated p‐Tau levels in the AlCl3 + D‐gal‐induced AD rat model. In AlCl3 + D‐gal‐exposed rats, Vit.D induced VDR expression, normalized Ca2+ levels, elevated CAMKK2, p‐AMPK, p‐SIRT1, and PGC‐1α expression. Vit.D reduced Drp1, induced Mnf1, increased mitochondrial membrane potential, preserved mitochondrial structure, restored normal mitochondrial function, and retained normal eNOS level and SOD activity in AlCl3 + D‐gal rats. In conclusion, our findings proved that Vit.D may ameliorate cognitive deficits in AlCl3 + D‐gal‐induced AD by restoring normal mitochondrial function and reducing inflammatory and oxidative stress via CAMKK2‐AMPK/SIRT1 pathway upregulation.

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Photobiomodulation therapy for repeated closed head injury in rats

Yang¹,

et al. 2019

Journal of Biophotonics

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Repeated traumatic brain injury, leads to cumulative neuronal injury and neurological impairments. There are currently no effective treatments to prevent these consequences. Growing interest is building in the use of transcranial photobiomodulation (PBM) therapy to treat traumatic brain injury. Here, we examined PBM in a repeated closed head injury (rCHI) rat model. Rats were administered a total of three closed head injuries, with each injury separated by 5 days. PBM treatment was initiated 2 hours after the first injury and administered daily for a total of 15 days. We found that PBM-treated rCHI rats had a significant reduction in motor ability, anxiety and cognitive deficits compared to CHI group. PBM group showed an increase of synaptic proteins and surviving neurons, along with a reduction in reactive gliosis and neuronal injury. These findings highlight the complexity of gliosis and neuronal injury following rCHI and suggest that PBM may be a viable treatment option to mitigate these effects and their detrimental consequences. K E Y W O R D Sgliosis, neuronal death, photobiomodulation, repeated closed head injury

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Beneficial Effects of Exercise Pretreatment in a Sporadic Alzheimer’s Rat Model

Abstract: Exercise pretraining exerts multifactorial benefits on AD that support its use as a promising new therapeutic option for prevention of neurodegeneration in the elderly and/or AD population.

Cited by 75 publications

References 40 publications

Photobiomodulation preconditioning prevents cognitive impairment in a neonatal rat model of hypoxia‐ischemia

Photobiomodulation preconditioning prevents cognitive impairment in a neonatal rat model of hypoxia‐ischemia

Neuroprotective effects of vitamin D in an Alzheimer's disease rat model: Improvement of mitochondrial dysfunction via calcium/calmodulin‐dependent protein kinase kinase 2 activation of Sirtuin1 phosphorylation

Photobiomodulation therapy for repeated closed head injury in rats

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