Beneficial effects of aminoguanidine on radiotherapy-induced kidney and testis injury

Ekici, Kemal; Temelli, Öztun; Parlakpınar, Hakan; Şamdancı, Emine; Polat, Alaadin; Beytur, A.; Tanbek, Kevser; Ekici, Cemal; Dursun, Ibrahim Hakki

doi:10.1111/and.12500

Cited by 15 publications

(10 citation statements)

References 41 publications

(43 reference statements)

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“…The reduction in antioxidant enzymes is due to the depletion of enzymes during oxidative stress that occurred as a result of irradiation (Khattab et al 2017). In contrast to studies indicating a decrease in GSH in the kidneys (Ekici et al 2016;Ashry et al 2017), a significant increase in GSH levels in the kidneys and ovaries as a result of γ-radiation was observed in this study. GSH is a major non-enzymatic antioxidant involved in the cell defense system against oxidative damage, directly as a free radical scavenger or indirectly by repairing the initial damage to macromolecules being able to maintain protein and nonprotein SH group in a reduced way (Ross 1988;Scibior et al 2008).…”

Section: Discussioncontrasting

confidence: 99%

The effect of curcumin on lipid peroxidation and selected antioxidants in irradiated rats

Özçelik

Erişir

Güler³

et al. 2018

Acta Vet. Brno

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The effect of irradiation on oxidants and antioxidants in selected tissues and a possible protective effect of curcumin on these indices were investigated. A total of 28 rats were divided into 3 groups; group 1 was control; group 2 was the irradiation group, saline was administered intraperiotenally (i.p.) for three days and then, 9 Gy gamma irradiation was applied; group 3 was the irradiation + curcumin group: curcumin was given i.p. for three days at 200 mg/kg body weight and then the same dose of irradiation was applied. A significant increase in malondialdehyde (MDA) was detected in the liver, kidney, and brain tissues of the rats as a result of irradiation (P < 0.01). Glutathione peroxidase (GSH-Px) activity in all the tissues (except for kidneys) decreased (P < 0.01), liver SOD (superoxide dismutase) activity decreased (P < 0.05), and GSH (glutathione) levels in kidney and ovary tissues (P < 0.001) significantly increased. While curcumin administration returned the increased MDA levels in the kidneys and brain in result of irradiation to normal (P < 0.01), it did not return the increased MDA levels in the liver tissue to normal (P < 0.001) despite significantly reducing them. While decreased GSH-Px and SOD activity in the liver in result of irradiation increased with the addition of curcumin (P < 0.05), increased GSH levels in the kidneys and ovaries returned to control levels (P < 0.001). When MDA values were examined, it was found that the addition of curcumin protected the liver, kidneys and brain from the oxidative damage caused by irradiation.

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Section: Discussioncontrasting

confidence: 99%

The effect of curcumin on lipid peroxidation and selected antioxidants in irradiated rats

Özçelik

Erişir

Güler³

et al. 2018

Acta Vet. Brno

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“…The antioxidant enzymes reduced because of the depletion of enzymes during oxidative stress caused by the irradiation (Khattab et al, 2017;Mansour et al, 2008). In contrast to studies indicating the decreased GSH in the kidney (Cosar et al, 2012;Ekici et al, 2016), the present study showed that GSH concentrations significantly increased in the kidney and ovarium as a result of γ-radiation. Being a main intracellular antioxidant, GSH takes part in the defense system of cells against oxidative damage, directly as a free radical scavenger or indirectly by repairing initial damage to macromolecules and could maintain protein and non-protein SH group in a reduced way (Scibior et al, 2008).…”

Section: Discussioncontrasting

confidence: 99%

The Effect of N-Acetylcysteine on Oxidant/Antioxidant Status in Irradiated Rats

Özçelik

Erişir

Güler

et al. 2020

Erciyes Üniversitesi Veteriner Fakültesi Dergisi

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N-Acetylcysteine (NAC) is both a strong antioxidant and a glutathione precursor. The effect of NAC on the oxidant/antioxidant status of some tissues of the irradiated rats was investigated. Twenty one rats were assigned to 3 groups; the control group, the irradiation group, for which physiological saline solution was administered as i.p. for three days and then, 9 Gy gamma irradiation was applied, and 3 rd irradiation + NAC group for which NAC was administered as i.p. for three days as 300 mg/kg body weight and then the same dose of irradiation was applied. Upon irradiation, the increase determined in malondialdehyde (MDA) was significant in the liver, kidney, and brain tissues of the rats (P˂0.05). While glutathione peroxidase (GSH-Px) activity decreased in all of the tissues and superoxide dismutase (SOD) activity only in the liver (P˂0.01), glutathione (GSH) levels significantly increased in the kidney and ovarium tissues (P˂0.001). While NAC administration returned the increased MDA levels in the kidney and brain as a result of irradiation to normal levels (P˂0.05), it was determined that it did not return the increased MDA levels in the liver tissue to the normal level (P˂0.001). While NAC addition led to a significant increase in GSH levels of the liver, heart, spleen, brain and ovarium tissues compared to both control and irradiation groups, it caused a significant decrease in the kidney tissue compared to irradiation group (P˂0.001). As a result of NAC addition, a significant decrease was determined in spleen GSH-Px activity, heart and ovarium SOD activity compared to control and irradiation groups (P˂0.05). It can be asserted that GSH increasing by the addition of NAC is the main antioxidant that has a role in decreasing oxidative stress occurring as a result of irradiation. In the examination of MDA values, it was found that the addition of NAC protected the kidney and brain against the oxidative damage induced by irradiation but NAC addition could remain insufficient for the liver.

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“…Many putative LSC biomarkers have been reported ( Table 2 ) including cell cycle regulators, cytoskeletal proteins, cell adhesion molecules, enzymes, growth factors and their receptors, ATP-binding cassette transporters and differentiation associated markers [ 58 , 59 , 60 ].…”

Section: Putative Biomarkers Of Lscmentioning

confidence: 99%

An Insight into the Difficulties in the Discovery of Specific Biomarkers of Limbal Stem Cells

Guo

Zhang

Jia

et al. 2018

IJMS

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Keeping the integrity and transparency of the cornea is the most important issue to ensure normal vision. There are more than 10 million patients going blind due to the cornea diseases worldwide. One of the effective ways to cure corneal diseases is corneal transplantation. Currently, donations are the main source of corneas for transplantation, but immune rejection and a shortage of donor corneas are still serious problems. Graft rejection could cause transplanted cornea opacity to fail. Therefore, bioengineer-based corneas become a new source for corneal transplantation. Limbal stem cells (LSCs) are located at the basal layer in the epithelial palisades of Vogt, which serve a homeostatic function for the cornea epithelium and repair the damaged cornea. LSC-based transplantation is one of the hot topics currently. Clinical data showed that the ratio of LSCs to total candidate cells for a transplantation has a significant impact on the effectiveness of the transplantation. It indicates that it is very important to accurately identify the LSCs. To date, several putative biomarkers of LSCs have been widely reported, whereas their specificity is controversial. As reported, the identification of LSCs is based on the characteristics of stem cells, such as a nuclear-to-cytoplasm ratio (N/C) ≥ 0.7, label-retaining, and side population (SP) phenotype. Here, we review recently published data to provide an insight into the circumstances in the study of LSC biomarkers. The particularities of limbus anatomy and histochemistry, the limits of the current technology level for LSC isolation, the heterogeneity of LSCs and the influence of enzyme digestion are discussed. Practical approaches are proposed in order to overcome the difficulties in basic and applied research for LSC-specific biomarkers.

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Beneficial effects of aminoguanidine on radiotherapy-induced kidney and testis injury

Cited by 15 publications

References 41 publications

The effect of curcumin on lipid peroxidation and selected antioxidants in irradiated rats

The effect of curcumin on lipid peroxidation and selected antioxidants in irradiated rats

The Effect of N-Acetylcysteine on Oxidant/Antioxidant Status in Irradiated Rats

An Insight into the Difficulties in the Discovery of Specific Biomarkers of Limbal Stem Cells

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