Beneficial effect of sequential chemotherapy treatments of lung cancer patients revealed by calorimetric monitoring of blood plasma proteome denaturation

Koynova, Rumiana; Antonova, Borislava; Sezanova, Boryana; Tenchov, Boris

doi:10.1016/j.tca.2017.11.001

Cited by 16 publications

(11 citation statements)

References 46 publications

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“…Recent studies of sequential chemotherapy also showed that the efficacy of sequential chemotherapy was better than traditional chemotherapy treatment, and the side effects could be acceptable. [49,50] In view of intra-tumor heterogeneity of EC, sequential chemotherapy may have certain advantages in the treatment of EC, and whether it could become the clinical application chemotherapy regimen for EC needs further verification.…”

Section: Discussionmentioning

confidence: 99%

Intra-tumor heterogeneity for endometrial cancer and its clinical significance

Yin

Zhao

et al. 2019

Chinese Medical Journal

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Background: Management of tumors has become more complex owing to tumor heterogeneity. Fewer studies have been performed on intra-tumor heterogeneity of endometrial cancer (EC) until now. Therefore, it is of great clinical value to explore the intra-tumor heterogeneity of EC based on clinical features and gene expression profiles. Methods: A total of 1688 patients with EC were screened and 114 patients were finally selected, including specimens from 84 patients with primary EC without relapse (PE) and the paired metastases (P-M) specimens, as well as specimens from 30 patients with primary EC with relapse (RPE) and the paired relapsed EC (P-RE) specimens. Microarray and RNA-seq were used to detect gene expression of EC samples. Clinicopathological characteristics and molecular data were compared between PE and P-M groups and between RPE and P-RE groups to explore the intra-tumor heterogeneity of EC. Results: The clinical intra-tumor spatial heterogeneity of pathological type, grade, ER status, and PR status between PE and P-M were 17.9%, 13.1%, 28.6%, and 28.6%, respectively. The clinical intra-tumor spatiotemporal heterogeneity of pathological type, grade, ER status, and PR status between RPE and P-RE were 16.7%, 33.3%, 25.0%, and 37.5%, respectively. Cluster analysis sorts EC samples based on progression type of lesion and their pathological type. There were differentially expressed genes between PE and P-M and between RPE and P-RE, of which gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis were mainly enriched in cell proliferation, the p53 signaling pathway, etc. Conclusions: Clinical and molecular data showed that there was spatiotemporal heterogeneity in intra-tumor of EC, which may add to the complexity of diagnosis and therapeutics for EC. Considering the intra-tumor heterogeneity, sequential chemotherapy and precision medicine may be a more suitable treatment plan for EC.

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Section: Discussionmentioning

confidence: 99%

Intra-tumor heterogeneity for endometrial cancer and its clinical significance

Yin

Zhao

et al. 2019

Chinese Medical Journal

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“…The polymeric solution was electrospun with an applied voltage of 15 kV, onto glass coverslips attached to a metallic target to create fine fibers. The resulting flat sheets were cross-linked in 25% glutaraldehyde (Sigma-Aldrich, St. Louis, MO, USA) in vapor phase for 24 h. The glutaraldehyde was evaporated in a convection oven overnight at 42 °C, which is a temperature below the denaturation point of gelatin and fibrinogen [ 36 , 37 ]. Additionally, the scaffolds were rinsed with deionized water to remove any cross-linker residues and uncross-linked gelatin [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Surface Modification of Electrospun Scaffolds for Endothelialization of Tissue-Engineered Vascular Grafts Using Human Cord Blood-Derived Endothelial Cells

Ardila

Liou

Maestas

et al. 2019

JCM

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Tissue engineering has gained attention as an alternative approach for developing small diameter tissue-engineered vascular grafts intended for bypass surgery, as an option to treat coronary heart disease. To promote the formation of a healthy endothelial cell monolayer in the lumen of the graft, polycaprolactone/gelatin/fibrinogen scaffolds were developed, and the surface was modified using thermoforming and coating with collagen IV and fibronectin. Human cord blood-derived endothelial cells (hCB-ECs) were seeded onto the scaffolds and the important characteristics of a healthy endothelial cell layer were evaluated under static conditions using human umbilical vein endothelial cells as a control. We found that polycaprolactone/gelatin/fibrinogen scaffolds that were thermoformed and coated are the most suitable for endothelial cell growth. hCB-ECs can proliferate, produce endothelial nitric oxide synthase, respond to interleukin 1 beta, and reduce platelet deposition.

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“…The position and amplitude of the resolved transitions as well as the weighted average center (T FM ) of plasma/sera thermograms were shown to be rather constant for serum derived from healthy individuals [ 1 , 2 , 4 ]. These thermodynamic parameters were shown to change significantly for a number of diseases [ 1 , 4 , 9 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ], and especially for various types of cancer—breast [ 20 , 21 , 22 , 23 ], pancreatic [ 24 ], cervical [ 25 , 26 , 27 ], lung [ 28 , 29 , 30 , 31 , 32 ], brain [ 6 , 31 , 33 ], colorectal [ 18 ], gastric adenocarcinoma [ 34 ], melanoma [ 35 , 36 , 37 ], multiple myeloma (MM) [ 5 , 38 , 39 , 40 , 41 , 42 , 43 ], chronic lymphocytic leukemia and acute myeloid leukemia [ 44 ]—and can therefore be used to stratify DSC data into different disease-related groups.…”

Section: Application Of Differential Scanning Calorimetry For Plasma/...mentioning

confidence: 99%

Calorimetric Markers for Detection and Monitoring of Multiple Myeloma

Krumova

Todinova

Taneva

2022

Cancers

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This review summarizes data obtained thus far on the application of differential scanning calorimetry (DSC) for the analysis of blood sera from patients diagnosed with multiple myeloma (MM) with the secretion of the most common isotypes of monoclonal proteins (M-proteins), free light chains (FLC) and non-secretory MM, as well as Waldenström macroglobulinemia and the premalignant state monoclonal gammopathy of undetermined significance. The heterogeneous nature of MM is reflected in the thermal stability profiles of the blood serum proteome of MM patients found to depend on both the level and the isotype of the secreted M-proteins or FLC. Common calorimetric markers feature the vast majority of the different myeloma types, i.e., stabilization of the major serum proteins and decrease in the albumin/globulin heat capacity ratio. A unique calorimetric fingerprint of FLC molecules forming amorphous aggregates is the low-temperature transition centered at 57 °C for a calorimetric set of FLC MM and at 46–47 °C for a single FLC MM case for which larger aggregates were formed. The calorimetric assay proved particularly advantageous for non-secretory MM and is thus a suitable tool for monitoring such patients during treatment courses. Thus, DSC provides a promising blood-based approach as a complementary tool for MM detection and monitoring.

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Beneficial effect of sequential chemotherapy treatments of lung cancer patients revealed by calorimetric monitoring of blood plasma proteome denaturation

Cited by 16 publications

References 46 publications

Intra-tumor heterogeneity for endometrial cancer and its clinical significance

Intra-tumor heterogeneity for endometrial cancer and its clinical significance

Surface Modification of Electrospun Scaffolds for Endothelialization of Tissue-Engineered Vascular Grafts Using Human Cord Blood-Derived Endothelial Cells

Calorimetric Markers for Detection and Monitoring of Multiple Myeloma

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