Surface Modification of Electrospun Scaffolds for Endothelialization of Tissue-Engineered Vascular Grafts Using Human Cord Blood-Derived Endothelial Cells

Ardila, Diana Catalina; Liou, Jr-Jiun; Maestas, David R.; Slepian, Marvin J.; Badowski, Michael; Wagner, William R.; Harris, David T.; Geest, Jonathan P. Vande

doi:10.3390/jcm8020185

Cited by 33 publications

(15 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our constructs showed pore sizes between 0.08 ± 0.01 µm 2 and 0.12 ± 0.05 µm 2 , which lies in the range of a native vessel. Several studies have already described that FN improves the endothelialization of vascular grafts [19,48,49,51]. In our study, we observed a fibrous-like structure of the coated FN (Figure 3b).…”

Section: Discussionsupporting

confidence: 73%

“…It is of interest with regard to endothelialization, as it plays a pivotal role in wound healing [45,46]. Several studies described the coating of FN in combination with collagens type I [47] and type IV [48], with fibrinogen and tropoelastin [49], hepatocyte growth factor [50], heparin, Cells 2020, 9,778 3 of 29 and VEGF [51] and with SDF-1α [19] to improve reendothelialization. However, it has never been used in combination with DCN before.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

Daum

Visser

Wild

et al. 2020

Cells

View full text Add to dashboard Cite

Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 ± 0.6 MPa, burst pressure: 3326 ± 78 mmHg), which were biofunctionalized with fibronectin (FN) and decorin (DCN). Neither uncoated nor biofunctionalized TPCU scaffolds induced major adverse immune responses except for minor signs of polymorph nuclear cell activation. The in vivo endothelial progenitor cell homing potential of the biofunctionalized scaffolds was simulated in vitro by attracting endothelial colony-forming cells (ECFCs). Although DCN coating did attract ECFCs in combination with FN (FN + DCN), DCN-coated TPCU scaffolds showed a cell-repellent effect in the absence of FN. In a tissue-engineering approach, the electrospun and biofunctionalized tubular grafts were cultured with primary-isolated vascular endothelial cells in a custom-made bioreactor under dynamic conditions with the aim to engineer an advanced therapy medicinal product. Both FN and FN + DCN functionalization supported the formation of a confluent and functional endothelial layer.

show abstract

Section: Discussionsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

Daum

Visser

Wild

et al. 2020

Cells

View full text Add to dashboard Cite

show abstract

“…In addition, as another important ingredient of ECM, FN play a key role in promoting cell adhesion, proliferation and signal transduction. Ardila et al believed that the FN is an active ingredient in stimulating the formation of ECs lumen, while other ECM molecules will only provide the necessary ingredients for the environment [26]. Therefore, in this study we used stem cell-derived cell sheets that provided the native ECM components to study the interaction between ECM microenvironment and ECs.…”

Section: Discussionmentioning

confidence: 99%

Regulation of Matrix Metalloproteinase in Vascularization of Endothelial Cells On Extracellular Matrix Secreted By Mesenchymal Stem Cell Sheet

Ren

Yang

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: We have previously developed a prevascularized platform by seeding endothelial cells on the mesenchymal stem cell sheet and the technology provides a promising vascularization potential for wide applications in tissue engineering. However, the mechanism behind the interactions between stem cell sheet-derived matrix and endothelial cells (ECs) are still unknown. The purpose of this study is to investigate the role of MMPs in angiogenesis process in vitro.Methods: Human bone marrow-derived mesenchymal stem cells (hBMSCs) were cultured continually to form a thin cell sheet, and then green fluorescent protein (GFP)-labeled human umbilical vein endothelial cells (HUVECs) were seeded on the cell sheet to form a prevascularized system. Fibronectin, collagen I and MMPs immunofluorescence staining were used to observe their interaction changes. After that, HUVECs and hBMSCs were sorted by flow cytometry and RT-PCR was performed to quantify related gene expression of ECs and stem cells. In order to further explore the possible mechanism, the broad-spectrum MMPs inhibitor GM6001 was added into the medium to observe the effects of vascular network formation. Results: The immunofluorescence staining results showed that HUVECs secreted MMP-2 and membrane-type matrix metalloproteinase-1 (MT1-MMP) in the early stage of angiogenesis. MMPs degraded and remodeled the ECM to create space for vascular formation. HUVECs also promoted hBMSCs to secrete tissue inhibitors of metalloproteinase-2 (TIMP-2), which can stabilize blood vessels by avoiding excessive vascularization or vascular degradation in the later stage. Thus, HUVECs/hBMSCs co-culture group were beneficial for angiogenesis than the other two control groups. With the addition of the broad-spectrum matrix metalloproteinase inhibitor GM6001, RT-PCR results showed that the formation of EC lumen was blocked, while the mean fluorescence density of fibronectin and collagen I were increased.Conclusions: our study suggests that the MMP-2, MT1-MMP and TIMP-2 can synergistically regulate the angiogenesis. In addition, HUVECs and hBMSCs interact with each other in the process of vascular formation, and jointly regulate and promote the construction of lumen. These results also indicate a promising potential using the hBMSCs sheet as a platform for broad applications in engineering vascularized tissues.

show abstract

“…TGF-β1 could promote the differentiation of MSC to the myogenic lineage and enhance mechanical properties of the vascular constructs. The results revealed that the conjugation of TGF-β1 in a 3D fibrin matrix induced the activated Smad2 signal in fibrin-embedded cells for several days, leading to an increased vascular contractility [31,32].…”

Section: Biomimetic Materials For Tissue Regenerationmentioning

confidence: 99%

Functional Protein-Based Bioinspired Nanomaterials: From Coupled Proteins, Synthetic Approaches, Nanostructures to Applications

Zhang

Wang

2019

IJMS

View full text Add to dashboard Cite

Protein-based bioinspired nanomaterials (PBNs) combines the advantage of the size, shape, and surface chemistry of nanomaterials, the morphology and functions of natural materials, and the physical and chemical properties of various proteins. Recently, there are many exciting developments on biomimetic nanomaterials using proteins for different applications including, tissue engineering, drug delivery, diagnosis and therapy, smart materials and structures, and water collection and separation. Protein-based biomaterials with high biocompatibility and biodegradability could be modified to obtain the healing effects of natural organisms after injury by mimicking the extracellular matrix. For cancer and other diseases that are difficult to cure now, new therapeutic methods involving different kinds of biomaterials are studied. The nanomaterials with surface modification, which can achieve high drug loading, can be used as drug carriers to enhance target and trigger deliveries. For environment protection and the sustainability of the world, protein-based nanomaterials are also applied for water treatment. A wide range of contaminants from natural water source, such as organic dyes, oil substances, and multiple heavy ions, could be absorbed by protein-based nanomaterials. This review summarizes the formation and application of functional PBNs, and the details of their nanostructures, the proteins involved, and the synthetic approaches are addressed.

show abstract

Surface Modification of Electrospun Scaffolds for Endothelialization of Tissue-Engineered Vascular Grafts Using Human Cord Blood-Derived Endothelial Cells

Cited by 33 publications

References 103 publications

Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

Regulation of Matrix Metalloproteinase in Vascularization of Endothelial Cells On Extracellular Matrix Secreted By Mesenchymal Stem Cell Sheet

Functional Protein-Based Bioinspired Nanomaterials: From Coupled Proteins, Synthetic Approaches, Nanostructures to Applications

Contact Info

Product

Resources

About