Beneficial Effect of Jojoba Seed Extracts on Hyperglycemia-Induced Oxidative Stress in RINm5f Beta Cells

Belhadj, Sahla; Hentati, Olfa; Hamdaoui, Ghaïth; Fakhreddine, Khaskhoussi; Maillard, Élisa; Dal, Stéphanie; Sigrist, S.

doi:10.3390/nu10030384

Cited by 27 publications

(15 citation statements)

References 85 publications

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“…In a previous study, we have already demonstrated in vitro the protective effect of Jojoba seed extracts against hyperglycemia-induced oxidative stress through the modulation of RINm5f beta cell cytotoxicity, generation of ROS, insulin release, caspase-3, activation, pro-oxidant and antioxidant defense, and status of the cells. Using this elegant in vivo insulin resistance model, our purpose is to demonstrate the efficiency of jojoba seeds [18].…”

Section: Discussionmentioning

confidence: 99%

“…In a recent study [18], we have demonstrated that extraction of jojoba seeds by distilled water endorses different classes of polyphenols such as hydroxybenzoic acid, flavonoids, anthocyanins and hydroxycinnamic acids. For the first time, this study showed hypoglycemic and antioxidant effects, of simmondsin and aqueous extract, on Rin m5f cells.…”

Section: Introductionmentioning

confidence: 95%

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Anorexic and metabolic effect of jojoba: potential treatment against metabolic syndrome and hepatic complications

Belhadj

Dal

Fakhreddine³

et al. 2020

Nutr Metab (Lond)

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Background: Evaluation of the action of various traditional plants to treat metabolic syndrome are strongly studied. In our study, we investigated the effect of the Tunisian jojoba seed on a metabolic syndrome induced in rat by the High Fat diet and High Fructose (HFHF) and its renal and hepatic complications. Methods: The rats were fed with HFHF or Normal Diet (ND) for a period of 8 weeks. After that, a switch from HFHF to ND or Normal Diet Jojoba (NDJ),(jojoba diet approach) or High Fat and High Fructose and Jojoba diet (HFHFJ) (nutraceutical approach) has been done. Metabolic disorder was evaluated by measuring the fasting body weight, glycemia and C-peptide and leptin. Oxidative stress parameters like ThioBarbituric Acid Reactive Substances (TBARS) and Total Antioxidant Capacity (TAOC) were analyzed in the plasma and renal and hepatic function were determined by the measure of creatinine and alanine transferase (ALT) respectively. Histological analysis was performed on the liver, kidney and pancreas. Results: HFHF diet exhibited characteristics of metabolic syndrome presented by insulin resistance, hyperinsulinemia, hyperleptinemia, fat mass with hepatic steatosis and renal disorder. HFHF diet was associated with oxidative stress (OS) presented by an increase in TBARS and a decrease in TAOC. Adding jojoba seeds to HFHF rat group diet induced a decrease in body weight, fat mass (58 and 41%), insulin resistance (59 and 56%), oxidative stress (60 and 41%), liver steatosis (from a score = 3 to a score = 0) and renal complications (25 and 42%). This effect was emphasized with diet approach. Conclusion: The results demonstrated the beneficial effect of jojoba against metabolic syndrome and oxidative stress, suggesting that jojoba could be used in the prevention and treatment of metabolic syndrome.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Anorexic and metabolic effect of jojoba: potential treatment against metabolic syndrome and hepatic complications

Belhadj

Dal

Fakhreddine³

et al. 2020

Nutr Metab (Lond)

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“…Extracts from jojoba seed, containing a wide range of phenolic compounds, demonstrated an active free radical scavenging ability leading to improved β-cell protection [83]. A study using rat cells (RINm5f) demonstrated that jojoba seed crude extracts significantly reduced ROS levels by 69% when compared to simmondsin, the major phenolic compound found in jojoba [78]. This pattern was also reflected in caspase activation, antioxidant activity and pro-oxidant signalling pathways, where the crude extracts significantly increased the expression of Nrf2 while simmondsin displayed no effect.…”

Section: Polyphenols and Gene Modulations On β-Cell Dysfunctionmentioning

confidence: 99%

“…Nrf2 plays a key role in antioxidant defence, while p22phox activates NADPH oxidase, generating excessive ROS [84,85]. The regulation of both genes by jojoba seed extracts preserved β-cell function demonstrated by increased insulin secretion [78].…”

Section: Polyphenols and Gene Modulations On β-Cell Dysfunctionmentioning

confidence: 99%

Dietary Polyphenols and Gene Expression in Molecular Pathways Associated with Type 2 Diabetes Mellitus: A Review

Kang

Francis

Hill

et al. 2019

IJMS

108

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Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder with various contributing factors including genetics, epigenetics, environment and lifestyle such as diet. The hallmarks of T2DM are insulin deficiency (also referred to as β-cell dysfunction) and insulin resistance. Robust evidence suggests that the major mechanism driving impaired β-cell function and insulin signalling is through the action of intracellular reactive oxygen species (ROS)-induced stress. Chronic high blood glucose (hyperglycaemia) and hyperlipidaemia appear to be the primary activators of these pathways. Reactive oxygen species can disrupt intracellular signalling pathways, thereby dysregulating the expression of genes associated with insulin secretion and signalling. Plant-based diets, containing phenolic compounds, have been shown to exhibit remedial benefits by ameliorating insulin secretion and insulin resistance. The literature also provides evidence that polyphenol-rich diets can modulate the expression of genes involved in insulin secretion, insulin signalling, and liver gluconeogenesis pathways. However, whether various polyphenols and phenolic compounds can target specific cellular signalling pathways involved in the pathogenesis of T2DM has not been elucidated. This review aims to evaluate the modulating effects of various polyphenols and phenolic compounds on genes involved in cellular signalling pathways (both in vitro and in vivo from human, animal and cell models) leading to the pathogenesis of T2DM.

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“…The effect of extracts was evaluated on insulin secreting cell lines according to the method described by Auberval and slightly modified by Belhadj et al [29,30]. Briefly, a rat insulinoma cell line (RINm5f beta) was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).…”

Section: Cell Viability Testmentioning

confidence: 99%

Influence of the physicochemical parameters of solvents in the extraction of bioactive compounds from Parinari macrophylla Sabine (Chrysobalanaceae)

et al. 2018

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The extraction of bioactive compounds from medicinal plants requires methods which are as diverse as the chemical nature of the compounds themselves. In this study, a 96-well microplate was used where solvent mixtures spanning wide ranges of selectivity and polarity were tested with the objective of extracting a broad range bioactive compounds from plant material. Microplate wells were filled with plant material and the solvents and their mixtures were added. The obtained extracts were assessed in terms of their total antioxidant activity, oxygen radical absorbance capacity and effects on cell viability. An aqueous extract, generally used by traditional therapists, was also included in the study. The results showed that the extracts using methanol with acetic acid (0.1%, v:v), chloroform/ethanol, butanol/DMF, butanol/acetonitrile, ethylene glycol with acetic acid (0.1%, v:v), MTBE/DMSO, ethylene glycol, pentane/ethanol (v:v), ethanol, DMF, DMF with acetic acid (0.1%, v:v), DMSO, DMSO with acetic acid (0.1%, v:v) and THF had a higher antioxidant activity than the aqueous extract. Extracts with greater antioxidant activity than the aqueous extract were obtained largely from solvent mixtures with the exception of ethanol, DMF, DMSO and THF. The antioxidant activity obtained in TEAC varied between 1474.1±4.4 and 3183.0±16.0 μmol TE/g dry extract respectively for aqueous and THF extracts; in ORAC between 1727.7±8.4 and 2683.5±11.7 μmol TE/g dry extract for aqueous and DMSO acetic acid 1%, respectively, with mean ±SEM. In TEAC the THF extract had the highest antioxidant potential with 3183.0±16.0 μmol TE / g dry extract. The DMSO acetic acid (0.1%, v:v) extract had the highest antioxidant potential in ORAC with 2683.5±11.7 μmol TE / g dry extract. Cell viability test using β-pancreatic cells showed that only the acidified methanol extract was toxic after one hour of incubation. After 24 hours, cell viability was less than 70% for extracts using butanol/acetonitrile, MTBE/DMF, acidified methanol, pentane/ethanol and acidified DMF.

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Beneficial Effect of Jojoba Seed Extracts on Hyperglycemia-Induced Oxidative Stress in RINm5f Beta Cells

Cited by 27 publications

References 85 publications

Anorexic and metabolic effect of jojoba: potential treatment against metabolic syndrome and hepatic complications

Anorexic and metabolic effect of jojoba: potential treatment against metabolic syndrome and hepatic complications

Dietary Polyphenols and Gene Expression in Molecular Pathways Associated with Type 2 Diabetes Mellitus: A Review

Influence of the physicochemical parameters of solvents in the extraction of bioactive compounds from Parinari macrophylla Sabine (Chrysobalanaceae)

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