Beneficial effect of iloprost® on impaired colonic anastomotic healing induced by intraperitoneal 5-fluorouracil infusion

Bostanoğlu, Settar; Dinçer, Sibel; Keskïn, Ali; Bostanoğlu, Akın; Dursun, A; Serim, C

doi:10.1007/bf02235275

Cited by 26 publications

(35 citation statements)

References 22 publications

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“…The dosage was calculated by reviewing literature about the amount of iloprost that was effective on the ischemic tissue and the amount was given as a single dose after the surgery iloprost was administrated in the early period after the laparatomy to the rats in groups 1 and 3 12,13 . …”

Section: Iloprost Therapymentioning

confidence: 99%

Iloprost reduces colonic injury in ischemic colitis in rats

Karatepe¹,

Cakir²,

Ünal³

et al. 2011

Acta Cir. Bras.

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Purpose: Evaluate the effects of iloprost administration in the early period of ischemic colitis and the mechanism that how these effects develop. Methods: Thirty two Wistar albino female rats with an average weight of 220g were divided into four groups of eight rats. In group 1 the rats were given iloprost and sacrificed after 24 hours and in group 2 they were sacrificed after 24 hours without any iloprost. The rats in group 3 were administrated iloprost and sacrificed after 72 hours and in group 4 they were sacrificed at 72 th hour without iloprost. The differences between the groups as tissue damage, vascularization or apoptosis were assessed statistically. Results: Oxidative damage and apoptosis were less pronounced and vascularization was better developed in rats that were given iloprost and sacrificed at 24 th hour later in contrast to the rats that were not treated with iloprost. But there was no statistical difference among the groups at 72 th hour. Conclusion: Iloprost inhibited leucocyte infiltration, decreased proinflammatory cytokines and enhanced angiogenesis so that the oxidative stress and inflammatory response decreased resulting in lesser tissue damage. Key words: Colitis, Ischemic. Iloprost. Epoprostenol. Arachidonic Acid. Prostaglandins. Rats. RESUMOObjetivo: Avaliar os efeitos da administração de iloprosta no período precoce da colite isquêmica e o mecanismo da evolução destes efeitos. Métodos: Trinta e dois ratos Wistar fêmeas em torno de 220g foram distribuídos em quatro grupos de oito ratos. No grupo 1 administração de iloprosta e sacrificados após 24 horas; no grupo 2 foram sacrificados após 24 horas sem iloprosta; no grupo 3 foi administrado iloprosta e sacrificados após 72 horas; no grupo 4 foram sacrificados após 72 horas sem Iloprosta. As diferenças entre os grupos no referente a dano tecidual. vascularização ou apoptose foi apurada estatisticamente. Resultados: Dano oxidativo e apoptose foram menos acentuados e a vascularização foi melhor nos ratos que receberam iloprosta e sacrificados após 24 horas em contraste com os ratos que não receberam iloprosta. Porém, não houve diferença estatisticamente significante entre os grupos de 72 horas. Conclusão: Iloprosta inibe infiltração leucocitária, diminui a ação inflamatória de citoquinas e estimula angiogênese resultando em menor dano tecidual. Descritores: Colite Isquêmica. Iloprosta. Epoprostenol. Ácido Araquidônico. Prostaglandinas. Ratos.

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Section: Iloprost Therapymentioning

confidence: 99%

Iloprost reduces colonic injury in ischemic colitis in rats

Karatepe¹,

Cakir²,

Ünal³

et al. 2011

Acta Cir. Bras.

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“…To assess the strength of colonic anastomoses, the most reliable method is considered to be the measurement of their bursting pressure (28,29). In a study in rats, Bostanoglou et al (30) have mentioned that the administration of iloprost caused no statistically significant increase in the bursting pressure of the colon anastomoses. In our study, through the administration of iloprost in group 4, statistically significantly increased values in the bursting pressure of the anastomoses compared with group 2 (P <0.001) were observed.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Iloprost in the Healing of Colonic Anastomosis in Rats under Chemotherapy with Irinotecan

Arapoglou¹,

Kambaroudis²,

Grivas³

et al. 2017

chr

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RezumatScop: Am investigat posibilele efecte pozitive ale iloprostului în vindecarea anastomozelor colonice, la şobolani sub chimioterapie intraperitoneală cu irinotecan. Metodă: Patruzeci de şobolani masculi Wistar au fost împărţiţi în patru grupuri. În fiecare caz s-a practicat o colectomie parţială segmentară cu anastomoză termino-terminală. Ulterior li s-a administrat intraperitoneal, ser fiziologic (grupul 1), irinotecan (grupul 2), iloprost (grupul 3), irinotecan şi iloprost (grupul 4). Etapele următoare au constat în: autopsie, examinare macroscopică şi măsurarea presiunii de ruptură anastomotică. Un fragment al anastomozei a fost trimis pentru examen histologic şi pentru determinarea nivelurilor de hidroxiprolină. Rezultate: Frecvenţa fistulei anastomotice, cât şi a formării de aderenţe intraperitoneale au fost semnificativ crescute în grupul 2 comparativ cu celelalte grupuri. Presiunea de liză a anastomozei a fost semnificativ mai scăzută în grupul 2, comparativ cu toate celelalte grupuri; de asemenea, acest indicativ a fost semnificativ crescut în grupul 4 comparativ cu grupul 2. Gradul de leucocitoză, numărul de fibroblasti, nivelele de neocollagen şi hidroxiprolină din grupul 4 au fost semnificativ crescute comparativ cu grupul

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“…Late anti-NGF group (Group LA-NGF, n=9) was given 500 µg/kg anti-NGF antibody immediately after (at the 10 th hour) sepsis became apparent following LPS injection. 11 Subjects which did not show sepsis symptoms within 12 hours were excluded from the study. They were replaced with new ones to reach a total number of 33 subjects.…”

Section: Methodsmentioning

confidence: 99%

Effects of Anti-NGF on Apoptosis in Rats with Experimentally Induced Sepsis Model

Bayar¹,

Özer²,

Beştaş³

et al. 2010

Turkiye Klinikleri J Med Sci

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A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: : This study aimed to investigate the effects of anti-NGF administration on apoptosis in rats with experimentally induced sepsis model, and to examine the possible role of nerve growth factor (NGF) in pathogenesis and treatment of sepsis. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Thirty three Wistar Albino rats were randomly allocated into four groups. After sparing the control group, the remaining three groups were given intraperitoneal E. coli lipopolysaccaride to induce sepsis. Early anti-NGF group (Group EA-NGF, n=9) was injected with intraperitoneal anti-NGF one hour before LPS injection, and late anti-NGF group (Group LA-NGF, n=9) after sepsis was induced. Baselines, 2, 12, 24, and 72nd hours were analyzed to determine NGF levels. The rats were sacrificed on the 3rd day, and tissue samples separated from the small intestines, liver and lung. HE, bcl-2 and bax staining were used to evaluate apoptosis. R Re es su ul lt ts s: : Apoptosis increased most markedly in the intestine tissue, as well as in the liver and lung tissue, and this increase was most evident in the early anti-NGF group. Bcl-2 staining in the subjects was less apparent while bax staining was more intensely detected in the sepsis groups, with a significant difference between the sepsis and early anti-NGF groups. Bax staining in the EA-NGF group was more clearly observed than that in LA-NGF group. NGF levels were found to increase in the 24th hour in sepsis group (Group S) and decrease in the second hour in Group EA-NGF. C Co on nc cl lu us si io on n: : It can be concluded that anti-NGF administration in the early period of sepsis increases apoptosis at least to the same extent with sepsis, and it decreases apoptosis when administered in the later period of sepsis.K Ke ey y W Wo or rd ds s: : Sepsis; 7S nerve growth factor protein, mouse; apoptosis Ö ÖZ ZE ET T A Am ma aç ç: : Bu ça lış ma da de ney sel sep sis mo de li oluş tu ru lan rat lar da, an ti-NGF uy gu lan ma sı nın apo pi toz üze ri ne et ki le ri ni de ğer len di re rek Ner ve growth fak tö rün (NGF) sep sis pa to ge nez ve teda vi sin de ki ola sı ro lü nün araş tı rıl ma sı amaç lan dı. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Otuz üç ta ne Wis tar-Al bino cin si rat rast ge le dört gru ba ay rıl dı. Kon trol gru bu ay rıl dık tan son ra di ğer üç gru ba E. co li lipopolisakkaridi in tra pe ri to ne al ola rak uy gu la na rak sep sis kli ni ği oluş tu rul du. Fark lı ola rak er ken an ti-NGF grup (Grup EA-NGF, n=9) LPS uy gu lan ma dan 1 sa at ön ce ve geç an ti-NGF grup (Grup LA-NGF, n=9 )'ye sep sis kli ni ği oluş tu rul duk tan son ra an ti-NGF in tra pe ri to ne al ola rak uy gu lan dı. De nek ler den ba zal, 2, 12, 24 ve 72. sa at ler de kan ör nek le ri alı na rak se rum NGF dü zey le ri öl çül dü. De ne yin üçüncü gü nün de sak ri fi ye edi len rat la rın in ce bar sak, ka ra ci ğer ve ak ci ğer do ku la rı ay rı -la rak he ma tok si len eo zin (H/E) ve im mu no his to kim ya sal ola ...

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Beneficial effect of iloprost® on impaired colonic anastomotic healing induced by intraperitoneal 5-fluorouracil infusion

Abstract: Our study showed a positive effect of Iloprost on the healing of colon anastomosis and, more importantly, if wound-healing is impaired by a chemotherapeutic agent, Iloprost counteracts and reverses the effect. [Key words: 5-Fluorouracil; Healing of colon anastomoses; Iloprost

Cited by 26 publications

References 22 publications

Iloprost reduces colonic injury in ischemic colitis in rats

Iloprost reduces colonic injury in ischemic colitis in rats

The Effect of Iloprost in the Healing of Colonic Anastomosis in Rats under Chemotherapy with Irinotecan

Effects of Anti-NGF on Apoptosis in Rats with Experimentally Induced Sepsis Model

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