Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer

Lu, Haoran; Xiao, Kaijie; Tian, Zhixin

doi:10.1007/s10719-021-09994-8

Cited by 14 publications

(10 citation statements)

References 116 publications

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“…LC-MS analysis identified 20,038 intact N-glycopeptides, with 38 up-regulated and 14 down-regulated in cancer compared to control. These peptides correspond to proteins involved in several processes, including transporter and catalytic activity and binding (Lu et al, 2021). Another work studied two patient-derived xenograft mouse models to compare the effects of cancer differentiation on both the N-linked and O-linked glycomes.…”

Section: Pancreatic Cancer Biomarker Discoverymentioning

confidence: 99%

Recent Advances in Mass Spectrometry-Based Glycomic and Glycoproteomic Studies of Pancreatic Diseases

Tabang

Ford

2021

Front. Chem.

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Modification of proteins by glycans plays a crucial role in mediating biological functions in both healthy and diseased states. Mass spectrometry (MS) has emerged as the most powerful tool for glycomic and glycoproteomic analyses advancing knowledge of many diseases. Such diseases include those of the pancreas which affect millions of people each year. In this review, recent advances in pancreatic disease research facilitated by MS-based glycomic and glycoproteomic studies will be examined with a focus on diabetes and pancreatic cancer. The last decade, and especially the last five years, has witnessed developments in both discovering new glycan or glycoprotein biomarkers and analyzing the links between glycans and disease pathology through MS-based studies. The strength of MS lies in the specificity and sensitivity of liquid chromatography-electrospray ionization MS for measuring a wide range of biomolecules from limited sample amounts from many sample types, greatly enhancing and accelerating the biomarker discovery process. Furthermore, imaging MS of glycans enabled by matrix-assisted laser desorption/ionization has proven useful in complementing histology and immunohistochemistry to monitor pancreatic disease progression. Advances in biological understanding and analytical techniques, as well as challenges and future directions for the field, will be discussed.

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Section: Pancreatic Cancer Biomarker Discoverymentioning

confidence: 99%

Recent Advances in Mass Spectrometry-Based Glycomic and Glycoproteomic Studies of Pancreatic Diseases

Tabang

Ford

2021

Front. Chem.

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“…GPSeeker, 24 an intact Nglycopeptide search engine, was previously successfully used for structure-specific N-glycan characterization of recombinant COVID-19 S-protein, 25 as well as complex N-glycoproteomes from various sample types, e.g., urine, 26 cell, 27 and tissue. 28 Shen et al 26 identified 2986 intact N-glycopeptides including 146 confirmed N-glycan structures in human urine. In the study of differential N-glycosylation of MCF-7/ADR cancer stem cells, Wang et al 27 identified 546 N-glycopeptides with structurediagnostic ions.…”

Section: Introductionmentioning

confidence: 99%

“…In the study of differential N-glycosylation of MCF-7/ADR cancer stem cells, Wang et al 27 identified 546 N-glycopeptides with structurediagnostic ions. Most recently, Lu 28 et al 28 reported 1152 intact N-glycopeptide-modified structure isomers with structurediagnostic ions in pancreatic cancer tissues.…”

Section: Introductionmentioning

confidence: 99%

“…Here, we report our structure-specific N- glycoproteomics characterization of RM 8671 with GPSeeker search of structure-diagnostic fragment ions from tandem mass spectra to distinguish different monosaccharide sequence structures from the same monosaccharide composition: 59 intact N- glycopeptides with distinct N- glycan structures including 16 pairs of structure isomers are identified. GPSeeker, an intact N- glycopeptide search engine, was previously successfully used for structure-specific N- glycan characterization of recombinant COVID-19 S-protein, as well as complex N- glycoproteomes from various sample types, e.g., urine, cell, and tissue . Shen et al identified 2986 intact N- glycopeptides including 146 confirmed N- glycan structures in human urine.…”

Section: Introductionmentioning

confidence: 99%

“…identified 546 N- glycopeptides with structure-diagnostic ions. Most recently, Lu et al reported 1152 intact N- glycopeptide-modified structure isomers with structure-diagnostic ions in pancreatic cancer tissues.…”

Section: Introductionmentioning

confidence: 99%

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Structure-Specific N-Glycoproteomics Characterization of NIST Monoclonal Antibody Reference Material 8671

Bai

Tian

2022

J. Proteome Res.

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The characteristics of monoclonal antibodies (mAbs) cohering various function effectors show great expectation in therapy. Glycosylation, one of the common post-translational modifications, deeply influences cohesion. It is necessary to grasp monosaccharide composition/sequence and glycan structures in mAbs. There has been comprehensive mass spectrometry characterization of N-glycosylation of mAbs, and monosaccharide compositions are deduced according to known biosynthetic rules. Our recently developed intact N-glycopeptide search engine GPSeeker has made structure-specific characterization of Nglycosylation possible with structure-diagnostic fragment ions from selective fragmentation of N-glycan moieties. Here, we report our structure-specific N-glycoproteomics characterization of NIST monoclonal antibody reference material 8671 using GPSeeker, and 59 N-glycan structures (including 16 pairs of isomers) are characterized.

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Gain-of-glycosylation in breast multi-drug-resistant MCF-7 adenocarcinoma cells and cancer stem cells characterized by site- and structure-specific N-glycoproteomics

Qin

Tian

2023

Analytica Chimica Acta

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Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer

Cited by 14 publications

References 116 publications

Recent Advances in Mass Spectrometry-Based Glycomic and Glycoproteomic Studies of Pancreatic Diseases

Recent Advances in Mass Spectrometry-Based Glycomic and Glycoproteomic Studies of Pancreatic Diseases

Structure-Specific N-Glycoproteomics Characterization of NIST Monoclonal Antibody Reference Material 8671

Gain-of-glycosylation in breast multi-drug-resistant MCF-7 adenocarcinoma cells and cancer stem cells characterized by site- and structure-specific N-glycoproteomics

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