Gain-of-glycosylation in breast multi-drug-resistant MCF-7 adenocarcinoma cells and cancer stem cells characterized by site- and structure-specific N-glycoproteomics

Qin, Suideng; Tian, Zhixin

doi:10.1016/j.aca.2023.341029

Analytica Chimica Acta

2023

DOI: 10.1016/j.aca.2023.341029

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Gain-of-glycosylation in breast multi-drug-resistant MCF-7 adenocarcinoma cells and cancer stem cells characterized by site- and structure-specific N-glycoproteomics

Suideng Qin

Zhixin Tian

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2024

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Cited by 3 publications

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Analysis of mutation‐originated gain‐of‐glycosylation using mass spectrometry‐based N‐glycoproteomics

Yang,

Tian

2024

Rapid Comm Mass Spectrometry

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RationaleA general N‐glycoproteomics analysis pipeline has been established for characterization of mutation‐related gain‐of‐glycosylation (GoG) at intact N‐glycopeptide molecular level, generating comprehensive site and structure information of N‐glycosylation.MethodsThis study focused on mutation‐originated GoG using a mass spectrometry‐based N‐glycoproteomics analysis workflow. In brief, GoG intact N‐glycopeptide databases were built, consisting of 2701 proteins (potential GoG N‐glycosites and amino acids derived from MUTAGEN, VARIANT and VAR_SEQ in UniProt) and 6709 human N‐glycans (≤50 sequence isomers per monosaccharide composition). We employed the site‐ and structure‐specific N‐glycoproteomics workflow utilizing intact N‐glycopeptides search engine GPSeeker to identify GoG intact N‐glycopeptides from parental breast cancer stem cells (MCF‐7 CSCs) and adriamycin‐resistant breast cancer stem cells (MCF‐7/ADR CSCs).ResultsWith the criteria of spectrum‐level false discovery rate control of ≤1%, we identified 87 and 94 GoG intact N‐glycopeptides corresponding to 37 and 35 intact N‐glycoproteins from MCF‐7 CSCs and MCF‐7/ADR CSCs, respectively. Micro‐heterogeneity and macro‐heterogeneity of N‐glycosylation from GoG intact N‐glycoproteins with VAR_SEQ and VARIANT were found in both MCF‐7 CSCs and MCF‐7/ADR CSCs systems.ConclusionsThe integration of site‐ and structure‐specific N‐glycoproteomics approach, conjugating with GoG characterization, provides a universal workflow for revealing comprehensive N‐glycosite and N‐glycan structure information of GoG. The analysis of mutation‐originated GoG can be extended to GoG characterization of other N‐glycoproteome systems including complex clinical tissues and body fluids.

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Analysis of mutation‐originated gain‐of‐glycosylation using mass spectrometry‐based N‐glycoproteomics

Yang,

Tian

2024

Rapid Comm Mass Spectrometry

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Magnetic mesoporous materials and MALDI-TOF MS in peptidomics: From enrichment techniques to disease applications

Xu,

Zhang,

Sun

et al. 2024

TrAC Trends in Analytical Chemistry

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Mass spectrometry-based structure-specific <?A3B2 pi6?> <italic>N-</italic>glycoproteomics and biomedical applications

Bi,

Tian

2024

ABBS

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Gain-of-glycosylation in breast multi-drug-resistant MCF-7 adenocarcinoma cells and cancer stem cells characterized by site- and structure-specific N-glycoproteomics

Cited by 3 publications

References 19 publications

Analysis of mutation‐originated gain‐of‐glycosylation using mass spectrometry‐based N‐glycoproteomics

Analysis of mutation‐originated gain‐of‐glycosylation using mass spectrometry‐based N‐glycoproteomics

Magnetic mesoporous materials and MALDI-TOF MS in peptidomics: From enrichment techniques to disease applications

Mass spectrometry-based structure-specific <?A3B2 pi6?> <italic>N-</italic>glycoproteomics and biomedical applications

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Gain-of-glycosylation in breast multi-drug-resistant MCF-7 adenocarcinoma cells and cancer stem cells characterized by site- and structure-specific N-glycoproteomics

Cited by 3 publications

References 19 publications

Analysis of mutation‐originated gain‐of‐glycosylation using mass spectrometry‐based N‐glycoproteomics

Analysis of mutation‐originated gain‐of‐glycosylation using mass spectrometry‐based N‐glycoproteomics

Magnetic mesoporous materials and MALDI-TOF MS in peptidomics: From enrichment techniques to disease applications

Mass spectrometry-based structure-specific &lt;?A3B2 pi6?&gt; &lt;italic&gt;N-&lt;/italic&gt;glycoproteomics and biomedical applications

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Product

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About

Mass spectrometry-based structure-specific <?A3B2 pi6?> <italic>N-</italic>glycoproteomics and biomedical applications