Beiträge zur Taxonomie der Gattung <i>Calendula</i> II. Taxonomische Revision der südeuropäischen perennierenden <i>Calendula</i>‐Sippen

Ohle, Harald

doi:10.1002/fedr.19740850402

Cited by 19 publications

(8 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nora et al (2013) discuss different hypotheses to explain the chromosome number and the genome size in C. officinalis. Both hypotheses, chromosome losses in C. maroccana (Heyn and Joel, 1983) or hybridization between C. stellata (2n = 14) and a species with 2n = 18 (e.g., C. maroccana, C. eckerleinii) followed by chromosome doubling (Ohle, 1974) are not well supported when the genome sizes of these species are taken into account. Other techniques like genomic in situ hybridization (GISH) using probes of the possible ancestors could be used to explore what species are involved in the genetic background in C. officinalis as well as to examine the loss of specific chromosomes.…”

Section: Discussionmentioning

confidence: 99%

Artificial Chromosome Doubling in Allotetraploid Calendula officinalis

et al. 2020

View full text Add to dashboard Cite

Calendula officinalis L. is known as an ornamental plant as well as a source of biochemical compounds used in cosmetics and industry. C. officinalis has a complex karyotype. Published chromosome numbers differ between 2n = 4x = 28 or 32. We have estimated genome sizes in nine commercial cultivars and evaluated the ploidy level by karyotyping and fluorescent in situ hybridization (FISH) using 5S and 45S rDNA loci. The detection of chromosome sets of two rather than four homologues would suggest that C. officinalis has an allotetraploid background. In addition, four signals for 45S but only two for 5S were found by using FISH. Artificial chromosome doubling is a common technique in plant breeding, as polyploidization results in several consequences for plant growth and development. Especially the suggested allotetraploid background in C. officinalis is interesting when examining the effect of chromosome doubling on the plant phenotype. Here we describe chromosome doubling of three allotetraploid cultivars of C. officinalis, 'Nova,' 'WUR 1553-7' and 'Orange Beauty'. Three antimitotic agents-colchicine, oryzalin and trifluralin-were used in different concentrations to find the combination of the best agent and the best dosage to obtain octaploids. For all three cultivars a few octaploids were obtained. A concentration of 200 and 400 ppm of colchicine was most efficient for chromosome doubling in 'Nova' and 'Orange Beauty,' respectively. For 'WUR 1553-7' the treatment with 20 ppm oryzalin was also effective. Cell numbers and first observations of the phenotype in the chromosome doubled plants show thicker leaves and bigger cells, as commonly observed after ploidy doubling. Due to the low number of chromosome doubled plants obtained more elaborate phenotyping will be performed on following generations cultivated under field conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

Artificial Chromosome Doubling in Allotetraploid Calendula officinalis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…( ¼ C. stellata Cav. ), was demonstrated by Lanza (1919) in ex situ experiments; hybridization in wild populations, although suspected to occur (Ohle 1974;Troia 2011), has never been verified. Here, our two lines of evidence (palynological and molecular), obtained separately but with a common goal, using different plants collected from one common and several disparate populations of the taxa of interest, are brought to bear on the question of whether hybridization between C. maritima and Cs.…”

Section: Introductionmentioning

confidence: 89%

“…Some disagreement exists about the taxonomic status of C. maritima in various treatments of Calendula (e.g., C. incana subsp. maritima in Ohle 1974;C. suffruticosa subsp.…”

Section: Introductionmentioning

confidence: 99%

Hybridization and competition between the endangered sea marigold (Calendula maritimaAsteraceae) and a more common congener

Plume

Raimondo

Troìa

2013

Plant Biosystems - An International Journal Dealing with all As

View full text Add to dashboard Cite

Hybridization and competition between the endangered sea marigold (Calendula maritima, Asteraceae) and a more common congener ARTICLE in PLANT BIOSYSTEMS · FEBRUARY 2015 Impact Factor: 1.91 · AbstractOccurrences of hybridization between the rare, endangered Sicilian endemic, Calendula maritima, and other congeners in the wild have been suspected by several authors, but never demonstrated. In San Cusumano (Trapani), one of the only three Sicilian mainland populations, C. maritima occurs in close proximity to the more common congener C. suffruticosa subsp. fulgida, and individuals morphologically intermediate between the two taxa have been observed. In order to determine whether hybridization is occurring at this site, and, if so, begin to assess the impact it could have on C. maritima, two independent tests of hybridity were conducted by studying (1) the pollen morphology and viability and (2) sequence variation in one chloroplast marker (atpI-atpH) and in the nuclear ribosomal internal transcribed spacer region (ITS). Both palynological and molecular data provided evidence of hybridization between C. maritima and C. suffruticosa subsp. fulgida, and suggest that although fertility of hybrids appeared to be reduced, they are likely capable of back-crossing with parents. Thus, hybridization may be putting C. maritima at risk of extinction via introgression. Anthropogenic habitat disturbance and expansion of C. suffruticosa subsp. fulgida into remaining populations of C. maritima could pose a serious threat to its successful conservation.

show abstract

“…The compounds that showed higher positive correlations with PC 2 were, in a descending order of importance, αamyrin (30), untriacontane (42), lanosterol (29), 13-hexyloxacyclotridec-10-en-2one (3), phytol (23), phytyl palmitate (8), campesterol (33) and triacontane (41), and higher negative loadings, in an ascending order of importance, for nine compounds β-amyrin (28), neophytadiene (12), 8,14cedranoxide (17), 1H-3a,7-methanoazulen-6-ol (15), 1ethyl-4,4-dimethylcyclohex-2-en-1-ol (9), 1(2H)-naphthalenone (10), myristic acid (1), 1,3-benzenediol (38) and γ-eudesmol (21)) (Table S4). PC 3 presented seven compounds reaching positive loadings greater than 0.6, which were di-epi-1,10-cubenol (19), germanicol (25), humulane-1,6-dien-3-ol (18), THMA (13), lignoceric acid (7), α-bisabolol (11) and 1-tetracosanol (36), and six compounds showed a negative loading below À 0.6, which were HETU (24), (+)-β-guaiene (14), HMTU (26), selina-6-en-4-ol (16), MDN (20) and 2,6,6,9,2',6',6',9'-Octamethyl- [8,8']bi-[tricyclo[5.4.0.0 (2,9) ]undecyl] (OBTU, 27) (Table S4).…”

Section: Principal Component Analysismentioning

confidence: 99%

Chemical Variability of Hexane Extracts from Five Subspecies of Calendula suffruticosa from Spain

Ohse

Marques

Silveira

et al. 2022

Chemistry & Biodiversity

View full text Add to dashboard Cite

The chemical profile of the hexane extracts of the subspecies carbonellii, greuteri, marginata, trialata, and vejerensis of Calendula suffruticosa growing in Spain, herein described for the first time, were studied to access their value as a chemo taxonomical tool and search for potentially useful compounds. The subsp. greuteri and carbonellii showed higher extract yields. Terpenoids were the most abundant chemical class in subsp. carbonellii, greuteri, trialata, and vejerensis, while alkanes were the most abundant in subsp. marginata. Differences in chemical constituents were identified among the subspecies of C. suffruticosa analysed, which the PCA can prove. The subsp. trialata and greuteri showed more significant phytochemical similarity, which might indicate genetic proximity between these two subspecies. C. suffruticosa subsp. marginata presented the fewest number of compounds and in the smallest quantities, and C. suffruticosa subsp. vejerensis presented the largest number, however, both showed no alcohols. Furthermore, some of the compounds found in significant amounts are known for their pharmacological and nutraceutical properties, denoting potential use.

show abstract

Beiträge zur Taxonomie der Gattung Calendula II. Taxonomische Revision der südeuropäischen perennierenden Calendula‐Sippen

Cited by 19 publications

References 4 publications

Artificial Chromosome Doubling in Allotetraploid Calendula officinalis

Artificial Chromosome Doubling in Allotetraploid Calendula officinalis

Hybridization and competition between the endangered sea marigold (Calendula maritimaAsteraceae) and a more common congener

Chemical Variability of Hexane Extracts from Five Subspecies of Calendula suffruticosa from Spain

Contact Info

Product

Resources

About