Beitrag zur Gefrierkonservierung pathogener Amoeben und Trichomonaden

Abstract

A viability assay for Tetrahymena pyriformis. 1. Gen. Microbiol. 68, 249-51. 5. Hogg JD, Kornberg HL. 1963. The metabolism of Czcompounds in microorganisms. A. Role of the glyoxalate cycle in protozoal glyconeogenesis. Biochem. J. 86, 462-8. 6. Hwang S-W, Davis EE, Alexander MT. 1964. Freezing and viability of Tetrahymena pyriformis in dimethylsulphoxide. Science 7. Klammer B, Fennel1 RA. 1963. Acid phosphatase activity during growth and synchronous division of Tetrahymena pyriformis W. Exp. Cell Res. 29, 166-75. 8. Lazarus LH, Scherbaum OH. 1967. Isolation and specificity of the intracellular ribonuclease from Tetrahymena pyriformis. Biochim. Biophys. Acta 142, 368-84. 9. -,-1967. Activity of ribonuclease, acid phosphatase and phosphodiesterase in Tetrahymena pyriformis during growth. J . Cell Biol. 36, 415-8. 10. Lee D. 1969. Photolysis in a culture medium for Tetrahymena pyriformis. ]. Cell. Physiol. 74, 295-8. 11. -1970. Variation in the latency of acid phosphatase and ribonuclease throughout the growth cycle of Tetrahymena pyriformis. J . Cell. Physiol. 76, 17-22. 12. Lloyd D, Brightwell R, Venables SE, Roach GI, Turner G. 197 1. Subcellular fractionation of Tetrahymena pyriformis S T by zonal centrifugation: changes in activities and distribution of 1&, 64-5. enzymes during the growth cycle and on starvation. J . Gen. Microbiol. 65, 209-23. 13. Lovelock JE. 1953. T h e haemolysis of human red blood cells by freezing and thawing. Biochim. Biophys. Acta 10, 414-26. 14. -1953. The mechanism of the protective action of glycerol against haemolysis by freezing and thawing. Biochim. Biophys. Acta 11, 28-36. 15. -1954. The protective action of neutral solutes against haemolysis by freezing and thawing. Biochem. 1. 56, 265-70. 16. Mazur P. 1970. Cryobiology: the freezing of biological systems. Science 168, 939-49. 17. Pelczar MJ. 1962. Low temperature microbiology-bacterial survival and interactions. Proc. Low Temperature Microbiology Symp., Campbell Soup Company, Camden, N.J, pp. 133-7. 18. Simon EM. 1972. Freezing and storage in liquid nitrogen of axenically and monoxenically cultured Tetrahymena pyriformis. Cryobiology 9, 75-81. 19. -, Hwang S-W 1967. Tetrahymena: effect of freezing and subsequent thawing on breeding performance. Science 155, 20. -, Schneller MV. 1973. The preservation of ciliated protozoa at low temperature. Cryobiology 10, 421-6. 21. Smith AU. 1961. Biological Effects of Freezing and Supercooling. Arnold, London, pp. 406-46. 22. Sutherland A, Antipa GA, Ehret CF. 1973. Ultracytochemical observations of glycogen synthesis and storage during infradian growth of Tetrahymena pyriformis. 1. Cell Biol. 58, 240-4. 23. Wang G-T, Marquardt WC. 1966. Survival of Tetrahymena pyriformis and Paramecium aurelia following freezing. J . Protorool. 694-6. 13, 123-8.SYNOPSIS. Unstimulated mouse peritoneal exudate cells were cultured on coverslips in Medium 199 containing 10% (v/v) calf serum. Cytochalasin B dissolved in dimethyl sulphoxide (DMSO) and diluted in Medium 199 was added to cultures to give final conc...