Isolierung von Amoeben des Typs ?Hartmannella-Acanthamoeba? und ?Naegleria? aus Kaltbl�tern

Frank, Werner; Bosch, I.

doi:10.1007/bf00329149

Cited by 17 publications

(8 citation statements)

References 15 publications

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“…Nevertheless, several studies have shown that freeliving amoebae can cause disease in cold-blooded animals, including all major groups of the Reptilia (Bosch and Deichsel 1972;Frank and Bosch 1972). To our knowledge, this is the ®rst description of an isolation of free-living amoebae from a basilisc.…”

Section: Resultsmentioning

confidence: 91%

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Isolation and identification by partial sequencing of the 18S ribosomal gene of free-living amoebae from necrotic tissue of Basiliscus plumifrons (Sauria: Iguanidae)

Walochnik

Hassl

Simon

et al. 1999

Parasitology Research

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A 3-year-old Basiliscus plumifrons developed a necrotic lesion on the tail resulting from nodules of unknown etiology. Investigation of necrotic tissue revealed several gram-negative bacteria as well as three different species of free-living amoebae. The amoebae were identified by morphological characters as belonging to the genera Acanthamoeba, Echinamoeba, and Naegleria, respectively. Partial sequencing of the 18S ribosomal gene was performed for reliable systematic determination. Two of the isolates showed thermotolerance. No isolate was growable in conventional liquid media, but the Acanthamoeba strain readily grew on a human cell line (HEp2). It remains unclear whether the amoebae fed on the coexisting bacteria or on host tissue.

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Section: Resultsmentioning

confidence: 91%

“…Apart from their occurrence in fresh water, seawater, and soil, they have been found in several animals as well as in Amphibia (Frank and Bosch 1972;Madrigal Sesma et al 1988) and Reptilia (Bosch and Deichsel 1972;Frank and Bosch 1972;Madrigal Sesma et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification by partial sequencing of the 18S ribosomal gene of free-living amoebae from necrotic tissue of Basiliscus plumifrons (Sauria: Iguanidae)

Walochnik

Hassl

Simon

et al. 1999

Parasitology Research

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“…Further, S. diploidea , an amoeba that has been identified in fecal specimens of cattle and elk has also been implicated as an agent of meningoencephalitis in a human (Gelman et al 2001). Members belonging to Hartmannella, Acanthamoeba, Vahlkampfia, Naegleria , and Entamoeba have also been isolated from the brain and intestines of reptiles (Bosch and Deichsel 1972; Frank and Bosch 1972; Sesma and Ramos 1989). Walochnik et al (1999) isolated several free‐living amoebae from a necrotic lesion on the tail of a basilisc lizard and identified them as A. castellanii, N. gruberi , and Echinamoeba sp.…”

Section: Discussionmentioning

confidence: 99%

Paravahlkampfia francinae n. sp. Masquerading as an Agent of Primary Amoebic Meningoencephalitis

Visvesvara

Sriram

Qvarnström

et al. 2009

J Eukaryotic Microbiology

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Paravahlkampfia francinae n. sp., a new species of the free-living amoeba genus Paravahlkampfia, designated as CDC:V595, was isolated from the cerebrospinal fluid of a patient with headache, sore throat, and vomiting, typical symptoms of primary amoebic meningoencephalitis (PAM) caused by Naegleria fowleri. The isolate grew at 33 degrees C, 37 degrees C, 40 degrees C, and 42 degrees C and destroyed mammalian cell cultures. However, it did not kill young mice upon intranasal inoculation. P. francinae does not produce flagellates and does not grow on agar plates coated with Gram-negative bacteria such as Escherichia coli, the usual food source of Paravahlkampfia ustiana, the type species of the genus. The trophozoite at light microscopy exhibited eruptive locomotion and possessed a single vesicular nucleus. Ultrastructurally, the trophozoites had numerous mitochondria with discoidal cristae but did not have a Golgi apparatus. The trophozoites differentiated into cysts after consuming most of the monolayer. The cyst had an inner well-differentiated endocyst and an outer thin, wrinkled, and wavy ectocyst with no pores. During excystation trophozoites ruptured the cyst wall and emerged from the cysts. A unique feature seen in the cysts was the presence of bacterial endosymbionts, both in the endoplasm and within the cyst wall. Full-length sequencing analysis of the 18S and 5.8S RNA genes of P. francinae showed that they were distinct from those of other Paravahlkampfia species. The patient recovered within a few days indicating that some of the previously reported cases of PAM that survived may have been due to P. francinae.

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“…They include the recovery of Naegleria from the gills of fish (122), from snails (81,103), and from various reptiles (57). Acanthamoeba, a related freshwater ameba, has been detected in visceral lesions of a dog (4), in pulmonary lesions of a buffalo {53), in a bull (96), in renal granulomas in a gold fi sh (131), and cultured from clinical specimens and tissues of a variety of diseased domes tic animals (77).…”

Section: Animal Infectionmentioning

confidence: 99%

Primary Amebic Meningoencephalitis and the Biology of Naegleria Fowleri

John¹

1982

Annu. Rev. Microbiol.

161

114

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Isolierung von Amoeben des Typs ?Hartmannella-Acanthamoeba? und ?Naegleria? aus Kaltbl�tern

Cited by 17 publications

References 15 publications

Isolation and identification by partial sequencing of the 18S ribosomal gene of free-living amoebae from necrotic tissue of Basiliscus plumifrons (Sauria: Iguanidae)

Isolation and identification by partial sequencing of the 18S ribosomal gene of free-living amoebae from necrotic tissue of Basiliscus plumifrons (Sauria: Iguanidae)

Paravahlkampfia francinae n. sp. Masquerading as an Agent of Primary Amoebic Meningoencephalitis

Primary Amebic Meningoencephalitis and the Biology of Naegleria Fowleri

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