Behavioural screening of zebrafish using neuroactive traditional Chinese medicine prescriptions and biological targets

Wang, Yanan; Hou, Yuanyuan; Sun, Mingzhu; Zhang, Chunyang; Bai, Gang; Zhao, Xin; Feng, Xizeng

doi:10.1038/srep05311

Cited by 20 publications

(19 citation statements)

References 56 publications

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“…Traditional Chinese Medicine (TCM), as a complementary and alternative medicine, has been proven to possess satisfactory effects against depression and its clinical complications. When treating depression, TCM starts from the whole body, considering not only the psychological problems that result from a patient's nervous system disorder but also the changes of the zang-fu organs, qi, and blood (Wang et al, 2014). Si-Ni-San (SNS), a famous formula, was first recorded in the "Treatise on Febrile Diseases" by Zhong-jing Zhang, a famous doctor in the Han Dynasty.…”

Section: Highlights Introductionmentioning

confidence: 99%

Si-Ni-San Prevents Reserpine-Induced Depression by Inhibiting Inflammation and Regulating CYP450 Enzymatic Activity

et al. 2020

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Depression is becoming a major public health concern worldwide. Si-Ni-San (SNS) is a famous formula in Traditional Chinese Medicine (TCM) with potent antidepressant effects. However, the antidepressant mechanism of SNS has not been clearly elucidated. This study was performed to verify whether the antidepressant effects of SNS were related to its anti-inflammatory effects, the levels of brain-derived neurotrophic factor (BDNF) and Cytochrome P450 (CYP450) enzymatic activity. In our study, behavioral tests such as the forced swim test, sucrose preference test and open-field test were evaluated to ensure the establishment of depressive rats. The levels of IL-1b, IL-6, and TNF-a in the serum, liver, and hippocampus of rats were measured by enzyme-linked immunosorbent assays (ELISA). Furthermore, the key proteins NF-kB, BDNF, and TrkB were analyzed by Western blot (WB) analysis in the hippocampus. In addition, CYP450 enzymatic activity analysis was performed using LC-MS/MS in conjunction with drug and statistics (DAS 3.0) after oral administration of six probe drugs. Our results showed that SNS attenuated reserpineinduced increases in IL-1b, IL-6, and TNF-a expression in the serum, liver, and hippocampus. The levels of NF-kB, BDNF, and TrkB in the hippocampus of depressive rats were also altered. According to the pharmacokinetic parameters, SNS had moderate inhibitory effects in the reserpine-induced depression model on CYP1A2, CYP2D1, CYP2E1, and CYP3A2, but no significant metabolic changes to CYP2C6 and CYP2D2. These findings suggested that SNS has a protective effect on reserpine-induced depressive rats, which may be related to the improvement of the inflammatory factors, the level of BDNF and the activity of CYP450 enzymes.

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Section: Highlights Introductionmentioning

confidence: 99%

Si-Ni-San Prevents Reserpine-Induced Depression by Inhibiting Inflammation and Regulating CYP450 Enzymatic Activity

et al. 2020

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“…Each compound was assigned a behavioural fingerprint and in each, different colours indicated the value of different behavioural parameters. By extracting the colour value (red, green and blue), the corresponding behavioural parameters were calculated (24). Among >500 compounds with significantly altered behaviour compared with controls, ~100 well-characterized compounds were selected for correlation analysis in the present study.…”

Section: Reverse Transcription-quantitative Pcr (Rt-qpcr)mentioning

confidence: 99%

Effects of tenuifolin on rest/wake behaviour in zebrafish

Chen

Peng²,

Pei

et al. 2020

Exp Ther Med

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Insomnia is a common sleep disorder with a high prevalence and substantial adverse consequences. There is growing interest in identifying novel therapeutics from herbal medicine. Tenuifolin is a major constituent of the well-known anti-insomnia herb Radix Polygala. The present study investigated the neural activity in response to tenuifolin during rest/wake behaviour in zebrafish and identified the potential biological signalling pathways involved. An automatic video tracking system was used to monitor the behavioural response of zebrafish larvae for 24 h after treatment with tenuifolin. In total, six rest/wake parameters were measured and visualized with a behavioural fingerprint. Time series analysis was conducted by averaging the total rest and waking activity in 10 min intervals. A correlation analysis was performed between tenuifolin and well-known compounds to analyse the underlying biological signalling pathways. Reverse transcription-quantitative PCR was also performed to detect the effects of tenuifolin on the transcription of interesting genes associated with the signalling pathways that were potentially involved. The present results suggested tenuifolin significantly increased the total rest time during the dark phase, with a slight effect on the waking activity in zebrafish larvae. This behavioural phenotype induced by tenuifolin is similar to that of selective serotonin reuptake inhibitors and gamma-aminobutyric acid (GABA) agonists. Furthermore, the expression levels of GABA transporter 1 were significantly increased after tenuifolin treatment. No significant difference was determined in other associated genes in untreated control and tenuifolin-treated larvae. The present results suggested that tenuifolin caused sleep-promoting activity in zebrafish and that these effects may be mediated by the serotoninergic systems and the GABAergic systems.

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“…Recently, Wang et al (2014) monitored the behavior of zebrafish larvae after exposure to radix ginseng by using an automatic video-tracking system. They found that the curative effects of the prescriptions were dosedependent.…”

Section: Chinese Ginseng Ashwagandha and Cannabinoidsmentioning

confidence: 99%