Behaviour of 14C-sulfadiazine and 14C-difloxacin during manure storage

Lamshöft, Marc; Sukul, Premasis; Zühlke, Sebastian; Spiteller, Michael

doi:10.1016/j.scitotenv.2009.12.010

Cited by 59 publications

(26 citation statements)

References 30 publications

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“…If these compounds are not degraded during manure storage, they will enter the soil via manure fertilization (Du and Liu, 2011). This is the case of sulfadiazine (SDZ), a bacteriostatic antibiotic of the sulfonamide class frequently used in pig husbandry (Lamshöft et al, 2010). Once in the soil, antibiotics might alter microbial community structure and function (Ding and He, 2010).…”

Section: Introductionmentioning

confidence: 99%

Drying and rewetting events change the response pattern of nitrifiers but not of denitrifiers to the application of manure containing antibiotic in soil

Radl

Kindler

Welzl

et al. 2015

Applied Soil Ecology

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A B S T R A C TApplication of manure for soil fertilization is a common practice in arable and pasture soils. As antibiotics are regularly used in animal husbandry, these compounds often enter the soil via manure application. The effects of antibiotics on microbial communities, however, might depend on soil moisture, as water availability may influence both the fate of the compound and the activity of the microbes. To test this hypothesis, we investigated the effects of the application of manure containing the antibiotic sulfadiazine (SDZ) on the abundance and activity of nitrifies and denitrifies in soil, based on the copy number of marker genes and their related potential activities, as affected by different moisture regimes. We observed significant effects of SDZ on potential denitrification activity, but those were not influenced by the soil moisture regime. Nevertheless, neither SDZ nor changes on moisture significantly affected the abundance of denitrifies. In contrast, both potential nitrification activity and abundance of ammonia oxidizing bacteria were significantly affected by the application of manure containing SDZ and moisture regime. Interestingly, no effects were observed for ammonia oxidizing archaea. Overall, our data show that soil moisture modulates the effects of antibiotics in soil microbial communities, and we recommend to include this parameter in the risk assessment of new chemicals.

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Section: Introductionmentioning

confidence: 99%

Drying and rewetting events change the response pattern of nitrifiers but not of denitrifiers to the application of manure containing antibiotic in soil

Radl

Kindler

Welzl

et al. 2015

Applied Soil Ecology

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“…More than 96% of the SDZ administered to pigs can be recovered in manure as the parent antibiotic compound or as its metabolites (3). Through field fertilization by manuring, SDZ and its metabolites enter arable soils in bioactive amounts (4,5). Although the concentration of readily extractable SDZ in soil was reported to decrease rapidly (6,7), SDZ might still select resistant populations and stimulate horizontal spread of antibiotic resistance genes, leading to an increased abundance of bacterial populations carrying resistance genes (reviewed in reference 8).…”

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confidence: 99%

Increased Abundance and Transferability of Resistance Genes after Field Application of Manure from Sulfadiazine-Treated Pigs

Jechalke¹,

Kopmann

Rosendahl

et al. 2013

Appl Environ Microbiol

146

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c Spreading manure containing antibiotics in agriculture is assumed to stimulate the dissemination of antibiotic resistance in soil bacterial populations. Plant roots influencing the soil environment and its microflora by exudation of growth substrates might considerably increase this effect. In this study, the effects of manure from pigs treated with sulfadiazine (SDZ), here called SDZ manure, on the abundance and transferability of sulfonamide resistance genes sul1 and sul2 in the rhizosphere of maize and grass were compared to the effects in bulk soil in a field experiment. In plots that repeatedly received SDZ manure, a significantly higher abundance of both sul genes was detected compared to that in plots where manure from untreated pigs was applied. Significantly lower abundances of sul genes relative to bacterial ribosomal genes were encountered in the rhizosphere than in bulk soil. However, in contrast to results for bulk soil, the sul gene abundance in the SDZ manure-treated rhizosphere constantly deviated from control treatments over a period of 6 weeks after manuring, suggesting ongoing antibiotic selection over this period. Transferability of sulfonamide resistance was analyzed by capturing resistance plasmids from soil communities into Escherichia coli. Increased rates of plasmid capture were observed in samples from SDZ manure-treated bulk soil and the rhizosphere of maize and grass. More than 97% of the captured plasmids belonged to the LowGC type (having low G؉C content), giving further evidence for their important contribution to the environmental spread of antibiotic resistance. In conclusion, differences between bulk soil and rhizosphere need to be considered when assessing the risks associated with the spreading of antibiotic resistance.

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“…When 14 C-labeled SDZ was administered to pigs, only 4% of the radioactivity remained in the pigs after 10 days and 44% was still detectable in the collected manure as the parent substance, besides 4-hydroxysulfadiazine (4-OH-SDZ) and N-acetyl-sulfadiazine (Ac-SDZ) (2). Even during a longer storage of pig manure, SDZ and its principal biotransformation products were shown to be rather stable (3).…”

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Degradation of Sulfadiazine by Microbacterium lacus Strain SDZm4, Isolated from Lysimeters Previously Manured with Slurry from Sulfadiazine-Medicated Pigs

Tappe

Herbst

Hofmann

et al. 2013

Appl Environ Microbiol

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b Sulfadiazine (SDZ)-degrading bacterial cultures were enriched from the topsoil layer of lysimeters that were formerly treated with manure from pigs medicated with 14 C-labeled SDZ. The loss of about 35% of the applied radioactivity after an incubation period of 3 years was attributed to CO 2 release due to mineralization processes in the lysimeters. Microcosm experiments with moist soil and soil slurries originating from these lysimeters confirmed the presumed mineralization potential, and an SDZ-degrading bacterium was isolated. It was identified as Microbacterium lacus, denoted strain SDZm4. During degradation studies with M. lacus strain SDZm4 using pyrimidine-ring labeled SDZ, SDZ disappeared completely but no 14 CO 2 was released during 10 days of incubation. The entire applied radioactivity (AR) remained in solution and could be assigned to 2-aminopyrimidine. In contrast, for parallel incubations but with phenyl ring-labeled SDZ, 56% of the AR was released as 14 CO 2 , 16% was linked to biomass, and 21% remained as dissolved, not yet identified 14 C. Thus, it was shown that M. lacus extensively mineralized and partly assimilated the phenyl moiety of the SDZ molecule while forming equimolar amounts of 2-aminopyrimidine. This partial degradation might be an important step in the complete mineralization of SDZ by soil microorganisms.

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Behaviour of 14C-sulfadiazine and 14C-difloxacin during manure storage

Cited by 59 publications

References 30 publications

Drying and rewetting events change the response pattern of nitrifiers but not of denitrifiers to the application of manure containing antibiotic in soil

Drying and rewetting events change the response pattern of nitrifiers but not of denitrifiers to the application of manure containing antibiotic in soil

Increased Abundance and Transferability of Resistance Genes after Field Application of Manure from Sulfadiazine-Treated Pigs

Degradation of Sulfadiazine by Microbacterium lacus Strain SDZm4, Isolated from Lysimeters Previously Manured with Slurry from Sulfadiazine-Medicated Pigs

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