Behaviors of Microtubules (MTs) Driven by Biological Motors (Dynein c) at Collisions Against Micro-Fabricated Tracks and MTs for Potential Nano-Bio-Machines

Abstract

In vitro motility assays, where protein motors (attached to a surface) move protein filaments, have been used for investigating protein motors' functions. In recent decades, these assays are extended to explore potential applications of motor proteins as biological motors in nano-bio-machine development. Recent attempts include fabricating micrometer-scale tracks on the surface to confine and guide the flow of bio-filaments as a power transfer medium driven by the motor proteins. Understanding the interaction between bio-filaments and fabricated tracks as well as the mutual interaction between bio-filaments is of importance to the design of potential nano-bio-machines. In this study, we investigate the behaviors of a microtubule driven by axonemal dynein at the collision against another microtubule and micro-fabricated walls, respectively. Based on experimental observations, we propose a model to study possible mechanisms for the microtubule-microtubule and microtubule-wall interactions, which involve bumping force, bending moment and torque generation.

Understanding the Guiding of Kinesin/Microtubule-Based Microtransporters in Microfabricated Tracks

“…For example, Chen et al used a 2D simulation to study interactions between MTs propelled by dynein motors and between MTs and guiding walls. 47 Nedelec and colleagues developed a simulation package 48 and used this to design guiding tracks, which were created by patterns of kinesin and hypothetical minus-end-directed motors on planar surfaces with various functions, such as directed transport and polarity sorting. 49 In addition, they also studied guiding events performed by chemical edges with their 2D simulation.…”

“…Currently, there are simulations that explicitly model cytoskeletons and their associated motor proteins, and some of these simulations have been used to investigate the guiding. For example, Chen et al used a 2D simulation to study interactions between MTs propelled by dynein motors and between MTs and guiding walls . Nedelec and colleagues developed a simulation package and used this to design guiding tracks, which were created by patterns of kinesin and hypothetical minus-end-directed motors on planar surfaces with various functions, such as directed transport and polarity sorting .…”

Dispersion and Disparity: Bibliometric and Visualized Analysis of Research on Climate Change Science Communication

“…Based on a path-finding network algorithm (pathfinder), as well as co-citation analysis theory (co-citation), CiteSpace is capable of identifying the research progress, new trends and new developments in a certain field in scientific development and presenting them in a visual form for users’ regular discovery and decision support [ 17 ]. It applies to multivariate, time-phased, dynamic and complex networks and is currently used in more than 10 countries and regions, and is also a commonly used tool in the field of scientometrics [ 18 , 19 , 20 , 21 ].…”

Climbing Rates of Microtubules Propelled by Dynein after Collision with Microfabricated Walls

“…30) The behaviors of microtubules driven by dynein c (axonemal dynein) at the collision with the wall were also examined and simulated. 31) To estimate the efficiency of the height, material, and profile of a wall, we fabricated a guiding wall that makes microtubules collide with a target wall perpendicularly and counted the number of those crossing over it.…”