Understanding the Guiding of Kinesin/Microtubule-Based Microtransporters in Microfabricated Tracks

Ishigure, Yuki; Nitta, T.

doi:10.1021/la5021884

Cited by 17 publications

(11 citation statements)

References 64 publications

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“…Chemical Guiding by the Kinesin Trail: The Case for Pheromonic Interactions. Prior work on filament guiding on motor protein-coated surfaces has shown that filaments follow permanent trails of motors created by chemical surface patterning if the required change in the angle of motion is small (<15°for microtubules), 75,77,78 or if the microtubule is already aligned with a narrow track and the radius of curvature is large enough (>17 μm). 79 Trails of motors have also been printed by deposition of motor-covered microtubules.…”

Section: Resultsmentioning

confidence: 99%

Kinesin-Recruiting Microtubules Exhibit Collective Gliding Motion while Forming Motor Trails

et al. 2020

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Microtubules gliding on surfaces coated with kinesin motors are minimalist experimental systems for studying collective behavior. Collective behavior in these systems arises from interactions between filaments, for example, from steric interactions, depletion forces, or cross-links. To maximize the utilization of system components and the production of work, it is desirable to achieve mutualistic interactions leading to the congregations of both types of agents, that is, cytoskeletal filaments and molecular motors. To this end, we used a microtubule-kinesin system, where motors reversibly bind to the surface via an interaction between a hexahistidine (His 6 ) tag on the motor and a Ni(II)nitrilotriacetic acid (Ni-NTA) moiety on the surface. The surface density of binding sites for kinesin motors was increased relative to our earlier work, driving the motors from the solution to the surface. Characterization of the motor-surface interactions in the absence of microtubules yielded kinetic parameters consistent with previous data and revealed the capacity of the surface to support two-dimensional motor diffusion. The motor density gradually fell over 2 h, presumably due to the stripping of Ni(II) from the NTA moieties on the surface. Microtubules gliding on these reversibly bound motors were unable to cross each other and at high enough densities began to align and form long, dense bundles. The kinesin motors accumulated in trails surrounding the microtubule bundles and participated in microtubule transport.

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Section: Resultsmentioning

confidence: 99%

Kinesin-Recruiting Microtubules Exhibit Collective Gliding Motion while Forming Motor Trails

et al. 2020

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“…To test the hypothesis that the active probes are oriented to the direction which minimized their accumulated bending energy, we performed a simulation study. We adopted and modified a computer simulation of an in vitro gliding assay of microtubules for this study (see ‘Methods' and Supplementary Figs 11 and 12 ) 28 . We simulated the alignment of the active probes induced by the substrate compression.…”

Section: Resultsmentioning

confidence: 99%

“…The simulation method was adopted from a previous work 28 , and modified for this study. Briefly, we simulated the three-dimensional movement of microtubules propelled by kinesin motors on compression of the substrate.…”

Section: Methodsmentioning

confidence: 99%

Sensing surface mechanical deformation using active probes driven by motor proteins

et al. 2016

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Studying mechanical deformation at the surface of soft materials has been challenging due to the difficulty in separating surface deformation from the bulk elasticity of the materials. Here, we introduce a new approach for studying the surface mechanical deformation of a soft material by utilizing a large number of self-propelled microprobes driven by motor proteins on the surface of the material. Information about the surface mechanical deformation of the soft material is obtained through changes in mobility of the microprobes wandering across the surface of the soft material. The active microprobes respond to mechanical deformation of the surface and readily change their velocity and direction depending on the extent and mode of surface deformation. This highly parallel and reliable method of sensing mechanical deformation at the surface of soft materials is expected to find applications that explore surface mechanics of soft materials and consequently would greatly benefit the surface science.

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“…The simulation method was based on our previous work [7], and extended to include applied force to microtubules. In the following, we briefly summarize the simulation method.…”

Section: Simulation Methodsmentioning

confidence: 99%

“…In order to simulate the sidewise slipping and detachments, microtubules and kinesins have to be explicitly modeled. We have recently developed a simulation which explicitly models cytoskeletal filaments and motor proteins [7] [8]. In this study, we extended the simulation to include applied forces, and investigated whether the extended simulation reproduced experimental results.…”

Section: Introductionmentioning

confidence: 99%

Gliding Movements of Microtubule Driven by Kinesin Motors under External Force: Steering and Detachment

Nitta¹,

Kawauchi²

2016

Proceedings of the 9th EAI International Conference on Bio-Inspired Information and Communications Technologies (Formerly BIONE

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Kinesin and microtubule have been utilized for applications, such as molecular communication. In this paper, we describe a computer simulation which reproduced movements of microtubules gliding over kinesin-coated glass surfaces under external force. The simulation qualitatively reproduced experimental results of microtubule movements under external force. This simulation would enable understanding of details of the gliding movements of microtubules under external force.

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Understanding the Guiding of Kinesin/Microtubule-Based Microtransporters in Microfabricated Tracks

Cited by 17 publications

References 64 publications

Kinesin-Recruiting Microtubules Exhibit Collective Gliding Motion while Forming Motor Trails

Kinesin-Recruiting Microtubules Exhibit Collective Gliding Motion while Forming Motor Trails

Sensing surface mechanical deformation using active probes driven by motor proteins

Gliding Movements of Microtubule Driven by Kinesin Motors under External Force: Steering and Detachment

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