Behavioral Responses of thePine Wood Nematodeto Terpenes

Tominaga, Yasuhira; Yamamoto, Masayuki; Kuwahara, Yasumasa; Sugawara, Ryozo

doi:10.1080/00021369.1984.10866171

Cited by 3 publications

(4 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For a long time, 18 compounds of pine trees were known to be attractive to pine wilt nematodes: terpenes (camphene (C 10 H 16 ), 3-carene (C 10 H 16 ), limonene, β-myrcene (C 10 H 16 ), α-pinene, β-pinene (C 10 H 16 ), β-phellandrene (C 10 H 16 )), alcohols (phytol (C 20 H 40 O), geraniol (C 10 H 18 O), nerol (C 10 H 18 O), oleyl alcohol (C 18 H 36 O), ethylene glycol monooleate (C 20 H 38 O 3 )), acids (linoleic (fatty acid, C 18 H 32 O 2 ), palmitoleic (C 16 H 30 O 2 ), oleic (C 18 H 34 O 2 ), and abietic acids (C 20 H 30 O 2 )), monoacylglycerol 1-monooleine (C 21 H 40 O 4 ), and aromatic hydrocarbon toluene (C 7 H 8 ) (Table ). − ,, Later on, three more terpenes were identified as attractants for B. xylophilus : longifolene (C 15 H 24 ), terpinolene (C 10 H 16 ), and camphor (C 10 H 16 O) . The attractivity of CO 2 was demonstrated as well …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Toward Chemical Ecology of Plant-Parasitic Nematodes: Kairomones, Pheromones, and Other Behaviorally Active Chemical Compounds

Čepulytė

Būda

2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

An overview of natural chemical compounds involved in plant-parasitic nematode (PPN) behavior is presented and classified following a system accepted by chemoecologists. Kairomonal and other egg-hatching stimulants, as well as attractants for juveniles, are presented. Sex, aggregation, egg-hatching, and putative diapause PPN pheromones are analyzed and grouped into clusters of primers and releasers. The role of over 500 chemical compounds, both organic and inorganic, involved in PPN behavior is reviewed, with the most widely analyzed and least studied fields of PPN chemical ecology indicated. Knowledge on PPN chemical ecology facilitates environmentally friendly integrated pest management. This could be achieved by disrupting biointeractions between nematodes and their host plants and/or between nematodes. Data on biologically active chemicals reveals targets for resistant plant selection, including through application of gene silencing techniques.

show abstract

Section: Introductionmentioning

confidence: 99%

“…3). [92][93][94][95]97,101 Later on, three more terpenes were identified as attractants for B. xylophilus: longifolene (C 15 H 24 ), 102 terpinolene (C 10 H 16 ), 103 and camphor (C 10 H 16 O). 103 The attractivity of CO 2 was demonstrated as well.…”

Section: ■ Introductionmentioning

confidence: 99%

Toward Chemical Ecology of Plant-Parasitic Nematodes: Kairomones, Pheromones, and Other Behaviorally Active Chemical Compounds

Čepulytė

Būda

2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…22.943852 doi: bioRxiv preprint Pine wilt disease (PWD) constitutes one of the most serious conifer diseases worldwide, affecting Pinus spp. from the Far East forestlands (Japan, China, Korea) [1,2], and from Europe (Portugal and Spain) [3][4][5][6]. PWD is caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle, with PWN transmission being dependent on a vector insect, such as Monochamus alternatus Hope, the main vector in East Asia [7,8].…”

Section: Introductionmentioning

confidence: 99%

Study of the departure of pine wood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) from Monochamus alternatus (Coleoptera: Cerambycidae)

Wang

Chen

Wang

et al. 2020

Journal of Asia-Pacific Entomology

View full text Add to dashboard Cite

In order to study the causes of pine wood nematodes (PWN), Bursaphelenchus xylophilus departure from its vector beetle, Monochamus alternatus, we collected PWNs which were extracted from the newly emerged M. alternatus and the 7d after emergence beetles. The total RNAs of the two groups of PWNs were extracted and transcriptomes sequencing were performed, and the genes expression differences between the two groups of PWN were analyzed. It was found that expression of choline-phosphate cytidylyltransferase gene (pcyt-1) markedly up-regulated. After inhibition of pcyt-1expression by RNA interference, the rate of lipid degradation in PWN decreased significantly, and the motility of PWN also decreased significantly.Analysis identified that phosphatidylcholine can promote the emulsification and degradation of lipid droplets in PWN, which provide energy for PWN departure from M. alternatus. The up-regulation of gene pcyt-1 is an important internal trigger for PWN departure from the beetles.Flurometer (Life Technologies, CA, USA). RNA integrity was assessed using the videos being taken under a microscope (Zeiss Axio lmager.M2, Carl Zeiss, Gottingen, Germany). After h of RNAi (at which time, the lipids contents of the two PWN groups were markedly different.), the lipids of PWN were stained with oil red O [33].PWN was washed into a 1.5ml eppendorf tube with M9 buffer (standing for 10min at 2 4°C, discarding the supernatant and repeating it once). The lipids of PWN were fixed 2 with 4% paraformaldehyde at 4°C for 30 minutes, then frozen in a -80°C refrigerator 2 for 15 minutes, then thawed rapidly in 43°C water bath. The sample was centrifuged 2 for 1 min with 2000 × g, and the supernatant was discarded. Wash three times with 1

show abstract

“…However, it has been found that some volatile chemicals play an important role in this process of PWN exit from the vector. It was found that the monoterpenes released from healthy pine trees, such as β - myrcene and α-pinene, had the strongest attraction to PWN, these chemicals playing an important role in the process of PWN departure from vector and the invasion of healthy host trees, as well as in the movement of PWN within pine trees [21, 22]. Ishkawa et al found that β - myrcene on the agar plate held obvious attraction for dispersive third-stage juveniles [23].…”

Section: Introductionmentioning

confidence: 99%

Effect of choline-phosphate cytidylyltransferase gene (pcyt-1) expression on departure of pine wood nematode,Bursaphelenchus xylophilus(Nematoda: Aphelenchoididae), fromMonochamus alternatus(Coleoptera:Cerambycidae)

Wang

Chen

2020

Preprint

View full text Add to dashboard Cite

show abstract

Behavioral Responses of thePine Wood Nematodeto Terpenes

Cited by 3 publications

References 1 publication

Toward Chemical Ecology of Plant-Parasitic Nematodes: Kairomones, Pheromones, and Other Behaviorally Active Chemical Compounds

Toward Chemical Ecology of Plant-Parasitic Nematodes: Kairomones, Pheromones, and Other Behaviorally Active Chemical Compounds

Study of the departure of pine wood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) from Monochamus alternatus (Coleoptera: Cerambycidae)

Effect of choline-phosphate cytidylyltransferase gene (pcyt-1) expression on departure of pine wood nematode,Bursaphelenchus xylophilus(Nematoda: Aphelenchoididae), fromMonochamus alternatus(Coleoptera:Cerambycidae)

Contact Info

Product

Resources

About