Behavioral, Electroencephalographic, and Histopathologic Effects of a Neuropeptide Isolated From Tityus serrulatus Scorpion Venom in Rats

Carvalho, Fernando; Nencioni, Ana Leonor Abrahão; Lebrun, Ivo; Sandoval, Maria Regina Lopes; Dorce, Valquíria Abrão Coronado

doi:10.1016/s0091-3057(97)00407-3

Cited by 35 publications

(29 citation statements)

References 37 publications

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“…Results of TsTX intrahippocampal injection were previously described and included long electroencephalographic epileptic-like discharges, immobility, orofacial movements and "wet dog shakes" in all animals (14). The effects started five to ten minutes after the injection and persisted for the entire recording period.…”

Section: Electroencephalographic Recordings and Behavioral Observationsmentioning

confidence: 89%

“…Moreover, in vivo studies reveal that it induces electroencephalographic and histopathological alterations (14).…”

Section: Discussionmentioning

confidence: 99%

“…Tityus serrulatus toxins were obtained according to the method described by Carvalho et al (14). Briefly, the venom was extracted with 0.05 M ammonium …”

Section: Tstx Purificationmentioning

confidence: 99%

“…This α-type sodium channel toxin increases the release of glutamate, acetylcholine and GABA from different preparations of nervous tissue (10)(11)(12)(13). When injected in the dorsal hippocampus of rats, it induces behavioral alterations and epileptic discharges besides neurodegeneration (14).…”

Section: Introductionmentioning

confidence: 99%

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Neurotransmitter evaluation in the hippocampus of rats after intracerebral injection of TsTX scorpion toxin

Nencioni¹,

Barreto²,

Lebrun³

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:TsTX is an α-type sodium channel toxin that stimulates the discharge of neurotransmitters from neurons. In the present study we investigated which neurotransmitters are released in the hippocampus after TsTX injection and if they are responsible for electrographic or histopathological effects. Microdialysis revealed that the toxin increased glutamate extracellular levels in the hippocampus; however, levels of gamma-aminobutyric acid (GABA), glycine, 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA) and 3,4-dihydroxyphenylacetic acid (DOPAC) were not significantly altered. Neurodegeneration in pyramidal cells of hippocampus and electroencephalographic alterations caused by the toxin were blocked by pretreatment with riluzole, a glutamate release inhibitor. The present results suggest a specific activity of TsTX in the hippocampus which affects only glutamate release.

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Section: Electroencephalographic Recordings and Behavioral Observationsmentioning

confidence: 89%

“…Moreover, in vivo studies reveal that it induces electroencephalographic and histopathological alterations (14).…”

Section: Discussionmentioning

confidence: 99%

“…Tityus serrulatus toxins were obtained according to the method described by Carvalho et al (14). Briefly, the venom was extracted with 0.05 M ammonium …”

Section: Tstx Purificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Neurotransmitter evaluation in the hippocampus of rats after intracerebral injection of TsTX scorpion toxin

Nencioni¹,

Barreto²,

Lebrun³

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

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show abstract

“…It is known that the scorpion toxins bind to neurotoxin receptor sites blocking sodium channel inactivation or potassium channels. The binding to these channel receptors increases the depolarization time of the channel and consequently, induces excessive neurotransmitter release resulting in the brain damage (9).…”

Section: Discussionmentioning

confidence: 99%

Light and Transmission Electron Microscopical Changes Associated with Leiurus Quinqestriatus Venom in Rabbits

Afifi

El-Kashef

Seddek

et al. 2016

Macedonian Veterinary Review

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Original Scientific ArticleMac Vet Rev 2016; 39 (1): [51][52][53][54][55][56][57] Thirty California female rabbits were obtained from the Animal Care Center, College of Agriculture, South Valley University and acclimated to laboratory conditions for one week. The Leiurus quinquestriatus (LQ) venom was collected from mature scorpions by electrical stimulation of the telson. A single dose of crude venom of 0.4 ml/kg (diluted in normal saline with a ratio of 1:1) was injected into a peripheral ear vein. The lungs, brains, hearts, kidneys, were sampled and fixed in 10% formalin from rabbits sacrificed at zero, 30 minutes, 1hr, and 4hrs, post-envenomation (three animals at each sacrifice). Respiratory distress and neurological manifestations were the main clinical signs. Congestion of the lungs was started at one hour postenvenomation. Vascular changes including hyperemia and hemorrhage were also observed till 24 hours post-envenomation. The main histopathological changes of the lungs were edema, hemorrhage, emphysema, and eosinophilic bronchitis. Transmission electron microscopy revealed several eosinophils with abundant granules and breakdown of their membranes suggesting degranulation. The cerebrum showed malacia and edema. Myocardial damage expressed by focal area of myolysis at half-hour post-envenomation and interstitial edema by at 1, and 4 hour post-envenomation was also evident. In conclusion, scorpion venom induced consistent and relevant histopathological changes in all examined organs.

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TSII toxin isolated from Tityus serrulatus scorpion venom: behavioral, electroencephalographic, and histopathologic studies

Sandoval

Lebrun

2003

Brain Research Bulletin

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Behavioral, Electroencephalographic, and Histopathologic Effects of a Neuropeptide Isolated From Tityus serrulatus Scorpion Venom in Rats

Cited by 35 publications

References 37 publications

Neurotransmitter evaluation in the hippocampus of rats after intracerebral injection of TsTX scorpion toxin

Neurotransmitter evaluation in the hippocampus of rats after intracerebral injection of TsTX scorpion toxin

Light and Transmission Electron Microscopical Changes Associated with Leiurus Quinqestriatus Venom in Rabbits

TSII toxin isolated from Tityus serrulatus scorpion venom: behavioral, electroencephalographic, and histopathologic studies

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