Behavioral effects of temperature sensitive mutations affecting metabolism of cAMP in Drosophila melanogaster

Savvateeva, Elena V.; Kamyshev, N. G.

doi:10.1016/0091-3057(81)90119-2

Cited by 21 publications

(14 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It could be assumed that the perfect learning at 22°C results from calmodulindependent activation of AC, which at 29°C is overcome by the effects of calmodulinactivated PDE-I, capable of hydrolyzing both cAMP and cGMP. This latter fact was proposed earlier [8] to be the cause of the impaired learning ability, since it is cGMP that mediates the effects of acetylcholine, the prevailing neurotransmitter of the insect brain, and the improved learning, at least in ts622, has been assumed to be associated with the increased activity of AC mediating the effects of dopamine, the only neurotransmitter found in the insect brain besides acetylcholine [35]. Since ts622 is a perfect learner at 29°C and is allelic to ts398, mutant calmodulins might have been responsible for more efficient activation of AC by dopamine in both cases of the increased learning ability.…”

Section: Discussionmentioning

confidence: 69%

“…This duration of heat-treatment was shown to be sufficient for altering the behavioral performances in the mutants [8]. As seen in Figure 3, the interstrain differences in PDE activity are preserved through the ontogenetic period studied, the 5th day being the most demonstrative in this respect.…”

Section: Dynamics Of Pde Activity In Adult Fliesmentioning

confidence: 85%

“…As shown earlier [8], PDE in ts398 is more active than in the wild-type while hydrolyzing both cAMP and cGMP, and it is the only mutant among those affecting PDE in which AC activity exceeds that of the wild-type at 22 "C and is readily activated at 29°C [ 101.…”

Section: Temperature-dependence Of Pde Activity In Adult Fliesmentioning

confidence: 97%

See 2 more Smart Citations

Expression of adenylate cyclase and phosphodiesterase in development of temperature‐sensitive mutants with impaired metabolism of cAMP in drosophila melanogaster

et al. 1984

Self Cite

View full text Add to dashboard Cite

The mode of the developmental expression of adenylate cyclase (AC) and phosphodiesterase (PDE) in D melanogaster indicates that PDE plays the major role in the maintenance of a certain level of cAMP in postembryonic development, while both enzymes function in concert in imago. The ts-mutants ts155 and ts622, characterized upon their isolation as having an increased cAMP content and normal PDE activity, manifest high levels of AC activity from the third day of imago life. The levels of PDE activity characteristic for adult mutants with altered enzyme activity (low in ts66 and ts980, high in ts398) are manifested in ts980 from larval instar 11, and from the larval instar 111 in ts398 and ts66. Data on the dependence of PDE activity in adults upon temperature of incubation, being in agreement with the expectations for a ts-mutation in a gene coding for a form of PDE in case of ts66, suggest that ts398 affects not the enzyme-coding gene but rather one for an activator protein. The fact that in ts398 (the polyphasic ts-lethal mapping to 1-38.9) I ) AC activity is somewhat higher than normal at 22°C and is readily activated at 29"C, 2) activity of PDE-I assayed in heat-pretreated homogenates is higher than normal, 3) that boiled extracts of ts398 are potent activators of the wild type and of its own PDE-I indicates that it is a mutation affecting calmodulin, which is known to be stable at boiling and capable of activating both AC and PDE-I. Data on Ca2+ and EGTA effects suggest that the mutation presumably increases Ca2+-binding activity of calmodulin. ts980 and ts622, in which tslethality could be produced only by certain doses of haloperidol and triftazine, appear to be lethal in compounds with ts398, thus indicating that these mutations could affect the same calmodulin-controlling gene.

show abstract

Section: Discussionmentioning

confidence: 69%

Section: Dynamics Of Pde Activity In Adult Fliesmentioning

confidence: 85%

See 1 more Smart Citation

Expression of adenylate cyclase and phosphodiesterase in development of temperature‐sensitive mutants with impaired metabolism of cAMP in drosophila melanogaster

et al. 1984

Self Cite

View full text Add to dashboard Cite

show abstract

“…This is indicated by data ob-tained in studies of Drosophila larvae. Mutations of a single gene controlling the activation properties of the Ca2+/calmodulin receptor protein have pleiotropic influences on excitability [27] and on the cytogenetic properties of the larval brain--chromosomal agglomeration and the mitotic index [23], ectopic contacts of polytene chromosomes [12], and adaptive properties, especially sensitivity to heat shock [14,15], as well as on the learning ability of imagoes [21].…”

Section: Discussionmentioning

confidence: 99%

Proliferative activity and characteristics of the structural-functional organization of the chromosomes in cells of the developing brain, related to the genetically determined excitability of the nervous system in rats

Dyuzhikova

Shiryaeva²,

Vaido³

et al. 2000

Neurosci Behav Physiol

View full text Add to dashboard Cite

This report describes the results of a comparative genetic analysis of the proliferation activity, level of chromosomal aberrations, and the state of interphase chromatin in nerve cells from the developing brains of rat embryos from lines with nervous systems of different excitation thresholds. A relationship between the basal level of nervous system excitability and these cytogenetic characteristics was found. Higher values were seen in embryos of the high-threshold line VP1, as compared with the low-threshold line NP1. The role of the cytogenetic characteristics of the embryo brain in the processes of CNS development and function are discussed in relation to the genetically determined level of nervous system excitability.

show abstract

“…The agn ts3 mutant at this locus displays an unusually high activity of Ca 2+ /calmodulin-dependent phosphodiesterase Pde1 [52]. …”

Section: Drosophila Melanogaster As a Plausible Model To Explore The mentioning

confidence: 99%

Williams Syndrome As a Model for Elucidation of the Pathway Genes - the Brain - Cognitive Functions: Genetics and Epigenetics

Никитина¹,

Medvedeva²,

Захаров³

et al. 2014

Acta Naturae

View full text Add to dashboard Cite

Genomic diseases or syndromes with multiple manifestations arise spontaneously and unpredictably as a result of contiguous deletions and duplications generated by unequal recombination in chromosomal regions with a specific architecture. The Williams syndrome is believed to be one of the most attractive models for linking genes, the brain, behavior and cognitive functions. It is a neurogenetic disorder resulting from a 1.5 Mb deletion at 7q11.23 which covers more than 20 genes; the hemizigosity of these genes leads to multiple manifestations, with the behavioral ones comprising three distinct domains: 1) visuo-spatial orientation; 2) verbal and linguistic defect; and 3) hypersocialisation. The shortest observed deletion leads to hemizigosity in only two genes: eln and limk1. Therefore, the first gene is supposed to be responsible for cardiovascular pathology; and the second one, for cognitive pathology. Since cognitive pathology diminishes with a patient’s age, the original idea of the crucial role of genes straightforwardly determining the brain’s morphology and behavior was substituted by ideas of the brain’s plasticity and the necessity of finding epigenetic factors that affect brain development and the functions manifested as behavioral changes. Recently, non-coding microRNAs (miRs) began to be considered as the main players in these epigenetic events. This review tackles the following problems: is it possible to develop relatively simple model systems to analyze the contribution of both a single gene and the consequences of its epigenetic regulation in the formation of the Williams syndrome’s cognitive phenotype? Is it possible to use Drosophila as a simple model system?

show abstract

Behavioral effects of temperature sensitive mutations affecting metabolism of cAMP in Drosophila melanogaster

Cited by 21 publications

References 17 publications

Expression of adenylate cyclase and phosphodiesterase in development of temperature‐sensitive mutants with impaired metabolism of cAMP in drosophila melanogaster

Expression of adenylate cyclase and phosphodiesterase in development of temperature‐sensitive mutants with impaired metabolism of cAMP in drosophila melanogaster

Proliferative activity and characteristics of the structural-functional organization of the chromosomes in cells of the developing brain, related to the genetically determined excitability of the nervous system in rats

Williams Syndrome As a Model for Elucidation of the Pathway Genes - the Brain - Cognitive Functions: Genetics and Epigenetics

Contact Info

Product

Resources

About