Behavioral effects of acute ethanol in larval zebrafish (D. rerio) depend on genotype and volume of experimental well

Abozaid, Amira; Hung, Joshua Kai Fung; Tsang, Benjamin; Motlana, Keza; Al-Ani, Reem

doi:10.1016/j.pnpbp.2021.110411

Cited by 4 publications

(4 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because Big Al's Aquarium obtains their fish from commercial breeding facilities where the number of breeding pairs is large, we assume that the decreased level of inbreeding meets the above criteria. We have also argued that the increased genetic variability and high heterozygosity ratio makes the WT population more representative of species-typical features of zebrafish found in nature [44,71]. Unlike this genetically heterogeneous population, standard zebrafish strains may have unique, strain-specific idiosyncratic features.…”

Section: Animals and Housingmentioning

confidence: 98%

“…Immobility here is defined as fewer than 20% of the number of pixels corresponding to the total visible surface area of the subject changing from one video-frame to the next (with 30 frame per s temporal resolution). Although intuitively total distance travelled and immobility duration may seem to be redundant, negatively correlating measures, empirical data have suggested that they represent independent behavioral responses [41,44,71,75]. Frequency of immobility (number of immobility episodes) was also quantified as it may represent a behavioral strategy independent of the duration of immobility.…”

Section: Experimental Design and Behavioral Testingmentioning

confidence: 99%

“…Increased thigmotaxis may also be effective in nature as proximity to physical objects (the wall of the tank in the lab or the side of the trunk of an immersed tree in nature) reduces the area of attack by, and makes the prey fish less detectable to, the approaching predator [42]. Indeed, these behavioral measures have been successfully employed to quantify anxiety-like responses and changes in such responses induced in psychopharmacology analyses [43][44][45][46]. We note, however, that anxiety-like responses have been found to represent a rich behavioral repertoire, and how exactly zebrafish respond depends upon the environmental context as well as the nature of the aversive stimulus [39].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Behavioral Effects of Buspirone in Juvenile Zebrafish of Two Different Genetic Backgrounds

Abozaid

2022

Toxics

Self Cite

View full text Add to dashboard Cite

Anxiety continues to represent a major unmet medical need. Despite the availability of numerous anxiolytic drugs, a large proportion of patients do not respond well to current pharmacotherapy, or their response diminishes with chronic drug application. To discover novel compounds and to investigate the mode of action of anxiolytic drugs, animal models have been proposed. The zebrafish is a novel animal model in this research. It is particularly appropriate, as it has evolutionarily conserved features, and drug administration can be employed in a non-invasive manner by immersing the fish into the drug solution. The first step in the analysis of anxiolytic drugs with zebrafish is to test reference compounds. Here, we investigate the effects of buspirone hydrochloride, an anxiolytic drug often employed in the human clinic. We utilize two genetically distinct populations of zebrafish, ABSK, derived from the quasi-inbred AB strain, and WT, a genetically heterogeneous wild-type population. We placed juvenile (10–13-day, post-fertilization, old) zebrafish singly in petri dishes containing one of four buspirone concentrations (0 mg/L control, 5 mg/L, 20 mg/L or 80 mg/L) for 1 h, with each fish receiving a single exposure to one concentration, a between subject experimental design. Subsequently, we recorded the behavior of the zebrafish for 30 min using video-tracking. Buspirone decreased distance moved, number of immobility episodes and thigmotaxis, and it increased immobility duration and turn angle in a quasi-linear dose dependent but genotype independent manner. Although it is unclear whether these changes represent anxiolysis in zebrafish, the results demonstrate that behavioral analysis of juvenile zebrafish may be a sensitive and simple way to quantify the effects of human anxiolytic drugs.

show abstract

Section: Animals and Housingmentioning

confidence: 98%

Section: Experimental Design and Behavioral Testingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Behavioral Effects of Buspirone in Juvenile Zebrafish of Two Different Genetic Backgrounds

Abozaid

2022

Toxics

Self Cite

View full text Add to dashboard Cite

show abstract

“…The consumption of ethanol is an important health concern, and its abuse represents a risk factor for several diseases, such as liver cirrhosis, hypertension, and numerous cancers [ 24 ]. Societal and cultural environmental factors contribute to the consumption of ethanol, as well as genetic factors that predispose one to alcoholism [ 25 ]. In this regard, studies using BXD recombinant inbred (RI) mice have been profitably carried out to investigate and identify genes that are associated with alcohol sensitivity and a predisposition to alcoholism.…”

Section: Ethanolmentioning

confidence: 99%

BXD Recombinant Inbred Mice as a Model to Study Neurotoxicity

et al. 2021

View full text Add to dashboard Cite

BXD recombinant inbred (RI) lines represent a genetic reference population derived from a cross between C57BL/6J mice (B6) and DBA/2J mice (D2), which through meiotic recombination events possesses recombinant chromosomes containing B6 or D2 haplotype segments. The quantitative trait loci (QTLs) are the locations of segregating genetic polymorphisms and are fundamental to understanding genetic diversity in human disease susceptibility and severity. QTL mapping represents the typical approach for identifying naturally occurring polymorphisms that influence complex phenotypes. In this process, genotypic values at markers of known genomic locations are associated with phenotypic values measured in a segregating population. Indeed, BXD RI strains provide a powerful tool to study neurotoxicity induced by different substances. In this review, we describe the use of BXD RI lines to understand the underlying mechanisms of neurotoxicity in response to ethanol and cocaine, as well as metals and pesticide exposures.

show abstract

Experimental arena size alters larval zebrafish photolocomotor behaviors and influences bioactivity responses to a model neurostimulant

Lovin

Scarlett

Henke

et al. 2023

Environment International

View full text Add to dashboard Cite

Behavioral effects of acute ethanol in larval zebrafish (D. rerio) depend on genotype and volume of experimental well

Cited by 4 publications

References 50 publications

Behavioral Effects of Buspirone in Juvenile Zebrafish of Two Different Genetic Backgrounds

Behavioral Effects of Buspirone in Juvenile Zebrafish of Two Different Genetic Backgrounds

BXD Recombinant Inbred Mice as a Model to Study Neurotoxicity

Experimental arena size alters larval zebrafish photolocomotor behaviors and influences bioactivity responses to a model neurostimulant

Contact Info

Product

Resources

About