Experimental arena size alters larval zebrafish photolocomotor behaviors and influences bioactivity responses to a model neurostimulant

Lovin, Lea M.; Scarlett, Kendall R.; Henke, Abigail N.; Sims, Jaylen L.; Brooks, Bryan W.

doi:10.1016/j.envint.2023.107995

Cited by 5 publications

(1 citation statement)

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“…Since locomotor behavior is regarded as a significant indicator of neurodevelopment and is extremely sensitive to environment contaminants, , the light/dark stimulated locomotion of the offspring was examined. Typically, there is a sharp increase or decrease in the swimming speed of zebrafish larvae during light–dark or dark–light transition . However, in this study, we found that the larvae in the 10 μg/L treatments did not show a significant difference in speed in response to light–dark stimulation (Figures G and S2).…”

Section: Resultscontrasting

confidence: 54%

Mechanistic Insights into 1,2-bis(2,4,6-tribromophenoxy)ethane-Induced Male Reproductive Toxicity in Zebrafish

Zheng,

Wang,

Zhang

et al. 2024

Environ. Sci. Technol.

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The novel brominated flame retardant, 1,2-bis(2,4,6tribromophenoxy)ethane (BTBPE), has increasingly been detected in environmental and biota samples. However, limited information is available regarding its toxicity, especially at environmentally relevant concentrations. In the present study, adult male zebrafish were exposed to varying concentrations of BTBPE (0, 0.01, 0.1, 1, and 10 μg/L) for 28 days. The results demonstrated underperformance in mating behavior and reproductive success of male zebrafish when paired with unexposed females. Additionally, a decline in sperm quality was confirmed in BTBPE-exposed male zebrafish, characterized by decreased total motility, decreased progressive motility, and increased morphological malformations. To elucidate the underlying mechanism, an integrated proteomic and phosphoproteomic analysis was performed, revealing a predominant impact on mitochondrial functions at the protein level and a universal response across different cellular compartments at the phosphorylation level. Ultrastructural damage, increased expression of apoptosis-inducing factor, and disordered respiratory chain confirmed the involvement of mitochondrial impairment in zebrafish testes. These findings not only provide valuable insights for future evaluations of the potential risks posed by BTBPE and similar chemicals but also underscore the need for further research into the impact of mitochondrial dysfunction on reproductive health.

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Section: Resultscontrasting

confidence: 54%

Mechanistic Insights into 1,2-bis(2,4,6-tribromophenoxy)ethane-Induced Male Reproductive Toxicity in Zebrafish

Zheng,

Wang,

Zhang

et al. 2024

Environ. Sci. Technol.

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Influence of exposure scenario on the sensitivity to caffeine

Santos,

Oliveira,

Domingues

2023

Environ Sci Pollut Res

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The chorion acts as a protective barrier, restricting some chemical absorption into the embryo and the surrounding fluids. In this sense, larvae may only have direct contact with some chemicals after dechorionation. This study aimed to evaluate the effects of caffeine (CAF) (0, 13, 20, 44, 67, and 100 mg.L−1) under different exposure scenarios (embryos with chorion or embryos/larvae already hatched) and rank the stage sensitivity. Thus, three scenarios were investigated: from 2 to 120 hours post fertilization (hpf) (5 days of exposure- 5dE), from 72 to 120 hpf (2dE), and from 96 to 120 hpf (1dE). Heart rate (48 hpf) and energy reserves (120 hpf) were measured in the 5dE scenario, and behavior and acetylcholinesterase (AChE) activity were evaluated at 120 hpf in all scenarios (5dE, 2dE, and 1dE). At 120 hpf, some of the fish was transferred to clean medium for a 10 days depuration period (10dPE). Behavior and AChE activity were assessed after this period. In the 5dE scenario, CAF increased heartbeat (13, 20, and 30 mg.L−1) and reduced carbohydrates (67, and 100 mg.L−1), while inhibiting AChE activity (100 mg.L−1) in the 5dE, 2dE, and 1dE scenarios. CAF reduced the total distance moved in the 5dE (67, and 100 mg.L−1), 2dE (20, 30, 44, 67, and 100 mg.L−1), and 1dE fish (67, and 100 mg.L−1) and increased erratic movements. Based on the lowest observed effect concentration (LOEC) for total distance moved (20 mg.L−1) and higher inhibition of AChE activity (100 mg.L−1) (65%), 2dE fish appear to be more sensitive to CAF. After 10dPE, a recovery in behavior was detected in all scenarios (5dE, 2dE, and 1dE). AChE activity remained inhibited in the 2dE scenario while increasing in the 1dE scenario. This study demonstrated that the presence of the chorion is an important factor for the analysis of CAF toxicity. After the loss of the chorion, organisms show greater sensitivity to CAF and can be used to evaluate the toxicity of various substances, including nanomaterials or chemicals with low capacity to cross the chorion. Therefore, the use of hatched embryos in toxicity tests is suggested, as they allow a shorter and less expensive exposure scenario that provides similar outcome as the conventional scenario.

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Effects of 4 Testing Arena Sizes and 11 Types of Embryo Media on Sensorimotor Behaviors in Wild-Type and chd7 Mutant Zebrafish Larvae

Hodorovich,

Fryer Harris,

Burton

et al. 2024

Zebrafish

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Experimental arena size alters larval zebrafish photolocomotor behaviors and influences bioactivity responses to a model neurostimulant

Cited by 5 publications

References 71 publications

Mechanistic Insights into 1,2-bis(2,4,6-tribromophenoxy)ethane-Induced Male Reproductive Toxicity in Zebrafish

Mechanistic Insights into 1,2-bis(2,4,6-tribromophenoxy)ethane-Induced Male Reproductive Toxicity in Zebrafish

Influence of exposure scenario on the sensitivity to caffeine

Effects of 4 Testing Arena Sizes and 11 Types of Embryo Media on Sensorimotor Behaviors in Wild-Type and chd7 Mutant Zebrafish Larvae

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