Behavioral Characterization of<i>dmrt3a</i>Mutant Zebrafish Reveals Crucial Aspects of Vertebrate Locomotion through Phenotypes Related to Acceleration

Pozo, Ana del; Manuel, Remy; González, Ana Belén Iglesias; Koning, Harmen Kornelis; Habicher, Judith; Zhang, Hanqing; Allalou, Amin; Kullander, Klas; Boije, Henrik

doi:10.1523/eneuro.0047-20.2020

Cited by 18 publications

(23 citation statements)

References 40 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our analysis, only acceleration of the larval fish was reduced following DINCH exposure. The study by Del Pozzo et al., showed that dmrt3a gene knockout zebrafish larvae has reduced movement and acceleration due to loss of coordination in the spinal interneurons ( Del Pozo et al., 2020 ). This suggests that apart from distance, acceleration is also an important parameter to consider for behavioral or neurological changes.…”

Section: Discussionmentioning

confidence: 99%

“…Expression of dmrt3a is associated with spinal cord development and fate specification of dorsal interneurons 6 that coordinates locomotion in animals ( Andersson et al., 2012 ). In a recent study, it was demonstrated that dmrt3a -knockout zebrafish has altered behavior with decreased movement, and acceleration ( Del Pozo et al., 2020 ). We observed an increase in acceleration and parallel with this, significantly induced expression of dmrt3a .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) alters transcriptional profiles, lipid metabolism and behavior in zebrafish larvae

Saad

Bereketoğlu

Pradhan

2021

Heliyon

View full text Add to dashboard Cite

Plasticizers are commonly used in different consumer goods and personal care products to provide flexibility, durability and elasticity to polymers. Due to their reported toxicity, the use of several plasticizers, including phthalates has been regulated and/or banned from the market. Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) is an alternative plasticizer that was introduced to replace toxic plasticizers. Increasing global demand and lack of toxicity data and safety assessment of DINCH have raised the concern to human and animal health. Hence, in the present study, we investigated the adverse effects of DINCH (at concentrations ranging from 0.01 to 10 μM) in early developmental stages of zebrafish using different endpoints such as hatching rate, developmental abnormalities, lipid content, behavior analysis and gene expression. We found that DINCH caused hatching delay in a dose-dependent manner and altered the expression of genes involved in stress response. Lipid staining using Oil Red O stain showed a slight lipid accumulation around the yolk, brain, eye and neck with increasing concentration. Genes associated with lipid transport such as fatty acid synthesis, β-oxidation, elongation, lipid transport were significantly altered by DINCH. Genes involved in cholesterol biosynthesis and homeostasis were also affected by DINCH indicating possible developmental neurotoxicity. Behavioral analysis of larvae demonstrated a distinct locomotor activity upon exposure to DINCH. The present data shows that DINCH could induce physiological and metabolic toxicity to aquatic organisms. Hence, further analyses and environmental monitoring of DINCH should be conducted to determine its safety and toxicity levels.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) alters transcriptional profiles, lipid metabolism and behavior in zebrafish larvae

Saad

Bereketoğlu

Pradhan

2021

Heliyon

View full text Add to dashboard Cite

show abstract

“…A premature stop codon by DMRT3 mutation in horses changes locomotion patterns, which is favorable for harnessing racing performances ( Andersson et al, 2012 ; Perry et al, 2019 ). Zebrafish studies have also reported that Dmrt3-expressing neurons contribute to locomotor activity ( Del Pozo et al, 2020 ; Satou et al, 2020 ). These results suggest a conserved function of Dmrt3 in the spinal cord of vertebrates.…”

Section: Functions Of Dmrt Genes In the Specification Of Neural Stem/progenitor Cells And Neurons Outside The Developimentioning

confidence: 99%

Multiple Functions of the Dmrt Genes in the Development of the Central Nervous System

Kikkawa

Osumi

2021

Front. Neurosci.

View full text Add to dashboard Cite

The Dmrt genes encode the transcription factor containing the DM (doublesex and mab-3) domain, an intertwined zinc finger-like DNA binding module. While Dmrt genes are mainly involved in the sexual development of various species, recent studies have revealed that Dmrt genes, which belong to the DmrtA subfamily, are differentially expressed in the embryonic brain and spinal cord and are essential for the development of the central nervous system. Herein, we summarize recent studies that reveal the multiple functions of the Dmrt genes in various aspects of vertebrate neural development, including brain patterning, neurogenesis, and the specification of neurons.

show abstract

“…All zebrafish larvae were collected, housed, and treated as previously described by del Pozo at el. in [23]. Pigmentation in larvae used for in situ hybridization was inhibited by addition of 0.003% 1-Phenyl-2-thiourea (PTU) to the embryo water.…”

Section: Sample Preparationmentioning

confidence: 99%

zOPT: an open source optical projection tomography system and methods for rapid 3D zebrafish imaging

Zhang¹,

Waldmann²,

Manuel³

et al. 2020

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

Optical projection tomography (OPT) is a 3D imaging alternative to conventional microscopy which allows imaging of millimeter-sized object with isotropic micrometer resolution. The zebrafish is an established model organism and an important tool used in genetic and chemical screening. The size and optical transparency of the embryo and larva makes them well suited for imaging using OPT. Here, we present an open-source implementation of an OPT platform, built around a customized sample stage, 3D-printed parts and open source algorithms optimized for the system. We developed a versatile automated workflow including a two-step image processing approach for correcting the center of rotation and generating accurate 3D reconstructions. Our results demonstrate high-quality 3D reconstruction using synthetic data as well as real data of live and fixed zebrafish. The presented 3D-printable OPT platform represents a fully open design, low-cost and rapid loading and unloading of samples. Our system offers the opportunity for researchers with different backgrounds to setup and run OPT for large scale experiments, particularly in studies using zebrafish larvae as their key model organism.

show abstract

Behavioral Characterization ofdmrt3aMutant Zebrafish Reveals Crucial Aspects of Vertebrate Locomotion through Phenotypes Related to Acceleration

Cited by 18 publications

References 40 publications

Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) alters transcriptional profiles, lipid metabolism and behavior in zebrafish larvae

Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) alters transcriptional profiles, lipid metabolism and behavior in zebrafish larvae

Multiple Functions of the Dmrt Genes in the Development of the Central Nervous System

zOPT: an open source optical projection tomography system and methods for rapid 3D zebrafish imaging

Contact Info

Product

Resources

About