Behavioral Changes in Stem-Cell Potency by HepG2-Exhausted Medium

Balzano, Francesca; Garroni, Giuseppe; Cruciani, Sara; Bellu, Emanuela; Giudici, Silvia Dei; Oggiano, Annalisa; Capobianco, Giampiero; Dessole, Salvatore; Ventura, Carlo; Maioli, Margherita

doi:10.3390/cells9081890

Cited by 7 publications

(6 citation statements)

References 62 publications

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“…Mesenchymal stem cells can differentiate into different cell lineages upon stimulation. This ability is closely related to achieving a perfect balance between the pluripotency-related genes (which control stem cell proliferation) and genes that can orchestrate the appearance of a specific phenotype [ 40 ]. In this regard, adipogenesis and osteoblastogenesis (adipo–osteoblastogenesis) are closely related processes [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Vitamin D ameliorates insulin resistance-induced osteopenia by inactivating the nucleotide-binding oligomerization domain-like receptor protein 3 inflammasome

Wu¹,

Cai²,

Yang³

et al. 2023

Heliyon

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Section: Discussionmentioning

confidence: 99%

Vitamin D ameliorates insulin resistance-induced osteopenia by inactivating the nucleotide-binding oligomerization domain-like receptor protein 3 inflammasome

Wu¹,

Cai²,

Yang³

et al. 2023

Heliyon

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“…Nevertheless, some issues are still open. In particular, the clear evidence that stem cell behavior and fate are influenced by the cellular microenvironment [ 5 , 6 , 7 , 10 , 37 ] raises more than a cautionary note on the use of a stem cell source in patients who underwent mastectomy as a part of a breast cancer treatment.…”

Section: Discussionmentioning

confidence: 99%

“…It is worth considering that the overexpression of these genes could be associated with the initiation of an uncontrolled transformation leading to a malignant phenotype, as it has been documented elsewhere [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

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Adipose-Derived Stem Cell Features and MCF-7

Garroni

Balzano

Cruciani

et al. 2021

Cells

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Human adipose tissue-derived stem cells (hADSCs) are highly suitable for regeneration therapies being easily collected and propagated in vitro. The effects of different external factors and culturing conditions are able to affect hADSC proliferation, senescence, differentiation, and migration, even at the molecular level. In the present paper, we exposed hADSCs to an exhausted medium from the breast cancer cell line (MCF-7) to evaluate whether the soluble factors released by these cells may be able to induce changes in stem cell behavior. In particular, we investigated the expression of stemness-related genes (OCT4; Sox 2; Nanog), the cell-cycle regulators p21 (WAF1/CIP1) p53, epigenetic markers (DNMT1 and Sirt1), and autophagy-related proteins. From our results, we can infer that the exhausted medium from MCF-7 is able to influence the hADSCs behavior increasing the expression of stemness-related genes, cell proliferation, and autophagy. Polyamines detectable in MCF-7 exhausted medium could be related to the higher proliferation capability observed in hADSCs, suggesting direct crosstalk between these molecules and the observed changes in stem cell potency.

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“…The tumour microenvironment and the crosstalk between the epithelial niche and mesenchymal cells seem to be involved in the development of PC. The exhausted medium by human HepG2 and MCF7 cell lines can modulate stem cell fate by direct activating specific epigenetic modifications, increasing the expression of stemness-related genes, cell proliferation, and autophagy [67,68]. Genetic manipulation of stromal cells can lead to tumour formation, as well as the possible reprogramming of adult differentiated cells to an embryonic-like state, indicating that the microenvironment is a key player in the control of oncogenic cell signals and in the generation of cancerous conditions [69].…”

Section: Upregulated Mir-185mentioning

confidence: 99%

Role of miRNA-145, 148, and 185 and Stem Cells in Prostate Cancer

Coradduzza

Cruciani

Arru

et al. 2022

IJMS

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MicroRNAs (miRNAs) are small non-coding RNA molecules that play a role in cancer linked to the regulation of important cellular processes and pathways involving tumorigenesis, cell proliferation, differentiation, and apoptosis. A lot of human miRNA sequences have been identified which are linked to cancer pathogenesis. MicroRNAs, in prostate cancer (PC), play a relevant role as biomarkers, show a specific profile, and have been used as therapeutic targets. Prostate cancer (PC) is the most frequently diagnosed cancer in men. Clinical diagnoses among the gold standards for PC diagnosis and monitoring are prostate-specific antigen (PSA) testing, digital rectal examination, and prostate needle biopsies. PSA screening still has a large grey area of patients, which leads to overdiagnosis. Therefore, new biomarkers are needed to improve existing diagnostic tools. The miRNA expression profiles from tumour versus normal tissues are helpful and exhibit significant differences not only between cancerous and non-cancerous tissues, but also between different cancer types and subtypes. In this review, we focus on the role of miRNAs-145, 148, and 185 and their correlation with stem cells in prostate cancer pathogenesis. MiR-145, by modulating multiple oncogenes, regulates different cellular processes in PC, which are involved in the transition from localised to metastatic disease. MiR-148 is downregulated in high-grade tumours, suggesting that the miR-148-3 family might act as tumour suppressors in PC as a potential biomarker for detecting this disease. MiR-185 regulation is still unclear in being able to regulate tumour processes in PC. Nevertheless, other authors confirm the role of this miRNA as a tumour suppressor, suggesting its potential use as a suitable biomarker in disease prognosis. These three miRNAs are all involved in the regulation of prostate cancer stem cell behaviour (PCSCs). Within this contest, PCSCs are often involved in the onset of chemo-resistance in PC, therefore strategies for targeting this subset of cells are strongly required to control the disease. Hence, the relationship between these two players is interesting and important in prostate cancer pathogenesis and in PCSC stemness regulation, in the attempt to pave the way for novel therapeutic targets in prostate cancer.

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Behavioral Changes in Stem-Cell Potency by HepG2-Exhausted Medium

Cited by 7 publications

References 62 publications

Vitamin D ameliorates insulin resistance-induced osteopenia by inactivating the nucleotide-binding oligomerization domain-like receptor protein 3 inflammasome

Vitamin D ameliorates insulin resistance-induced osteopenia by inactivating the nucleotide-binding oligomerization domain-like receptor protein 3 inflammasome

Adipose-Derived Stem Cell Features and MCF-7

Role of miRNA-145, 148, and 185 and Stem Cells in Prostate Cancer

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