Vitamin D ameliorates insulin resistance-induced osteopenia by inactivating the nucleotide-binding oligomerization domain-like receptor protein 3 inflammasome

Wu, Min; Cai, Yong; Yang, Yan; Hu, Hao-Ming; Yang, Yue; Jia, Yingmin; Deng, Jia-Jie; Wan, Liu

doi:10.1016/j.heliyon.2023.e13215

Cited by 3 publications

(1 citation statement)

References 48 publications

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“…Arachidonic acid, metabolized into pro-inflammatory eicosanoids during an inflammatory response, is recognized as an inflammatory mediator [ 38 ]. Furthermore, type 2 diabetes is categorized as an inflammatory disease [ 39 ]. Given our previous results demonstrating tocotrienol’s anti-inflammatory capacity, it can be inferred that tocotrienol may exert an anti-inflammatory function by reducing the metabolism of arachidonic acid [ 4 ].…”

Section: Discussionmentioning

confidence: 99%

Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet

Liu,

Zhang,

Xue

et al. 2024

IJMS

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In recent years, the potent influence of tocotrienol (T3) on diminishing blood glucose and lipid concentrations in both Mus musculus (rats) and Homo sapiens (humans) has been established. However, the comprehensive exploration of tocotrienol’s hypolipidemic impact and the corresponding mechanisms in aquatic species remains inadequate. In this study, we established a zebrafish model of a type 2 diabetes mellitus (T2DM) model through high-fat diet administration to zebrafish. In the T2DM zebrafish, the thickness of ocular vascular walls significantly increased compared to the control group, which was mitigated after treatment with T3. Additionally, our findings demonstrate the regulatory effect of T3 on lipid metabolism, leading to the reduced synthesis and storage of adipose tissue in zebrafish. We validated the expression patterns of genes relevant to these processes using RT-qPCR. In the T2DM model, there was an almost two-fold upregulation in pparγ and cyp7a1 mRNA levels, coupled with a significant downregulation in cpt1a mRNA (p < 0.01) compared to the control group. The ELISA revealed that the protein expression levels of Pparγ and Rxrα exhibited a two-fold elevation in the T2DM group relative to the control. In the T3-treated group, Pparγ and Rxrα protein expression levels consistently exhibited a two-fold decrease compared to the model group. Lipid metabolomics showed that T3 could affect the metabolic pathways of zebrafish lipid regulation, including lipid synthesis and decomposition. We provided experimental evidence that T3 could mitigate lipid accumulation in our zebrafish T2DM model. Elucidating the lipid-lowering effects of T3 could help to minimize the detrimental impacts of overfeeding in aquaculture.

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Section: Discussionmentioning

confidence: 99%

Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet

Liu,

Zhang,

Xue

et al. 2024

IJMS

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Redox Regulation of Nucleotide-Binding and Oligomerization Domain-Like Receptors Inflammasome

Ferrara

Pecorelli

Valacchi

2023

Antioxidants & Redox Signaling

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Influence of METTL3 knockdown on PDLSC osteogenesis in E. coliLPS‐induced inflammation

Chen,

Peng,

Wang

et al. 2023

Oral Diseases

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ObjectiveThis study aimed to investigate the effect of METTL3 knockdown on osteogenic differentiation of human periodontal ligament stem cells (PDLSCs) in the weak inflammation microenvironments, as well as the underlying mechanisms.Materials and MethodsPDLSCs were stimulated by lipopolysaccharide from Escherichia coli (E. coli LPS), followed by quantification of METTL3. METTL3 expression was assessed using RT‐qPCR and Western blot analysis in periodontitis. METTL3 knockdown PDLSCs were stimulated with or without E. coli LPS. The evaluation included proinflammatory cytokines, osteogenic markers, ALP activity, and mineralized nodules. Bioinformatics analysis and Western blot determined the association between METTL3 and the PI3K/Akt pathway.ResultsMETTL3 was overexpressed in periodontitis. METTL3 knockdown in PDLSCs reduced proinflammatory cytokines, osteogenic markers, ALP activity, and mineralized nodules in both environments. Bioinformatics analysis suggested a link between METTL3 and the PI3K/Akt pathway. METTL3 knockdown inhibited PI3K/Akt signaling pathway activation.ConclusionMETTL3 knockdown might inhibit osteogenesis in PDLSCs through the inactivation of PI3K/Akt signaling pathway. Concomitant findings might shed novel light on the roles and potential mechanisms of METTL3 in the LPS‐stimulated inflammatory microenvironments of PDLSCs.

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Vitamin D ameliorates insulin resistance-induced osteopenia by inactivating the nucleotide-binding oligomerization domain-like receptor protein 3 inflammasome

Cited by 3 publications

References 48 publications

Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet

Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet

Redox Regulation of Nucleotide-Binding and Oligomerization Domain-Like Receptors Inflammasome

Influence of METTL3 knockdown on PDLSC osteogenesis in E. coliLPS‐induced inflammation

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