Behavioral and Pharmacological Aspects of Anxiety in the Light/Dark Preference Test

Araújo, Juliana Baldan Costa Neves; Maximino, Caio; Brito, Thiago Marques; Silva, Annanda Waneza Batista da; Oliveira, Karen Renata Matos; Batista, Evander de Jesus Oliveira; Morato, Sílvio; Herculano, Anderson Manoel; Gouvêia, Amauri

doi:10.1007/978-1-61779-597-8_14

Cited by 14 publications

(10 citation statements)

References 30 publications

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“…Determination of drug effects on scototaxis was carried as described elsewhere (Araújo et al 2012). Briefly, after drug injection and effect onset animals were transferred to a black and white central compartment of a black and white tank (15 × 10 × 45 cm, height × depth × length) for a 3-min acclimation period, after which the doors which delimit this compartment were removed and the animal was allowed to freely explore the apparatus for 15 min.…”

Section: Light-dark Preference (Scototaxis)mentioning

confidence: 99%

Behavioral and neurochemical changes in the zebrafish leopard strain

Maximino¹,

Puty

Oliveira

et al. 2013

Genes Brain and Behavior

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The zebrafish leopard phenotype (leo) displays abnormal pigmentation and shows increased anxiety-like behavior. The neurochemical changes associated with this anxious phenotype are not known. Here, we demonstrate that leo show increased anxiety-like behavior in the light/dark box and in the novel tank test. This anxious phenotype is rescued by acute treatment with a dose of a serotonin reuptake inhibitor, fluoxetine, that is inactive in wild-type animals. Moreover, leo show decreased tissue levels of serotonin, increased serotonin turnover and slightly increased monoamine oxidase activity. These results suggest that the anxious phenotype observed in leo zebrafish is caused by a decrease in serotonin uptake. This work could open an important avenue in defining the neurochemical underpinning of natural variation in anxiety disorders.

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Section: Light-dark Preference (Scototaxis)mentioning

confidence: 99%

Behavioral and neurochemical changes in the zebrafish leopard strain

Maximino¹,

Puty

Oliveira

et al. 2013

Genes Brain and Behavior

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“…The LD paradigm is a sensitive test of disorders involving generalized anxiety (Araujo et al, 2012). The LD box consists of a light compartment (27×27×27 cm) and a dark compartment (black walls and floor, 27×18×27 cm) separated by a single partition with an opening (7×7 cm), which allowed passage between the two compartments (Salim, et al, 2010).…”

Section: Methodsmentioning

confidence: 99%

Regular exercise prevents non-cognitive disturbances in a rat model of Alzheimer's disease

Dao

Zagaar

Salim

et al. 2013

Int. J. Neuropsychopharm.

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Previously, we reported that in a rat model of sporadic Alzheimer’s disease (AD) generated by exogenous administration of Aβ1–42 (250 pmol/d for 2 wk) via mini-osmotic pump, the animals exhibited learning and memory impairment, which could be attributed to the deleterious alterations in the levels of cognition-related signalling molecules. We showed that 4 wk of treadmill exercise totally prevented these impairments. Here, we evaluated the effect of exercise on non-cognitive function and basal synaptic transmission in the Cornu Ammonis 1 (CA1) area using the same AD model. Our results indicated that the anxiety behaviour of Aβ-treated rats was prevented by 4 wk of treadmill exercise. Exercised/Aβ-infused rats spent a longer time in the centre area of the open field (OF), elevated plus maze (EPM) paradigms and the light area of the light-dark (LD) box, which were similar to those of control and exercise rats. Furthermore, under basal conditions the aberrant up-regulation of calcineurin (PP2B) and reduction of phosphorylated Ca2+/calmodulin dependent protein kinase II (p-CaMKII) levels induced by AD-like pathology were normalised by the exercise regimen. We conclude that regular exercise may exert beneficial effects on both cognitive and non-cognitive functions in this AD model.

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“…The typical LDT apparatus is a rectangular tank consisting of two equal vertical portions, black and white (Maximino, De Brito, et al, ). In this test, anxiogenic‐like effects include more time spent in the dark (Maximino, De Brito, et al, ), in addition to risk assessment (defined as partial or very fast entries in the white compartment, during which the animal gathers information on threat levels), thigmotaxis (swimming near the walls of the tank), freezing (cessation of swimming and most movements), and erratic swimming (a zig‐zagging, fast pattern of swimming in which the animal's direction is unpredictable to predators) in the white area (Araujo et al, ). Interestingly, the characterization of anxiety in the LDT paradigm is age‐specific, as for instance, larval fish display natural dark avoidance (Steenbergen, Richardson, & Champagne, ) that is attenuated by anxiolytic (e.g., diazepam, buspirone or ethanol) and increased by anxiogenic (e.g., caffeine) drugs (Steenbergen et al, ).…”

Section: Measuring Affective‐like Behavior In Crayfish and Zebrafishmentioning

confidence: 99%

Emotional behavior in aquatic organisms? Lessons from crayfish and zebrafish

Abreu

Maximino

Banha

et al. 2019

J of Neuroscience Research

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Experimental animal models are a valuable tool to study the neurobiology of emotional behavior and mechanisms underlying human affective disorders. Mounting evidence suggests that various aquatic organisms, including both vertebrate (e.g., zebrafish) and invertebrate (e.g., crayfish) species, may be relevant to study animal emotional response and its deficits. Ideally, model organisms of disease should possess considerable genetic and physiological homology to mammals, display robust behavioral and physiological responses to stress, and should be sensitive to a wide range of drugs known to modulate stress and affective behaviors. Here, we summarize recent findings in the field of zebrafish‐ and crayfish‐based tests of stress, anxiety, aggressiveness and social preference, and discuss further perspectives of using these novel model organisms in translational biological psychiatry. Outlining the remaining questions in this field, we also emphasize the need in further development and a wider use of crayfish and zebrafish models to study the pathogenesis of affective disorders.

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Behavioral and Pharmacological Aspects of Anxiety in the Light/Dark Preference Test

Cited by 14 publications

References 30 publications

Behavioral and neurochemical changes in the zebrafish leopard strain

Behavioral and neurochemical changes in the zebrafish leopard strain

Regular exercise prevents non-cognitive disturbances in a rat model of Alzheimer's disease

Emotional behavior in aquatic organisms? Lessons from crayfish and zebrafish

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