Behavior of the c-reactive protein during purification and electrophoretic characterization

Riley, Ralph; Coleman, Monroe; Hokama, Y.

doi:10.1016/0009-8981(65)90009-4

Cited by 10 publications

(5 citation statements)

References 11 publications

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“…Examination of purified CRP by free boundary electrophoresis showed the presence of a y-mobility protein (46). On the other hand, analysis by zonal electrophoresis using specific antibody to CRP has shown CRP with a, b, and y mobilities (71,85,(101)(102)(103)(104)(105)(106)(107)(108). This variability in mobility in semisolid media has been attributed, in part, to binding of CRP to the medium in the presence of divalent ions, to the nature of antibody to CRP, and to its possible binding with impurities in the preparations (75,85,102).…”

Section: Source Of Crp and Normal Valuesmentioning

confidence: 96%

“…The simple capillary tube procedure used extensively in earlier studies using C-plysaccharide or CRPA as the reagent tends to yield inconsistent results. This has been attributed in part to different heterologous CRP antibody preparations, contaminated reagents (unrecognized anti-SAP), frozen semm samples (CRP tends to form subunits with freezing and thawing), and bacterial contaminants (84)(85)(86).…”

Section: --mentioning

confidence: 99%

“…On the other hand, analysis by zonal electrophoresis using specific antibody to CRP has shown CRP with a, b, and y mobilities (71,85,(101)(102)(103)(104)(105)(106)(107)(108). This variability in mobility in semisolid media has been attributed, in part, to binding of CRP to the medium in the presence of divalent ions, to the nature of antibody to CRP, and to its possible binding with impurities in the preparations (75,85,102). With the lack of appropriate standardization of the antisera and purified CRP, some of the reported patterns in the a and particularly the p region in agar gel immunoelectrophoresis may be attributed to serum amyloid P component, which is closely related to CRP ( 6 2 4 6 ) .…”

Section: Source Of Crp and Normal Valuesmentioning

confidence: 99%

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C‐Reactive protein: Current status and future perspectives

Hokama

Nakamura

1987

Clinical Laboratory Analysis

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This review encompasses all aspects of re-Reactive protein analysis relative to simplicity cent C-Reactive protein studies. Emphasis has and sensitivity. The responses of C-Reactive been in the areas of (1) the physical, chemical, protein in various diseases and its future prosand potential functions of C-Reactive protein; pects are also discussed in this review. and (2) the pass and recent procedures of C-

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Section: Source Of Crp and Normal Valuesmentioning

confidence: 96%

Section: --mentioning

confidence: 99%

Section: Source Of Crp and Normal Valuesmentioning

confidence: 99%

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C‐Reactive protein: Current status and future perspectives

Hokama

Nakamura

1987

Clinical Laboratory Analysis

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“…In this regard a preliminary report (50) showed 63% inhibition of fluorescein labeled CRP binding to monocyte by tuftsin. An alternate explanation may be ingestion of the CRP-PAF or other CRP complexes by neutrophils and subsequent degradation of CRP to its active subunits or neoantigens (30,31,45). The latter then activates macrophages to produce its monokines (30,31,32).…”

Section: Il-1 Mouse Thymocyte Assay Of Supernatants Of Peripheral Blomentioning

confidence: 98%

C‐reactive protein and platelet activating factor complexes induce production and release of interleukin‐1 by human monocytes

Hokama

Honda

Terada

1988

Clinical Laboratory Analysis

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Stimulation of interleukin‐1 (IL‐1) in peripheral blood monocytes exposed to C‐reactive protein (CRP) and C‐reactive protein‐platelet‐activating factor (CRP‐PAF) complexes was demonstrated in vitro in this study. Significant synthesis of intracellular IL‐1, 2‐5‐fold increases in the number of adherent monocytes as determined by indirect immunofluorescence using rabbit anti‐IL‐1 and goat antirabbit IgG‐fluorescein isothiocynate, was shown. Both CRP alone (10 μg/ml) and especially CRP‐PAF mixture (10 μg CRP/1 ug PAF/ml) showed maximum stimulation of IL‐1 of 41 and 95 cells/10 field examined, respectively. CRP alone examined in a dose‐response study showed optimum stimulation of IL‐1 production at the 1 μg/ml concentration with 57% of monocytes showing fluorescence. CRP at varying concentrations with a constant amount of PAF (1 μg/ml) showed optimum IL‐1 staining monocytes (63%) with CRP at 10 μg/ml (10 pg CRP/l μg PAF). Similar significant enhanced extracellular IL‐1 production by monocytes exposed to CRP and CRP/PAF mixtures was shown by the mouse thymocyte assay. Supernatants of monocytes exposed to CRP (10 μg/ml) or CRP‐PAF (10 μg/l μg/ml) demonstrated mouse IL‐1 synthesis of 92 and 110 stimulation indices in the thymocyte assay, respectively. It is suggested that CRP and especially CRP‐PAF complexes activate macrophage through their appropriate receptors to production of monokines which modulate the immune system. Thus, CRP appears to function nonspecifically in a behavior reminiscent of specific immunoglobulins.

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“…The presence in serum 100 A. KOJ of a pentameric form as well was suggested by an asymmetric elution pattern on gel filtration. Riley et al 263 demonstrated that CRP may dissociate during isolation and purification. The approximate m.w.…”

Section: C-reactive Proteinmentioning

confidence: 99%

Structure and Function of Plasma Proteins

Allison¹

1974

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Lipoproteins are macromolecules in which the water insoluble lipids are transported in plasma. Four main lipoprotein classes have been defined in human plasma. Each of these contains esterified and nonesterified cholesterol, phospholipid and triglyceride, though in differing and characteristic proportions. Non-esterified fatty acid (NEF A) is transported as a complex with albumin; the albumin-fatty acid complex is not, however, conventionally regarded as one of the lipoproteins. The terminology used for the classification of the lipoprotein classes depends upon the methods used for their separation; a description of the methodology is thus a prerequisite to any discussion of terminology or physiology.

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Behavior of the c-reactive protein during purification and electrophoretic characterization

Cited by 10 publications

References 11 publications

C‐Reactive protein: Current status and future perspectives

C‐Reactive protein: Current status and future perspectives

C‐reactive protein and platelet activating factor complexes induce production and release of interleukin‐1 by human monocytes

Structure and Function of Plasma Proteins

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