Behavior of ingested concanavalin A in the gastrointestinal tract of the rat.

Nakata, Shinobu; Kimura, Tatsuo

doi:10.1271/bbb1961.50.645

Cited by 6 publications

(3 citation statements)

References 2 publications

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“…Con A is highly resistant to proteolysis in vivo and is stable during its passage through the gastrointestinal tract ( Nakata and Kimura 1985). At high levels in the diet it disturbs brush border membrane function and interferes with reformation of brush border membranes ( Nakata and Kimura 1986). There may also be some cytotoxic effects ( Lorenz‐Meyer et al .…”

Section: Discussionmentioning

confidence: 99%

Modulation of Salmonella infection by the lectins of Canavalia ensiformis (Con A) and Galanthus nivalis (GNA) in a rat model in vivo

Naughton¹,

Grant²,

Bardócz³

et al. 2000

J Appl Microbiol

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The plant lectins, Concanavalin A (Con A) and Galanthus nivalis agglutinin (GNA) have been prefed to rats for 3 d pre‐ and 6 d postinfection with Salmonella typhimurium S986 or Salm. enteritidis 857. Con A significantly increased numbers of Salm. typhimurium S986 in the large intestine and in faeces, and severely impaired growth of the rats, more severely than is the case of infection with Salmonella typhimurium alone. Con A had much less effect on rats infected with Salm. enteritidis 857 only showing a significant increase in numbers in the colon, accompanied by intermittent increases of Salmonella in the faeces during the study. GNA significantly reduced pathogen numbers in the lower part of the small bowel and the large intestine of rats infected with Salm. typhimurium S986 and significantly improved rat growth. GNA had little effect on infection by Salm. enteritidis 857 with slight decreases in Salmonella numbers in the small intestine and large intestine and transient increases in the faeces.

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Section: Discussionmentioning

confidence: 99%

Modulation of Salmonella infection by the lectins of Canavalia ensiformis (Con A) and Galanthus nivalis (GNA) in a rat model in vivo

Naughton¹,

Grant²,

Bardócz³

et al. 2000

J Appl Microbiol

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“…Non-nutritious materials such as dietary fiber were added to HSD without replacing any components of the basal diet. The preparation procedures for these materials added to HSD were as follows: (I) Gobo (Arctium lappa L.) dietary fiber (GDF) was prepared in a manner similar to that described in the previous study9); (2) rice bran was defatted by ethyl ether extraction, autoclaved at 120°C for 20 min, and dried by heating; (3) carrot was boiled, homogenized, dehydrated by centrifugation, and finely ground after lyophilizing. These last two resulting residues are hereafter referred as bran dietary fiber (BDF) and carrot dietary fiber (CDF), respectively.…”

Section: Methodsmentioning

confidence: 99%

Effects of Dietary Fiber on Fecal Concanavalin A-binding Glycoprotein in Rats

Nakata

Kimura

1992

Bioscience, Biotechnology, and Biochemistry

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This study investigates the relationship between the dietary components and quantitative changes in fecal concanavalin A-binding glycoprotein (CBGP) derived from small intestinal epithelia in rats. Although fecal CBGP increased with increasing protein content in the diet, the increase in fecal CBGP due to dietary protein was much smaller than that resulting from the addition of pectin, cellulose or Gobo dietary fiber (GDF) to the diet. The increasing effect of 10% GDF by weight added to a 200/0 casein high-sucrose diet (HSD) on fecal CBGP was the most pronounced without producing any gastrointestinal disorders, whereas that from the addition of pectin to HSD at a level of 5% significantly decreased the intestinal sucrase activity. A similar increase in fecal CBGP was observed when rice bran dietary fiber, carrot dietary fiber, Hiziki or Wakame was added to HSD, but these increasing effects on fecal CBGP were independent of their insoluble-soluble ratio. This and our previous observations suggest that such dietary fiber may prompt the renewal and exfoliation of small intestinal epithelia.In the previous studies,1,2) we suggested the presence of a concanavalin A-binding glycoprotein (CBGP) in the gastrointestinal tract and feces of rats. Afterwards, CBGP could be isolated from the stomach, small intestine, cecum, and feces, and was found to be at a higher level in the small intestine than in the'others 3 ) and to be localized in its luminal surface. We recently found that CBGP could be isolated from both the feces of germ-free rats and of the conventionalized rats, but not from the intestinal microflora.4 ) Therefore, the origin of the fecal CBGP was considered to be the luminal surface, i.e. the epithelium, of the small intestine. On the other hand, it has recently become clear that the mucosa of the small intestine is very sensitive to various nutritional conditions 5 ,6); the rate of cell proliferation and the morphology of the villi are influenced by dietary components, and especially by unabsorbed polysaccharides. Accordingly, quantitative changes in fecal CBGP that reflect changes in the exfoliation and renewal of epithelial cells in the small intestine may serve as a criterion for representing the relationship between ingested food components and the luminal surface of the small intestine. Thus, this present study was undertaken to observe the relationship between quantitative changes in fecal CBGP and dietary fiber intake, and to investigate the effect of dietary components on the renewal of epithelia in the small intestine. Materials and MethodsAnimals and diets. Male rats of the Wi star strain weighing approximately 100 g were obtained from Japan SLC, Shizuoka, Japan. They were individually housed in suspended wire-mesh cages in a room kept at 21 to 23°C, with lighting regulated to automatically provide a 12-h light and dark cycle (lighting-up between 8:00 and 20:00). The rats were fed on a basal diet ad libitum for 7 days before the start of the experiments. The composition of the basal diet (HSD) was a...

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“…(1) SI, the fraction from the small intestine; CE, the fraction from the cecum; LI, the fraction from the colon and rectum. (2) F, the fraction from feces.…”

Section: Origin Of Fecal Concanavalin A-binding Glycoprotein In Ratsmentioning

confidence: 99%