Behavior and blood profile in Friesian-Holstein dairy cows in the special region of Yogyakarta, Indonesia

Abstract

The background of this study was blood profile and cows' behavior that could be used as a tool to diagnose reproductive status in the dairy cows. This research was aimed to determine biochemical blood profile, progesterone level, and behaviors of Friesian Holstein crossbreed dairy cow in the tropical climates. Fifteen Friesian-Holstein crossbreeds cows, in early lactation, reared by local farmers were divided into two barn types: individual and groups of cows. Blood samples were collected from the jugular vein using a 22G needle on days 0, 14, and 28. For the determination of blood profile, blood was collected into Vacuntainer® blood tubes containing ethylenediaminetetraacetic acid (EDTA) to arrest glycolysis. The blood was transported to the laboratory with a coolbox. The concentration of progesterone in plasma was assayed by enzyme-linked immunosorbent assay (ELISA) using a commercial kit and the biochemical blood profile serum was measured by using microlab 300. The variables observed were glucose, cholesterol, urea, and non-pregnant progesterone. Data obtained was examined by T-test analysis. The result showed that the Temperature-Humidity Index were 81.3 and 79.6 respectively, there were no significant differences between two observation, in terms of total feeding time (3.06±0.48 and 2.91±0.22 hours/day), total water consumption (86.23±0.58 and 85.37±0.88 l/day), total standing (12.70±0.21 and 12.83±0.34 hours/day), total laying (11.30±0.71 and 11.16±0.62 hours/day). The blood profile and progesterone levels were lower than the normal range in dairy cows. Also, the result indicated that blood parameters were not significantly different (P > 0.05) between individual pen and group. The result indicated that the progesterone level was not significant in two models of management dairy cows. The conclusion of the research is the climate can affect behavior and the blood parameters. Blood profiles below normal range affect fertility in Friesian-Holstein crossbreed dairy cows.

Effect of PENN-DIABEX, a novel polyherbal formulation, in high fat diet streptozotocin-induced diabetic rats

“…Additionally, renal function tests (RFTs) were conducted to measure urea and creatinine levels, while liver function tests (LFTs) were performed to determine levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). These measurements were obtained using standard protocols provided by EBRA biochemicals kits and analyzed using the Micro Lab 300 semi-automated biochemistry analyzer ( Hudaya et al, 2020 ). The level of hemoglobin (Hb) and glycosylated hemoglobin (HbA1C) was estimated by using HbA1c EZ 2.0 m.…”