Bedeutung der Gewebslagerzeit für den Erfolg nach kornealer Transplantation

Abstract

We found significantly better results in patients receiving tissues stored for an average time of 9.8 days than in those receiving corneas preserved for 1.9 days. The results are thought to be based on the loss of HLA-DR-positive cells during prolonged organ culture preservation.

Risk factors for corneal allograft rejection: intermediate results of a prospective normal-risk keratoplasty study

“…A similar observation was made by Ardjomand et al, who found a significantly better success rate in those patients who received grafts stored for more than 5 days than in patients who received grafts stored for less than 6 days [1]. One possible explanation for this phenomenon is the observation that storage of corneas leads to loss of corneal Langerhans cells [2,22], even though -in an experimental mouse model -storage of corneas in a tissue culture did not cause a loss of the ability to sensitize multiple minor H-disparate hosts [11].…”

Preservation of canine and feline corneoscleral tissue in Optisol® GS

“…Based on results of previous studies, it can be assumed that in dogs and cats, the rejection rate of stored tissue is lower than that of fresh tissue. This assumption is based on the results of Ardjomand et al, 35 who reported that the rejection rate of transplanted human corneal tissue stored for an average of 9.8 days was lower than that of corneas stored for an average of 1.9 days. These authors postulate that during storage, human cornea loses tissue antigen, and they recommend that corneal tissue be stored for 10-14 days before transplantation.…”

Immunologie der Keratoplastik: Macht HLA-Matching bei lamellären Verfahren Sinn?

“…[7]. Die Langerhans-Zellen des Transplantats wandern während der Organkultur aus dem Gewebeverband in das Kulturmedium, sodass die Dichte an Langerhans-Zellen des Transplantats mit steigender Kulturdauer absinkt [8]. Dies passt zu einer Beobachtung, dass tatsächlich ein Zusammenhang der Lagerdauer des Transplantats und dem postoperativen Abstoßungsrisiko nach perforierender Keratoplastik besteht [9].…”