Bean Seed Globulin mRNA: Translation, Characterization, and its use as a Probe Towards Genetic Engineering of Crop Plants

Hall, Timothy C.; Sun, Samuel M.; Buchbinder, Barry U.; Pyne, John W.; Bliss, F. A.; Kemp, John D.

doi:10.1007/978-1-4613-3051-6_22

Cited by 28 publications

(6 citation statements)

References 25 publications

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“…The rapidity of this autoregulation should help facilitate investigations of the sequence of events between light-signal perception and altered gene expression by defining a relatively short period over which the relevant events need be sought. In addition, because phytochrome mRNA is a lowabundance species, it may be considered more representative of the majority of structural genes expressed in eukaryotic cells than the relatively high-abundance mRNAs that have been most intensively studied in plants to date (32)(33)(34)(39)(40)(41). Thus, information gained from the phytochrome system may contribute to the general question of the regulation of gene expression.…”

Section: Discussionmentioning

confidence: 99%

Autoregulatory control of translatable phytochrome mRNA levels

Colbert

Hershey

Quail

1983

Proc. Natl. Acad. Sci. U.S.A.

135

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Translatable phytochrome mRNA represents ""5 X 10-3% of the total poly(A)-RNA present in etiolatedAvena seedlings, as determined by incorporation of radioactivity into the immunoprecipitable apoprotein in a cell-free translation system. Irradiation of such seedlings with 5-s red light induces a decline in this mRNA that is detectable within 15-30 min, shows a 50% reduction within 50-60 min, and results in a >95% reduction within 2 hr. The effect of the red light pulse is reversed by an immediately subsequent far-red pulse to the level of the far-red-light control, indicating that phytochrome exerts autoregulatory control over its own translatable mRNA level. This result necessitates -revision of existing concepts of how phytochrome concentrations are modulated in vivo. Red-light dose-response curves show that the response is sensitive to very low light levels. Conversion of <1% of the total cellular phytochrome to the biologically active far-red-absorbing form is sufficient to induce 60% of the maximal response, and 20% far-red-absorbing form saturates the response. The observed change in translatable phytochrome mRNA level is one of the most rapid phytochrome-induced.alterations in any cellular mRNA yet recorded. Thus, autoregulation of phytochrome mRNA provides an attractive opportunity to examine the early sequence of events in phytochrome control of gene expression.

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Section: Discussionmentioning

confidence: 99%

Autoregulatory control of translatable phytochrome mRNA levels

Colbert

Hershey

Quail

1983

Proc. Natl. Acad. Sci. U.S.A.

135

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“…Some of the phaseolin variation can be explained in terms of mRNAs of different molecular weights Hall et al 1978) which reflect the phaseolin polypeptide differences of the three different phaseolin banding types . Further phaseolin variation is due to differential glycosylation of the polypeptides (Hall et al 1978;Hall et al 1980) and this may reflect point mutations which have changed the amino acid sequence of the proteins to cause the loss or gain of post-translational glycosylation sites.…”

Section: Linkage Relationships Between Genes Controlling Seed Proteinsmentioning

confidence: 99%

Linkage relationships between genes controlling seed proteins in French bean

Brown

Bliss

Hall

1981

Theoret. Appl. Genetics

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The inheritance of phaseolin and globulin-2 (G2)/albumin polypeptides was investigated in crosses involving varieties which exhibited the three electrophoretic banding patterns of phaseolin found in French bean. 'Total' seed protein extracts of single seeds of the F1 and F2 generations from the crosses: 'Sanilac' × 'Contender', 'BBL 240' × 'Contender', and 'Sanilac' × 'BBL 240' were analyzed by two-dimensional electrophoresis. Segregation of the genes controlling phaseolin and G2/albumin polypeptides, and those controlling a further five groups of seed proteins (A, B, D, E, and F) were observed. No recombinant electrophoretic phenotypes were seen for phaseolin or G2/albumin polypeptides suggesting that the genes controlling each of these groups of polypeptides are closely linked and segregate like single Mendelian genes. The phaseolin genes and G2/albumin genes were not linked to each other. The group of genes controlling phaseolin polypeptides were linked to those controlling group B proteins, and those controlling G2/albumin polypeptides were linked to those controlling group F proteins.

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“…Some of the events postulated in Figure 8 are not without analogies in other higher plants. Differences in the degree of glycosylation among members of storage protein sets that appear to represent a multigene family because of immunological crossreactivity, and that result in large differences in apparent molecular weights, have been found in Phaseolus vulgaris (13) Figure 6. S 1 1 .…”

Section: Andmentioning

confidence: 99%

Developmental Biochemistry of Cottonseed Embryogenesis and Germination

Dure¹,

Galau²

1981

Plant Physiol.

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The synthesis of the principal cottonseed storage proteins during embryogenesis has been foUlowed by analyses of stained protein gels and of fluorographs of protein synthesized in vivo and from purified RNA in vitro in the wheat germ system. The kinetics of in vivo labeling as well as immunochemical cross-reactivity indicate that the 52-and 48-kiLodalton mature storage protein sets are derived from 70-and 67-kilodalton precursor protein sets that are abundant proteins in embryonic cotyledons and disappear in late embryogenesis. Identification of the initial translation products of the storage protein mRNA has not been clearly established although products of apparent molecular weights of 69,000 and 60,000 are the likely storage protein precursors.Storage protein synthesis falls off markedly in late embryogenesis simultaneously with the loss of a superabundant class of mRNAs (shown by cDNA:RNA reassociation) that are presumed to be those for the storage proteins. The synthesis of these proteins ceases abruptly when immature embryos are removed from the boll and allowed to germinate precociously or when this precocious germination is prevented by incubation in abscisic acid. Thus, abscisic acid is not implicated in the expression of the storage protein genes.A scheme involving co-translational processing into vesicles, glycosylation, and slow in situ cleavage to produce the mature storage proteins is proposed.We have attempted to follow during embryogenesis the synthesis of the principal storage proteins of cotton cotyledons which were identified and partially characterized in the preceding paper (8). The accumulation of these proteins (and their precursors) was followed by the 2D2 electrophoresis of proteins extracted at intervals during embryogenesis. To follow their synthesis, the incorporation of radioactive amino acids into them in intact cotyledons was assessed by the fluorography of 2D protein gels (in vivo synthesis) and estimates of the levels of their mRNAs were made by measuring the synthesis of their initial translation forms with mRNA isolated at stages in embryogenesis utilizing the wheat germ in vitro protein-synthesizing system.The measurements reported here were made with cotyledons taken from embryos that were 50 and 100 mg in wet weight and from cotyledons from the mature seed. with a solution containing the '4C-labeled algal hydrolysate (5 ,iCi ml-'), gramacidin D (10 Ag ml-') and, where indicated, AbA (5 EAM). After the incubation period, the embryos were thoroughly rinsed in distilled H20, axes removed and discarded, and the cotyledons extracted for protein as given above. In the pulse-chase experiment, the embryos were removed from the filter paper 187 www.plantphysiol.org on May 9, 2018 -Published by Downloaded from

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Bean Seed Globulin mRNA: Translation, Characterization, and its use as a Probe Towards Genetic Engineering of Crop Plants

Cited by 28 publications

References 25 publications

Autoregulatory control of translatable phytochrome mRNA levels

Autoregulatory control of translatable phytochrome mRNA levels

Linkage relationships between genes controlling seed proteins in French bean

Developmental Biochemistry of Cottonseed Embryogenesis and Germination

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