Linkage relationships between genes controlling seed proteins in French bean

Abstract

The inheritance of phaseolin and globulin-2 (G2)/albumin polypeptides was investigated in crosses involving varieties which exhibited the three electrophoretic banding patterns of phaseolin found in French bean. 'Total' seed protein extracts of single seeds of the F1 and F2 generations from the crosses: 'Sanilac' × 'Contender', 'BBL 240' × 'Contender', and 'Sanilac' × 'BBL 240' were analyzed by two-dimensional electrophoresis. Segregation of the genes controlling phaseolin and G2/albumin polypeptides, and those controlling a further five groups of seed proteins (A, B, D, E, and F) were observed. No recombinant electrophoretic phenotypes were seen for phaseolin or G2/albumin polypeptides suggesting that the genes controlling each of these groups of polypeptides are closely linked and segregate like single Mendelian genes. The phaseolin genes and G2/albumin genes were not linked to each other. The group of genes controlling phaseolin polypeptides were linked to those controlling group B proteins, and those controlling G2/albumin polypeptides were linked to those controlling group F proteins.

Chromosomal Location of Lectin Genes Indicates They Are Not the Basis of Rhizobium Strain Specificity Mutations Identified in Pea (Pisum sativum L.)

“…However, genetic linkage of the multigene family of the Phaseolus seed lectins has been reported (Brown et al 1981;Hoffman and Donaldson 1985). In addition, proteins such as ribulose bisphosphate carboxylase, actin, and leghemoglobin are coded by small families of genes, and usually at least a portion of the genes within the family is tightly linked.…”

Phaseolin: Structure and Evolution

“…The banding patterns obtained by single and two-dimensional gel electrophoretic analyses, indicate that Contender is a composite of Tendergreen and Sanilac types, showing a good agreement between the number of phaseolin protomers and structural genes. Genetic analysis revealed a tight linkage for the entire phaseolin gene family [17]. Heteroduplex analysis of the phaseolin genomic clones [36] revealed that whereas the phaseolin DNA coding sequences were similar, considerable divergence occurred close to their 5' and 3' termini [37].…”

“…The ability to obtain large quantities of purified phaseolin facilitated electrophoretic studies using one dimensional SDS gels [12] and characterization by equilibrium sedimentation [13]. These initial forays paved the way for biophysical characterization [14][15][16][17] and the subsequent crystallographic structural analysis [18][19][20][21].…”

Determination of Phaseolin Types in Common Bean (Phaseolus vulgaris) Varieties from Turkey

“…Common bean contains higher levels of protein (>20%) compared to cereals such as rice and wheat (<15%) (Kaplan, 1965;Brown et al, 1981a;Chandrakanth and Hall, 2008). Phaseolin, a protein mainly stored in seeds, makes up the majority of total seed protein.…”

“…Type "B" phaseolin is present only in wild and cultivated varieties of Colombia. Such variety in phaseolin types is thought to have resulted from random outcrosses in nature, contaminations from cultivation areas and multiple cultivation efforts (Brown et al, 1981a;Koenig et al, 1990;Kami et al, 1995).…”