Bead‐based microarray analysis of microRNA expression in hepatocellular carcinoma: miR‐338 is downregulated

Abstract

Aim: Recent studies have underlined causative links between microRNA (miRNA) deregulation and cancer development. However, the relevance of abnormally expressed miRNA to tumor biology has not been well understood in hepatocellular carcinoma (HCC).Methods: A bead-based miRNA expression profiling method was performed on 20 pairs of surgically removed HCC and adjacent non-tumorous tissue (NT). Special miR-338 downregulations and miR-338 associated with clinical characteristics was validated in an extended samples set of 36 paired HCC and adjacent non-tumorous liver tissues by real-time reverse transcription polymerase chain reaction (RT-PCR) analysis.Results: Out of our bead-based microarray data, 12 upregulated and 19 downregulated miRNA were found to be associated with HCC. Further characterization of miRNA-338, in which 20 pairs of the samples were clustered clearly into two groups according to expression of miR-338, revealed that the level of miR-338 expression can be associated with clinical aggressiveness, such as, tumor size, tumor-node-metastasis stage, vascular invasion and intrahepatic metastasis. These results were validated by real-time RT-PCR analysis. Conclusion:Our study suggests that miRNA expression could have relevance to the clinical behavior of HCC and that the bead-based miRNA expression profiling method might be a suitable system to assay miRNA expression in large-scale diagnostic trails.

Clinicopathological and prognostic significance of microRNA-107 and its relationship to DICER1 mRNA expression in gastric cancer

“…Relationship between clinicopathological features and miR-107 expression in gastric cancer tissues. Many studies have demonstrated overexpression of miR-107 in gastric, esophageal, colon, hepatocellular and pancreatic cancers, suggesting that this miRNA has some positive role in the carcinogenesis of these cancers (10)(11)(12)(13)(14)(15)(16). It has been reported that miR-107 is an oncogenic miRNA that regulates tumor invasion and metastasis in gastric cancer (13).…”

“…Recent evidence indicates that various miRNAs can function as oncogenes or tumor-suppressor genes and play a central role in carcinogenesis (5). Accumulating evidence shows that miR-107 is one of the oncogenic RNAs, and overexpression of these RNAs has been reported in several types of human malignant solid tumors, including gastric, esophageal, pancreatic and colorectal cancer (10)(11)(12)(13)(14)(15)(16). Moreover, miR-107 may contribute to the regulation of the cell cycle in non-small cell lung cancer cell lines (17).…”

miR-338-3p Suppresses Gastric Cancer Progression through a PTEN-AKT Axis by Targeting P-REX2a

“…The earlier studies proved that miR-338-3p is downregulated in hepatocellular carcinoma (12) and malignant melanoma (13). In addition, miR-338-3p could suppress progression of cancer cells by targeting smoothened in both liver cancer (14) and colorectal carcinoma (15).…”

Construction of lentivirus-based inhibitor of hsa-microRNA-338-3p with specific secondary structure

“…Tsuchiya et al [16] have reported that miR-338-3p contributes to the formation of epithelial basolateral polarity by facilitating the translocalization of β1-integrin to the basolateral membrane, which highlights a potentially important role for miR-338-3p in the process of epithelial cell differentiation. Huang et al [17] demonstrated that a decrease of miR-338-3p expression in hepatocellular carcinoma (HCC), which is another type of epithelial cell-derived cancer, was significantly associated with TNM stage, vascular invasion, intrahepatic metastasis, tumor size and tumor grade. In our previous study, we demonstrated that miR-338-3p expression was down-regulated in both CRC samples and CRC-derived cell lines.…”