Clinicopathological and prognostic significance of microRNA-107 and its relationship to DICER1 mRNA expression in gastric cancer

Inoue, Taisuke; Iinuma, Hisae; Ogawa, Etsushi; Inaba, Tomoki; Fukushima, Ryoji

doi:10.3892/or.2012.1709

Cited by 47 publications

(44 citation statements)

References 25 publications

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“…23, 24 In addition, higher expression of miRNAs that target Dicer might be another critical mechanism, for example, miR103/107 were reported to target and downregulate Dicer expression and be associated with poor survival in breast cancer 15 and gastric cancer. 25 let-7 expression was inversely correlated with Dicer expression and constituted a negative feedback loop controlling Dicer expression in a panel of cancer cell lines. 16 Interestingly, miR-107 can directly interact with let-7 and reduce its expression, 26 suggesting a complex relationship between miR-107, let-7 and Dicer.…”

Section: Discussionmentioning

confidence: 99%

Prognostic significance of low DICER expression regulated by miR-130a in cervical cancer

Wang

Zhang

et al. 2014

Cell Death Dis

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Dicer is crucial for the maturation of microRNAs (miRNAs) and its dysregulation may contribute to tumor initiation and progression. The study explored the clinical implications of Dicer and its post-transcriptional regulation by microRNAs in cervical cancer. qRT-PCR and immunohistochemistry investigated Dicer mRNA and protein levels in cervical cancer tissues. The relationship between Dicer expression and survival was analyzed. MiRNA target prediction identified miRNAs that might target Dicer. Luciferase reporter and gain- or loss-of-function assays were performed. The results showed that 36.7% of cervical cancer cases showed low expression of Dicer mRNA and 63.3% cases showed high expression. At the protein level, 51% cases showed negative expression and 49% cases showed positive expression. Dicer mRNA and protein expressions were significantly associated with distant metastasis and recurrence in cervical cancer (P=0.002 and P=0.012, respectively). Multivariate Cox analysis indicated that low Dicer expression (P=0.016) and tumor stage (P=0.047) were independent predictors. Among the miRNAs predicted to target Dicer, 10 were detected by RT-PCR; their expressions were significantly higher in cervical cancers with lower Dicer expression than in those with higher Dicer expression and were negatively correlated with Dicer expression level (P<0.05). In vitro experiments demonstrated that miR-130a directly targeted Dicer mRNA to enhance migration and invasion in SiHa cells. Finally, survival analysis indicated that higher expression of miR-130a was significantly associated with poor disease-free survival. Taken together, Dicer expression regulated by miR-130a is an important potential prognostic factor in cervical cancer.

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Section: Discussionmentioning

confidence: 99%

Prognostic significance of low DICER expression regulated by miR-130a in cervical cancer

Wang

Zhang

et al. 2014

Cell Death Dis

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“…In the Cox multivariate analysis, it was shown that miR-107 expression in GC tissues was an independent prognostic factor for OS and DFS. Their results indicate that miR-107 may be useful as an effective biomarker for prediction of a poor prognosis in GC patients [13]. However, the detailed mechanisms of miR-107 were fewly investigated in GC.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, Inoue et al found that the mean expression level of miR-107 was significantly higher in the GC tissues compared to that of normal tissues. In the comparison of clinicopathological factors, miR-107 expression showed significant association with depth of tumor invasion, lymph node metastasis and tumor stage [13]. However, the detailed mechanisms of miR-107 were fewly investigated in GC.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-107 promotes proliferation of gastric cancer cells by targeting cyclin dependent kinase 8

Song

et al. 2014

Diagn Pathol

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BackgroundThe biological processes and molecular mechanisms underlying miR-107 remain unclear in gastric cancer(GC). In this study, we aimed to investigate the expression, biological functions and mechanisms of miR-107 in GC.MethodsQuantitative real-time RT-PCR was used to test miR-107 expression. MTT and colony formation assays were conducted to explore the potential function of miR-107 in human GC cell line SGC7901. The target gene was determined by bioinformatic algorithms, dual luciferase reporter assay, RT-PCR and Western blot.ResultsExpression of miR-107 was significantly elevated in GC cell line than that in gastric epithelial cell line(p = 0.012). We found that miR-107 inhibitor transfection significantly decreased the proliferation of GC cell line, and clone formation rate of miR-107 inhibitor transfected group was significantly lower than that of control group. Luciferase assays using a reporter carrying a putative miR-107 target site in the 3′untranslated region (3′-UTR) of cyclin dependent kinase 8 (CDK8) revealed that miR-107 directly targets CDK8. The expression level of CDK8 mRNA and protein in miR-107 inhibitor transfected GC cell line was significantly decreased compared with control group.ConclusionOur findings indicate that miR-107 is upregulated in GC and affects the proliferation of GC cells, partially through the regulation of CDK8.Virtual SlidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_164

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“…Finally, a circRNA-microRNA-gene regulatory network was constructed consisting of 22 circRNAs, 17 microRNAs and 130 PCGs (Fig 5, S3 Table). Among these microRNAs, several microRNAs were found related to cancers, such as has-miR-34a-5p has been reported related to several types of cancers, for example, colorectal cancer [36]; has-miR-107 exhibit aberrant expression in a variety of cancers, including hepatocellular [37], gastric cancer [38], and Glioma [39] etc. The GO analysis suggested that these PCGs mainly involved in metabolic and regulation-related process (Fig 6A).…”

Section: Resultsmentioning

confidence: 99%

Computational identifying and characterizing circular RNAs and their associated genes in hepatocellular carcinoma

Dong

Huang

et al. 2017

PLoS ONE

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Hepatocellular carcinoma (HCC) is currently still a major factor leading to death, lacking of reliable biomarkers. Therefore, deep understanding the pathogenesis for HCC is of great importance. The emergence of circular RNA (circRNA) provides a new way to study the pathogenesis of human disease. Here, we employed the prediction tool to identify circRNAs based on RNA-seq data. Then, to investigate the biological function of the circRNA, the candidate circRNAs were associated with the protein-coding genes (PCGs) by GREAT. We found significant candidate circRNAs expression alterations between normal and tumor samples. Additionally, the PCGs associated with these candidate circRNAs were also found have discriminative expression patterns between normal and tumor samples. The enrichment analysis illustrated that these PCGs were predominantly enriched for liver/cardiovascular-related diseases such as atherosclerosis, myocardial ischemia and coronary heart disease, and participated in various metabolic processes. Together, a further network analysis indicated that these PCGs play important roles in the regulatory and the PPI network. Finally, we built a classification model to distinguish normal and tumor samples by using candidate circRNAs and their associated genes, respectively. Both of them obtained satisfactory results (~ 0.99 of AUC for circRNA and PCG). Our findings suggested that the circRNA could be a critical factor in HCC, providing a useful resource to explore the pathogenesis of HCC.

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Clinicopathological and prognostic significance of microRNA-107 and its relationship to DICER1 mRNA expression in gastric cancer

Cited by 47 publications

References 25 publications

Prognostic significance of low DICER expression regulated by miR-130a in cervical cancer

Prognostic significance of low DICER expression regulated by miR-130a in cervical cancer

MicroRNA-107 promotes proliferation of gastric cancer cells by targeting cyclin dependent kinase 8

Computational identifying and characterizing circular RNAs and their associated genes in hepatocellular carcinoma

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