BdorOR88a Modulates the Responsiveness to Methyl Eugenol in Mature Males of Bactrocera dorsalis (Hendel)

Liu, Huan; Chen, Zheng-Shi; Zhang, Dongju; Lu, Yongyue

doi:10.3389/fphys.2018.00987

Cited by 41 publications

(46 citation statements)

References 120 publications

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“…In B. dorsalis , it is well known that methyl eugenol (ME) is strongly attractive to males [ 14 ]. Based on the high potency and sex pheromone-like behavior of ME, trapping systems have been developed to manage B. dorsalis and as well as additional molecular mechanistic analysis of B. dorsalis- ME interactions [ 15 , 16 ], but primarily with male flies. However, the oviposition by females is the main mechanism of fruit damage by insects.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic analysis reveals female-biased olfactory genes potentially involved in plant volatile-mediated oviposition behavior of Bactrocera dorsalis

Tang

Chen

et al. 2021

BMC Genomics

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Background Olfactory systems take on important tasks to distinguish salient information from a complex olfactory environment, such as locating hosts, mating, aggression, selecting oviposition sites, and avoiding predators. The olfactory system of an adult insect consists of two pairs of main olfactory appendages on the head, the antennae, and the palps, which are covered with sensilla. Benzothiazole and 1-octen-3-ol could elicit oviposition behavior in gravid B. dorsalis are regarded as oviposition stimulants. However, the mechanism for how B. dorsalis percepts benzothiazole and 1-octen-3-ol still remains unknown. Results We conducted a comparative analysis of the antennal transcriptomes in different genders of B. dorsalis using Illumina RNA sequencing (RNA-seq). We identified a total of 1571 differentially expressed genes (DEGs) among the two sexes, including 450 female-biased genes and 1121 male-biased genes. Among these DEGs, we screened out 24 olfaction-related genes and validated them by qRT-PCR. The expression patterns of these genes in different body parts were further determined. In addition, we detected the expression profiles of the screened female-biased chemosensory genes in virgin and mated female flies. Furthermore, the oviposition stimulants-induced expression profilings were used to identify chemosensory genes potentially responsible for benzothiazole and 1-octen-3-ol perception in this fly. Conclusions The results from this study provided fundamental data of chemosensory DEGs in the B. dorsalis antenna. The odorant exposure assays we employed lay a solid foundation for the further research regarding the molecular mechanism of benzothiazole and 1-octen-3-ol mediated oviposition behavior in B. dorsalis.

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Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic analysis reveals female-biased olfactory genes potentially involved in plant volatile-mediated oviposition behavior of Bactrocera dorsalis

Tang

Chen

et al. 2021

BMC Genomics

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“…We believe that these findings support our suggestion that the maxillary palps be an additional focal point of olfactory research in Tephritid fruit flies. This can hopefully be combined with extensive work conducted recently on the transcriptomes of the oriental fruit fly and other Tephritid species focusing on identifying genes and proteins in these species involved in chemoreception ( Zheng et al 2013 ; Wu et al 2015 ; Elfekih et al 2016 ; Liu et al 2016 ; 2017 , 2018 ; Miyazaki et al 2018 ) as part of the search for novel ways to control these highly destructive and invasive pests.…”

Section: Discussionmentioning

confidence: 99%

“…Extending the seminal work of Metcalf and coworkers done over four decades ago describing how B. dorsalis males respond to ME ( Metcalf et al 1975 ), more recent reviews of the literature on B. dorsalis olfaction, especially that pertaining to ME, have focused almost entirely on the role of the antennae ( Zheng et al 2013 ; Wu et al 2016 ; Liu et al 2017 , 2018 ). While it has been established that the ability of these male flies to be attracted to lures such as ME depends on the presence and activation of olfactory receptor neurons in the sensilla of the antenna ( Zheng et al 2012 ; Liu et al 2017 , 2018 ), it is also known that olfactory sensilla also exist in the maxillary palp ( Zhang et al 2011 ). This includes the type of sensilla basiconica earlier described as thin-walled multiporous pitted sensilla ( Dickens et al 1988 ) that are also present in the antenna ( Lee et al 1994 ).…”

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confidence: 99%

Involvement of the Antennal and Maxillary Palp Structures in Detection and Response to Methyl Eugenol by Male Bactrocera dorsalis (Diptera: Tephritidae)

Chieng

Hee

Wee

2018

Journal of Insect Science

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The oriental fruit fly, Bactrocera dorsalis (Handel) is one of the most destructive pests of fruits. The discovery of methyl eugenol (ME) as a potent male attractant for this species has led to its successful use in area-wide fruit fly control programs such as male annihilation. While the antenna is recognized as primarily responsible for male flies’ detection of attractants such as ME, little is known of the involvement of the maxillary palp. Using behavioral assays involving males with intact and ablated antennae and maxillary palp structures, we seek to ascertain the relative involvement of the maxillary palp in the ability of the male fly to detect ME. In cage bioassays (distance of ≤40 cm from the source), >97% of unmodified males will normally show a response to ME. Here, we showed that 17.6% of males with their antennae ablated were still attracted to ME versus 75.0% of males with their palps ablated. However, none of the antennae-ablated males were able to detect ME over a distance of >100 cm. Furthermore, wind tunnel bioassays showed that maxillary palp-ablated males took a significantly longer time compared to unablated males to successfully detect and eventually feed on ME. These results suggest that although the antennae are necessary for detection of ME over longer distances, at shorter distances, both antennae and maxillary palps are also involved in detecting ME. Hence, those palps may play a larger role than previously recognized in maneuvering males toward lure sources over shorter ranges.

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“…ORs are considered to be chemosensory receptors for male attractants in Dacini fruit flies. Indeed, OR88a has been characterized as a candidate ME receptor in B. dorsalis by heterologous expression using Xenopus oocytes, and behavioral bioassays of male flies treated with RNAi-mediated OR88a knockdown [135]. The involvement of the OR system in the chemoreception of ME has also been shown by RNAi-mediated ORCO knockdown in B. dorsalis [134].…”

Section: Future Research 61 Characterization Of Chemosensory Receptmentioning

confidence: 99%

“…Furthermore, RNAi-mediated knockdown of ORCO reduced the attractiveness of ME in the treated males. Most importantly, a recent study characterized OR88a as the ME receptor [ 135 ]. This study showed that the transcriptional level of OR88a was enhanced by exposure to ME, as seen for ORCO .…”

Section: Major Molecular Components In Insect Chemoreceptionmentioning

confidence: 99%

Recent Advancements in Studies on Chemosensory Mechanisms Underlying Detection of Semiochemicals in Dacini Fruit Flies of Economic Importance (Diptera: Tephritidae)

Ono

Hee

Jiang

2021

Insects

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Dacini fruit flies mainly contain two genera, Bactrocera and Zeugodacus, and include many important pests of fruits and vegetables. Their life cycle is affected by various environmental cues. Among them, multiple characteristic semiochemicals have remarkable effects on their reproductive and host-finding behaviors. Notably, floral fragrances released from so-called fruit fly orchids strongly attract males of several Dacini fruit fly species. Focusing on the strong attraction of male flies to particular chemicals, natural and synthetic lures have been used for pest management. Thus, the perception of semiochemicals is important to understand environmental adaptation in Dacini fruit flies. Since next-generation sequencers are available, a large number of chemosensory-related genes have been identified in Dacini fruit flies, as well as other insects. Furthermore, recent studies have succeeded in the functional analyses of olfactory receptors in response to semiochemicals. Thus, characterization of molecular components required for chemoreception is under way. However, the mechanisms underlying chemoreception remain largely unknown. This paper reviews recent findings on peripheral mechanisms in the perception of odors in Dacini fruit flies, describing related studies in other dipteran species, mainly the model insect Drosophilamelanogaster. Based on the review, important themes for future research have also been discussed.

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BdorOR88a Modulates the Responsiveness to Methyl Eugenol in Mature Males of Bactrocera dorsalis (Hendel)

Cited by 41 publications

References 120 publications

Comparative transcriptomic analysis reveals female-biased olfactory genes potentially involved in plant volatile-mediated oviposition behavior of Bactrocera dorsalis

Comparative transcriptomic analysis reveals female-biased olfactory genes potentially involved in plant volatile-mediated oviposition behavior of Bactrocera dorsalis

Involvement of the Antennal and Maxillary Palp Structures in Detection and Response to Methyl Eugenol by Male Bactrocera dorsalis (Diptera: Tephritidae)

Recent Advancements in Studies on Chemosensory Mechanisms Underlying Detection of Semiochemicals in Dacini Fruit Flies of Economic Importance (Diptera: Tephritidae)

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