In insects, control of body size is intimately linked to nutritional quality as well as environmental and genetic cues that regulate the timing of developmental transitions. Prothoracicotropic hormone (PTTH) has been proposed to play an essential role in regulating the production and/or release of ecdysone, a steroid hormone that stimulates molting and metamorphosis. In this report, we examine the consequences on Drosophila development of ablating the PTTH-producing neurons. Surprisingly, PTTH production is not essential for molting or metamorphosis. Instead, loss of PTTH results in delayed larval development and eclosion of larger flies with more cells. Prolonged feeding, without changing the rate of growth, causes the overgrowth and is a consequence of low ecdysteroid titers. These results indicate that final body size in insects is determined by a balance between growth-rate regulators such as insulin and developmental timing cues such as PTTH that set the duration of the feeding interval.

Abstract. In this paper we study compact Sasaki manifolds in view of transverse Kähler geometry and extend some results in Kähler geometry to Sasaki manifolds. In particular we define integral invariants which obstruct the existence of transverse Kähler metric with harmonic Chern forms. The integral invariant f 1 for the first Chern class case becomes an obstruction to the existence of transverse Kähler metric of constant scalar curvature. We prove the existence of transverse Kähler-Ricci solitons (or Sasaki-Ricci soliton) on compact toric Sasaki manifolds whose basic first Chern form of the normal bundle of the Reeb foliation is positive and the first Chern class of the contact bundle is trivial. We will further show that if S is a compact toric Sasaki manifold with the above assumption then by deforming the Reeb field we get a Sasaki-Einstein structure on S. As an application we obtain irregular toric Sasaki-Einstein metrics on the unit circle bundles of the powers of the canonical bundle of the two-point blow-up of the complex projective plane.

Ecdysteroids regulate many key developmental events in arthropods including molting and metamorphosis. Recently, members of the Drosophila Halloween group of genes, that are required for embryonic viability and cuticle deposition, have been shown to code for several cytochrome P450 enzymes that catalyze the terminal hydroxylation steps in the conversion of cholesterol to the molting hormone 20-hydroxyecdysone. These P450s are conserved in other insects and each is thought to function throughout development as the sole mediator of a particular biosynthetic step since, where analyzed, each is expressed at all stages of development and shows no closely related homolog in their respective genomes. In contrast, we show here that several dipteran genomes encode two novel, highly related, microsomal P450 enzymes, Cyp307A1 and Cyp307A2, that likely participate as stage-specific components of the ecdysone biosynthetic machinery. This hypothesis comes from the observation that Cyp307A1 is encoded by the Halloween gene spook (spo), but unlike other Halloween class genes, Dmspo is not expressed during the larval stages. In contrast, Cyp307a2, dubbed spookier (spok), is expressed primarily during larval stages within the prothoracic gland cells of the ring gland. RNAi mediated reduction in the expression of this heterochromatin localized gene leads to arrest at the first instar stage which can be rescued by feeding the larva 20E, E or ketodiol but not 7dC. In addition, spok expression is eliminated in larvae carrying mutations in molting defective (mld), a gene encoding a nuclear zinc finger protein that is required for production of ecdysone during Drosophila larval development. Intriguingly, mld is not present in the Bombyx mori genome, and we have identified only one spook homolog in both Bombyx and Manduca that is expressed in both embryos and larva. These studies suggest an evolutionary split between Diptera and Lepidoptera in how the ecdysone biosynthetic pathway is regulated during development.

Bactrocera papayae Drew & Hancock, Bactrocera philippinensis Drew & Hancock, Bactrocera carambolae Drew & Hancock, and Bactrocera invadens Drew, Tsuruta & White are four horticultural pest tephritid fruit fly species that are highly similar, morphologically and genetically, to the destructive pest, the Oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). This similarity has rendered the discovery of reliable diagnostic characters problematic, which, in view of the economic importance of these taxa and the international trade implications, has resulted in ongoing difficulties for many areas of plant protection and food security. Consequently, a major international collaborative and integrated multidisciplinary research effort was initiated in 2009 to build upon existing literature with the specific aim of resolving biological species limits among B. papayae, B. philippinensis, B. carambolae, B. invadens and B. dorsalis to overcome constraints to pest management and international trade. Bactrocera philippinensis has recently been synonymized with B. papayae as a result of this initiative and this review corroborates that finding; however, the other names remain in use. While consistent characters have been found to reliably distinguish B. carambolae from B. dorsalis, B. invadens and B. papayae, no such characters have been found to differentiate the latter three putative species. We conclude that B. carambolae is a valid species and that the remaining taxa, B. dorsalis, B. invadens and B. papayae, represent the same species. Thus, we consider B. dorsalis (Hendel) as the senior synonym of B. papayae Drew and Hancock syn.n. and B. invadens Drew, Tsuruta & White syn.n. A redescription of B. dorsalis is provided. Given the agricultural importance of B. dorsalis, this taxonomic decision will have significant global plant biosecurity implications, affecting pest management, quarantine, international trade, postharvest treatment and basic research. Throughout the paper, we emphasize the value of independent and multidisciplinary tools in delimiting species, particularly in complicated cases involving morphologically cryptic taxa. Bactrocera (Bactrocera) dorsalis (Hendel)

Abstract. In [11] it was proved that, given a compact toric Sasaki manifold with positive basic first Chern class and trivial first Chern class of the contact bundle, one can find a deformed Sasaki structure on which a SasakiEinstein metric exists. In the present paper we first prove the uniqueness of such Einstein metrics on compact toric Sasaki manifolds modulo the action of the identity component of the automorphism group for the transverse holomorphic structure, and secondly remark that the result of [11] implies the existence of compatible Einstein metrics on all compact Sasaki manifolds obtained from the toric diagrams with any height, or equivalently on all compact toric Sasaki manifolds whose cones have flat canonical bundle. We further show that there exists an infinite family of inequivalent toric Sasaki-Einstein metrics on S 5 ♯k(S 2 × S 3 ) for each positive integer k.

Males of many tephritid fruit fly species of the genus Bactrocera show a very strong affinity to methyl eugenol (ME). An attracted male compulsively ingests ME, which is then biotransformed before its metabolites are accumulated into the rectal gland. The glandular organ is known to serve as a reservoir for sex pheromone in some species. Upon ME-feeding, males of the oriental fruit fly, Bactrocera dorsalis, selectively accumulated two metabolites, 2-allyl-4,5-dimethoxyphenol (DMP) and (E)-coniferyl alcohol (E-CF), in the rectal pheromone gland. We compared the profiles of phenylpropanoid metabolites accumulated by three other species of very high economic and quarantine importance-Bactrocera invadens, Bactrocera zonata and Bactrocera correcta, with that of B. dorsalis. Males of each species were fed artificially on ME and the metabolites stored in the rectal glands were examined by means of chromatography and spectroscopy. Similar to B. dorsalis, males of laboratory-raised B. invadens accumulated DMP and E-CF, in almost equal quantities, in the rectal sac. The sum of DMP and E-CF increased gradually with time after ME consumption and reached as high as 150 lg/male 2 days post ME-feeding. Wild males of B. invadens captured in Kenya also possessed both the compounds in varying quantities. In contrast, males of B. zonata accumulated DMP and (Z)-coniferyl alcohol (Z-CF) in an approximate ratio of 1:1; whereas B. correcta is known to convert ME to (Z)-3,4-dimethoxycinnamyl alcohol (Z-DMC) and Z-CF also in an approximately 1:1 ratio. Thus, there are three types of binary combinations of rectal phenylpropanoid volatiles (i.e. DMP ? E-CF; DMP ? Z-CF; Z-CF ? Z-DMC) utilized among the four Bactrocera species. Such differences in phenylpropanoid ingredients may play a critical role in differentiating these species if encountered in the natural habitat. In this context, the two putative sibling species-B. invadens and B. dorsalis, possess the identical subset of rectal volatiles (DMP and E-CF) in a similar proportion. Furthermore, the phylogenetic analyses of the four Bactrocera species by comparing nucleotide sequences in the mitochondrial genes showed that B. invadens clearly belonged to the same clade as B. dorsalis species. Therefore, we consider the two as the same biological species, and certainly not distinct.

The Oriental fruit fly, Bactrocera dorsalis, is a highly destructive pest of various fruits. The reproductive and host-finding behaviors of this species are affected by several plant semiochemicals that are perceived through chemosensory receptors. However, the chemosensory mechanisms by which this perception occurs have not been fully elucidated. We conducted RNA sequencing analysis of the chemosensory organs of B. dorsalis to identify the genes coding for chemosensory receptors. We identified 60 olfactory receptors (ORs), 17 gustatory receptors and 23 ionotropic receptors-including their homologs and variants-from the transcriptome of male antennae and proboscises. We functionally analyzed ten ORs co-expressed with the obligatory co-receptor ORCO in Xenopus oocytes to identify their ligands. We tested 24 compounds including attractants for several Bactrocera species and volatiles from the host fruits of B. dorsalis. We found that BdorOR13a co-expressed with ORCO responded robustly to 1-octen-3-ol. BdorOR82a co-expressed with ORCO responded significantly to geranyl acetate, but responded weakly to farnesenes (a mixture of isomers) and linalyl acetate. These four compounds were subsequently subjected to behavioral bioassays. When each of the aforementioned compound was presented in combination with a sphere model as a visual cue to adult flies, 1-octen-3-ol, geranyl acetate, and farnesenes significantly enhanced landing behavior in mated females, but not in unmated females or males. These results suggest that the ORs characterized in the present study are involved in the perception of plant volatiles that affect host-finding behavior in B. dorsalis.

Human milk lysozyme is thought to be a key defense factor in protecting the gastrointestinal tract of newborns against bacterial infection. Recently, evidence was found that pepsin, under conditions relevant to the newborn stomach, cleaves chicken lysozyme (cLZ) at specific loops to generate five antimicrobial peptide motifs. This study explores the antimicrobial role of the corresponding peptides of human lysozyme (hLZ), the actual protein in breast milk. Five peptide motifs of hLZ, one helix-loop-helix (HLH), its two helices (H1 and H2), and two helix-sheet motifs, H2-β-strands 1-2 (H2-S12) or H2-β-strands 1-3 (H2-S13), were synthesized and examined for antimicrobial action. The five peptides of hLZ exhibit microbicidal activity to various degrees against several bacterial strains. The HLH peptide and its N-terminal helix (H1) were significantly the most potent bactericidal to Gram-positive and Gram-negative bacteria and the fungus Candida albicans . Outer and inner membrane permeabilization studies, as well as measurements of transmembrane electrochemical potentials, provided evidence that HLH peptide and its N-terminal helix (H1) kill bacteria by crossing the outer membrane of Gram-negative bacteria via self-promoted uptake and are able to dissipate the membrane potential-dependent respiration of Gram-positive bacteria. This finding is the first to describe that hLZ possesses multiple antimicrobial peptide motifs within its N-terminal domain, providing insight into new classes of antibiotic peptides with potential use in the treatment of infectious diseases.