Bdellovibrio lyse multiple pathogenic bacteria and protect crucian carp Carassius auratus gibelio from Aeromonas veronii infections

Yang, Hui; Cao, Qin; Zhu, Zhu; Cao, Yangyang; Ji, Tongwei; Wei, Wenzhi; Zhao, Huijing; Zhang, Yingying

doi:10.1016/j.aquaculture.2022.738741

Cited by 7 publications

(3 citation statements)

References 35 publications

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“…These phyla were both associated with neurotransmitter levels such as serotonin in the cerebral cortex and hippocampus in rats [53]. The phylum Bdellovibrionota was also positively associated with increased activity in our tadpole behavioural assays, and recent studies have described this phylum to contain natural antibiotics that positively influence gut microbial community composition through protection from pathogens [54], although to our knowledge it has yet to be associated with behavioural parameters in vertebrates. The phylum Firmicutes, one of the most abundant phyla across treatments, was negatively associated with tadpole activity.…”

Section: Discussionmentioning

confidence: 92%

Something in the water: aquatic microbial communities influence the larval amphibian gut microbiota, neurodevelopment and behaviour

Emerson,

Woodley

2024

Proc. R. Soc. B.

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Microorganisms colonize the gastrointestinal tract of animals and establish symbiotic host-associated microbial communities that influence vertebrate physiology. More specifically, these gut microbial communities influence neurodevelopment through the microbiota-gut-brain (MGB) axis. We tested the hypothesis that larval amphibian neurodevelopment is affected by the aquatic microbial community present in their housing water. Newly hatched Northern Leopard Frog ( Lithobates pipiens ) tadpoles were raised in pond water that was unmanipulated (natural) or autoclaved. Tadpoles raised in autoclaved pond water had a gut microbiota with reduced bacterial diversity and altered community composition, had decreased behavioural responses to sensory stimuli, were larger in overall body mass, had relatively heavier brains and had altered brain shape when compared with tadpoles raised in natural pond water. Further, the diversity and composition of the gut microbiota were associated with tadpole behavioural responses and brain measurements. Our results suggest that aquatic microbial communities shape tadpole behaviour and brain development, providing strong support for the occurrence of the MGB axis in amphibians. Lastly, the dramatic role played by aquatic microbial communities on vertebrate neurodevelopment and behaviour should be considered in future wildlife conservation efforts.

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Section: Discussionmentioning

confidence: 92%

Something in the water: aquatic microbial communities influence the larval amphibian gut microbiota, neurodevelopment and behaviour

Emerson,

Woodley

2024

Proc. R. Soc. B.

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“…For culturing GFP labeled ATCC 13076, 10 μg/mL erythromycin was added as the selective pressure. As previously descripted 10 , B. bacteriovorus strains were grown on stationary phase prey S. entertidis ATCC 13076 in HEPES buffer (25 mM HEPES free acid, pH7.6) supplemented with 2mM Calcium Chloride at 30 °C with shaking at 200 rpm, after 24 hours, supernatants were filtered through the 0.45 μm syringe filter (Millipore SLHVR33RB) for at least 2 times to harvest pure predator cells.…”

Section: Methodsmentioning

confidence: 99%

“…With the progress of new genetic tools and techniques, luminescent and fluorescent prey are applied to indicate changes in the predator viability and prey population [6][7][8][9] . Other methods such as real-time quantitative PCR 10,11 , flow cytometry 12 and fluorescence visualization 13,14 are utilized to detect the presence and viability of the predator. Even these rapid methods are described, PFU (plaques forming unit) counting on the doublelayer agar plate is still the conventional, economic, and standard methods for detect and quantify the predator viability and biomass 13,15,16 .…”

Section: Introductionmentioning

confidence: 99%

An efficient method for visualizing the plaques ofBdellovibrio bacteriovorus

Zhao,

Xu,

Zhu

2024

Preprint

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Bdellovibrio bacteriovoruspreys upon other Gram-negative bacteria. This obligate predator is recognized as a living antibiotic to control the rising problem of antibiotic resistance. Plaque forming units (PFU) counting is commonly used to determine the viable numbers ofB. bacteriovorus. However, nearly 3-days incubation is always necessary for getting the single, obvious plaques on the double layer agar plate. This time-consuming procedure greatly impedes the purification and enumeration efficiency ofB. bacteriovorus. In this work, we evaluated the advantages of fluorescent prey on the plaque visualization of the predator. Our study reveals that plaques of theB. bacteriovorushave already formed before they could be monitored by eyes on the double layer agar plate. The regular single plaques are easily observed under the enhanced background of fluorescent prey lawn in 1.5 days, reducing nearly half of the time consumption in the purification and enumeration ofB. bacteriovorus. In addition, it also provides some paradigms for modeling and testing the subtle predation process on the solid medium.IMPORTANCEThe viability of predatorBdellovibrio bacteriovorusis typically suggested by the lysis of the prey bacteria on the double layer agar plate. However, long period incubation is necessary to get single obvious plaques ofB. bacteriovorus. Here, we found that plaques are already generated before it can be monitored by eyes. The plaques are obvious on the enhanced background of the double layer agar plate in the presence of fluorescent prey under UV light. We confirmed that the utilization of fluorescence labeled prey bacteria reduces the time spent on the isolation, purification, and enumeration of the predatorB. bacteriovoruson the double layer agar plate.

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Effects of Dietary Melatonin on Antioxidant Capacity, Immune Defense, and Intestinal Microbiota in Red Swamp Crayfish (Procambarus clarkii)

Ye,

Huang,

et al. 2024

Mar Biotechnol

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Bdellovibrio lyse multiple pathogenic bacteria and protect crucian carp Carassius auratus gibelio from Aeromonas veronii infections

Cited by 7 publications

References 35 publications

Something in the water: aquatic microbial communities influence the larval amphibian gut microbiota, neurodevelopment and behaviour

Something in the water: aquatic microbial communities influence the larval amphibian gut microbiota, neurodevelopment and behaviour

An efficient method for visualizing the plaques ofBdellovibrio bacteriovorus

Effects of Dietary Melatonin on Antioxidant Capacity, Immune Defense, and Intestinal Microbiota in Red Swamp Crayfish (Procambarus clarkii)

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